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SH2D4B (NM_207372) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG213455
SH2D4B (tGFP-tagged) - Human SH2 domain containing 4B (SH2D4B), transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $657.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SH2D4B
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG213455 representing NM_207372
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCAGCAGATCCTGCACGACATGTACATCGACCCCGAGCTCCTTGCCGAGCTCAGCGATGTGCAGA
AGCACATCCTCTTCTACAAAATGCGGGAGGAGCAGCTGAGGCGCTGGAAGGAGCGGGAGACTTGGGAGGC
CCTGGCCCAGGACGAGGGTCTCAGGCCTCCAAAGACCAAGCGAGCAGCGAGTGACAAGCACATCCAATGG
CTCCTAGGGGCAGATGGCGAGGTCTGGGTCTGGATCATGGGAGAAGGCCCTGGTGACAAGCCCTACGAAG
AGATCTCTGAGGAGCTGATTGCAGAGAGGGCGCGGCTGCAGGCACAGAGGGAAGCTGAGGAGCTCTGGAG
ACAGAAGGAGGCAGAGATCACCAAGAAGTTCCGGGATGCTCTGGCCAATGAGAAAGCCCGGATCTTGGCG
GAGAAGTGGAAAGTGGAGATGGAAGGCCGCAAGGCTGCCAAAGTCCTGGAGGAACGCATCCACGAGGAAT
TCAAGAGGAAAGAGGAAGAGGAGAGGAAGCGAGGAGAAGAGCGGATTCGCCTCCAGGAAGAGCAGAGGGC
GAAGGAGCTCTACTGGACCCTGAAGCAGGCTCAGCTGCATTGCCAAGCCAGTGAGAAAGAGGAGCGAGAG
TGGGAAGAACAGTTGCGCCGGTCCAAGGCGGCTGATGAGGAGAGGAGCCGCCGAGCCCAGCGCGCCCGGG
ACGAGTACCGACACCACTCGCTCCGTGCTATCCAGAAGGGCACGGTCGCTGGCCTCAGCTCCATGTTCCG
GGAGCTTGGCCAGAGCCATGAGCAGGAGGCAAGACTCTACCACCACCTCCCCGACCCGGGTCTGCCGCAG
CCCCTTGCCCTGCCGGTCAGGACCTGGGAGCGCCCGCTGCGCCCAGTCTCCAGAGATGTCATCGTCCGCT
GGTTTAAGGAGGAGCAGCTGCCTCGCCGAGCTGGCTTCGAGAGGAACACCAAGTTCATCGCCCCCTGGTT
CCATGGAGGAAATTATCACTGTTTCAGGAGGAGAGTTACTTCAGGAACCCTGCGGACAGAGGGACAGCCC
ACCAGACTACCATCTGTTGTT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG213455 representing NM_207372
Red=Cloning site Green=Tags(s)

MLQQILHDMYIDPELLAELSDVQKHILFYKMREEQLRRWKERETWEALAQDEGLRPPKTKRAASDKHIQW
LLGADGEVWVWIMGEGPGDKPYEEISEELIAERARLQAQREAEELWRQKEAEITKKFRDALANEKARILA
EKWKVEMEGRKAAKVLEERIHEEFKRKEEEERKRGEERIRLQEEQRAKELYWTLKQAQLHCQASEKEERE
WEEQLRRSKAADEERSRRAQRARDEYRHHSLRAIQKGTVAGLSSMFRELGQSHEQEARLYHHLPDPGLPQ
PLALPVRTWERPLRPVSRDVIVRWFKEEQLPRRAGFERNTKFIAPWFHGGNYHCFRRRVTSGTLRTEGQP
TRLPSVV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_207372
ORF Size 1071 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_207372.1, NP_997255.1
RefSeq Size 3983 bp
RefSeq ORF 1074 bp
Locus ID 387694
UniProt ID Q5SQS7
Cytogenetics 10q23.1
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Documents
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"NM_207372" in other vectors (4)
SKU Description Size Price
RC213455 SH2D4B (Myc-DDK-tagged)-Human SH2 domain containing 4B (SH2D4B), transcript variant 1 10 ug
$457.00
RC213455L3 Lenti ORF clone of Human SH2 domain containing 4B (SH2D4B), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC213455L4 Lenti ORF clone of Human SH2 domain containing 4B (SH2D4B), transcript variant 1, mGFP tagged 10 ug
$757.00
SC308487 SH2D4B (untagged)-Human SH2 domain containing 4B (SH2D4B), transcript variant 1 10 ug
$503.00

Plasmid Map for RG213455

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG213455
Circular map for RG213455

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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