TRPM1 (NM_002420) Human Tagged ORF Clone

  • TrueORF®
SKU
RG213443
TRPM1 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily M, member 1 (TRPM1)
  $2,256.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TRPM1
Synonyms CSNB1C; LTRPC1; MLSN1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_002420
ORF Size 4809 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002420.6
RefSeq Size 5428 bp
RefSeq ORF 4812 bp
Locus ID 4308
UniProt ID Q7Z4N2
Cytogenetics 15q13.3
Domains ion_trans
Protein Families Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
Summary This gene encodes a member of the transient receptor potential melastatin subfamily of transient receptor potential ion channels. The encoded protein is a calcium permeable cation channel that is expressed in melanocytes and may play a role in melanin synthesis. Specific mutations in this gene are the cause autosomal recessive complete congenital stationary night blindness-1C. The expression of this protein is inversely correlated with melanoma aggressiveness and as such it is used as a prognostic marker for melanoma metastasis. Alternate splicing results in multiple transcript variants. provided by RefSeq, Oct 2011
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"NM_002420" in other vectors (6)
SKU Description Size Price
RC213443 TRPM1 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily M, member 1 (TRPM1) 10 ug
$2,056.00
RC213443L1 Lenti ORF clone of Human transient receptor potential cation channel, subfamily M, member 1 (TRPM1), Myc-DDK-tagged 10 ug
$2,356.00
RC213443L2 Lenti ORF clone of Human transient receptor potential cation channel, subfamily M, member 1 (TRPM1), mGFP tagged 10 ug
$2,356.00
RC213443L3 Lenti ORF clone of Human transient receptor potential cation channel, subfamily M, member 1 (TRPM1), Myc-DDK-tagged 10 ug
$2,356.00
RC213443L4 Lenti ORF clone of Human transient receptor potential cation channel, subfamily M, member 1 (TRPM1), mGFP tagged 10 ug
$2,356.00
SC318643 TRPM1 (untagged)-Human transient receptor potential cation channel, subfamily M, member 1 (TRPM1) 10 ug
$1,372.00

Plasmid Map for RG213443

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Cloning scheme for RG213443

Circular map for RG213443

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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