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PPEF2 (NM_006239) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG211899
PPEF2 (tGFP-tagged) - Human protein phosphatase, EF-hand calcium binding domain 2 (PPEF2)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $889.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PPEF2
Synonyms PPP7CB
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG211899 representing NM_006239
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAAGCGGCACCTCCACCCAACATCATTTTGCTTTCCAGAATGCAGAGAGAGCCTTCAAGGCAGCAG
CCCTGATCCAGAGATGGTACCGGCGCTACGTGGCCCGCCTGGAGATGAGGCGGCGTTGCACCTGGAGCAT
CTTCCAGTCTATAGAATATGCTGGGCAGCAAGACCAAGTCAAGCTCCATGACTTCTTCAGCTATCTCATG
GATCACTTCATCCCCAGCAGCCACAACGACAGGGACTTCCTGACCCGCATATTCACTGAGGACAGATTCG
CCCAGGACTCCGAGATGAAGAAATGCAGTGACTATGAATCCATAGAGGTACCCGACAGTTACACGGGGCC
ACGCCTCTCCTTCCCACTCCTGCCTGACCATGCAACTGCCCTGGTAGAAGCATTCAGACTGAAACAACAG
CTCCATGCTCGCTACGTCTTGAACCTTTTGTATGAAACCAAGAAACATCTGGTACAGCTGCCAAACATCA
ACCGGGTCTCAACCTGTTACAGTGAGGAGATCACAGTGTGTGGAGACTTACATGGCCAATTGGATGACTT
AATCTTTATATTTTATAAGAATGGCCTCCCGTCGCCAGAACGGTCATATGTGTTCAACGGTGACTTTGTG
GATCGAGGCAAGGATTCAGTAGAGATCCTGATGATTCTTTTTGCCTTCATGCTGGTTTACCCCAAAGAGT
TCCATCTTAACAGAGGAAACCATGAGGACCATATGGTGAACTTACGATATGGCTTCACCAAGGAAGTGAT
GAATAAATACAAGGTACACGGGAAGGAAATACTAAGAACCCTGCAAGATGTTTTCTGTTGGCTTCCACTG
GCCACTCTGATAGATGAGAAAGTTCTAATTCTTCATGGTGGGGTGTCAGACATAACTGATCTGGAGCTTT
TGGACAAAATAGAGAGGAGCAAGATAGTTTCCACCATGAGGTGCAAAACGAGACAGAAGAGTGAGAAGCA
GATGGAGGAGAAGAGAAGAGCCAACCAGAAGAGCTCTGCACAGGGACCCATCCCATGGTTTCTCCCCGAA
AGCCGCTCTCTTCCCTCTTCGCCCCTTCGGCTTGGCTCCTACAAGGCCCAGAAAACCAGCAGGTCCTCCA
GCATCCCCTGCAGCGGTTCCCTGGACGGGCGGGAGCTCTCCCGGCAGGTGCGGAGCTCCGTGGAACTGGA
GCTAGAGCGGTGCCGGCAGCAAGCAGGCCTCCTGGTGACCGGAGAGAAAGAGGAGCCCTCCCGCTCAGCC
TCAGAAGCAGACTCTGAAGCCGGAGAGCTGCGGAAGCCCACTCAGGAGGAGTGGAGGCAGGTTGTAGATA
TCCTGTGGAGTGATCCCATGGCTCAAGAGGGCTGCAAGGCCAACACTATTCGAGGAGGAGGCTGTTATTT
TGGGCCTGATGTGACACAACAGTTGCTACAAAAATACAACATGCAATTCCTGATCCGTTCACATGAATGC
AAACCTGAAGGCTATGAATTCTGTCACAACCGCAAGGTATTAