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DCLK3 (NM_033403) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG213096
DCLK3 (tGFP-tagged) - Human doublecortin-like kinase 3 (DCLK3)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $803.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DCLK3
Synonyms CLR; DCAMKL3; DCDC3C; DCK3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG213096 representing NM_033403
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAAAGAACCACTGACACTGAAGAGCATTCAGGTGGCTGTAGAAGAACTGTACCCCAACAAAGCCC
GGGCCCTGACACTGGCCCAGCACAGCCGTGCCCCTTCTCCAAGGCTGAGGAGCAGGCTGTTTAGCAAGGC
TCTGAAAGGAGACCACCGCTGTGGGGAGACCGAGACCCCCAAGAGCTGCAGCGAAGTTGCAGGATGCAAG
GCAGCCATGAGGCACCAGGGGAAGATCCCCGAGGAGCTTTCACTAGATGACAGAGCGAGGACCCAGAAGA
AGTGGGGGAGGGGGAAATGGGAGCCAGAACCCAGTAGCAAGCCCCCCAGGGAAGCCACTCTGGAAGAGAG
GCACGCAAGGGGAGAGAAGCATCTTGGGGTGGAGATTGAAAAGACCTCGGGTGAAATTATCAGATGCGAG
AAGTGCAAGAGAGAGAGGGAGCTCCAGCAGAGCCTGGAGCGTGAGAGGCTTTCTCTGGGGACCAGTGAGC
TGGATATGGGGAAGGGCCCAATGTATGATGTGGAGAAGCTGGTGAGGACCAGAAGCTGCAGGAGGTCTCC
CGAGGCAAATCCTGCAAGTGGGGAGGAAGGGTGGAAGGGTGACAGCCACAGGAGCAGCCCCAGGAATCCC
ACTCAAGAGCTGAGGAGACCCAGCAAGAGCATGGACAAGAAAGAGGACAGAGGCCCAGAGGATCAAGAAA
GCCATGCTCAGGGAGCAGCCAAGGCCAAGAAGGACCTTGTGGAAGTTCTTCCTGTCACAGAGGAGGGGCT
GAGGGAGGTGAAGAAGGACACCAGGCCCATGAGCAGGAGCAAACATGGTGGCTGGCTCCTGAGAGAGCAC
CAGGCGGGCTTTGAGAAGCTCCGCAGGACCCGAGGAGAAGAGAAGGAGGCAGAGAAGGAGAAAAAGCCAT
GTATGTCTGGAGGCAGAAGGATGACTCTCAGAGATGACCAACCTGCAAAGCTAGAAAAGGAGCCCAAGAC
GAGGCCAGAAGAGAACAAGCCAGAGCGGCCCAGCGGTCGGAAGCCACGGCCCATGGGCATCATTGCCGCC
AATGTGGAAAAGCATTATGAGACTGGCCGGGTCATTGGGGATGGGAACTTTGCTGTCGTGAAGGAGTGCA
GACACCGCGAGACCAGGCAGGCCTATGCGATGAAGATCATTGACAAGTCCAGACTCAAGGGCAAGGAGGA
CATGGTGGACAGTGAGATCTTGATCATCCAGAGCCTCTCTCACCCCAACATCGTGAAATTGCATGAAGTC
TACGAAACAGACATGGAAATCTACCTGATCCTGGAGTACGTGCAGGGAGGAGACCTTTTTGACGCCATCA
TAGAAAGTGTGAAGTTCCCGGAGCCCGATGCTGCCCTCATGATCATGGACTTATGCAAAGCCCTCGTCCA
CATGCACGACAAGAGCATTGTCCACCGGGACCTCAAGCCGGAAAACCTTTTGGTTCAGCGAAATGAGGAC
AAATCTACTACCTTGAAATTGGCTGATTTTGGACTTGCAAAGCATGTGGTGAGACCTATATTTACTGTGT
GTGGGACCCCAACTTACGTAGCTCCCGAAATTCTTTCTGAGAAAGGTTATGGACTGGAGGTGGACATGTG
GGCTGCTGGCGTGATCCTCTATATCCTGCTGTGTGGCTTTCCCCCATTCCGCAGCCCTGAGAGGGACCAG
GACGAGCTCTTTAACATCATCCAGCTGGGCCACTTTGAGTTCCTCCCCCCTTACTGGGACAATATCTCTG
ATGCTGCTAAAGATCTGGTGAGCCGGTTGCTGGTGGTAGACCCCAAAAAGCGCTACACAGCTCATCAGGT
TCTTCAGCACCCCTGGATCGAAACAGCTGGCAAGACCAATACAGTGAAACGACAGAAGCAGGTGTCCCCC
AGCAGCGAGGGTCACTTCCGGAGCCAGCACAAGAGGGTTGTGGAGCAGGTATCA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG213096 representing NM_033403
Red=Cloning site Green=Tags(s)

MGKEPLTLKSIQVAVEELYPNKARALTLAQHSRAPSPRLRSRLFSKALKGDHRCGETETPKSCSEVAGCK
AAMRHQGKIPEELSLDDRARTQKKWGRGKWEPEPSSKPPREATLEERHARGEKHLGVEIEKTSGEIIRCE
KCKRERELQQSLERERLSLGTSELDMGKGPMYDVEKLVRTRSCRRSPEANPASGEEGWKGDSHRSSPRNP
TQELRRPSKSMDKKEDRGPEDQESHAQGAAKAKKDLVEVLPVTEEGLREVKKDTRPMSRSKHGGWLLREH
QAGFEKLRRTRGEEKEAEKEKKPCMSGGRRMTLRDDQPAKLEKEPKTRPEENKPERPSGRKPRPMGIIAA
NVEKHYETGRVIGDGNFAVVKECRHRETRQAYAMKIIDKSRLKGKEDMVDSEILIIQSLSHPNIVKLHEV
YETDMEIYLILEYVQGGDLFDAIIESVKFPEPDAALMIMDLCKALVHMHDKSIVHRDLKPENLLVQRNED
KSTTLKLADFGLAKHVVRPIFTVCGTPTYVAPEILSEKGYGLEVDMWAAGVILYILLCGFPPFRSPERDQ
DELFNIIQLGHFEFLPPYWDNISDAAKDLVSRLLVVDPKKRYTAHQVLQHPWIETAGKTNTVKRQKQVSP
SSEGHFRSQHKRVVEQVS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_033403
ORF Size 1944 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_033403.1, NP_208382.1
RefSeq Size 5344 bp
RefSeq ORF 1947 bp
Locus ID 85443
UniProt ID Q9C098
Cytogenetics 3p22.2
Protein Families Druggable Genome, Protein Kinase
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"NM_033403" in other vectors (7)
SKU Description Size Price
RC213096 DCLK3 (Myc-DDK-tagged)-Human doublecortin-like kinase 3 (DCLK3) 10 ug
$289.00 $603.00
RC213096L1 Lenti ORF clone of Human doublecortin-like kinase 3 (DCLK3), Myc-DDK-tagged 10 ug
$903.00
RC213096L2 Lenti ORF clone of Human doublecortin-like kinase 3 (DCLK3), mGFP tagged 10 ug
$903.00
RC213096L3 Lenti ORF clone of Human doublecortin-like kinase 3 (DCLK3), Myc-DDK-tagged 10 ug
$903.00
RC213096L4 Lenti ORF clone of Human doublecortin-like kinase 3 (DCLK3), mGFP tagged 10 ug
$903.00
SC316560 DCLK3 (untagged)-Human doublecortin-like kinase 3 (DCLK3) 10 ug
$664.00
SC323628 DCLK3 (untagged)-Kinase deficient mutant (K313M) of Human doublecortin-like kinase 3 (DCLK3) 10 ug
$604.00

Plasmid Map for RG213096

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG213096
Circular map for RG213096

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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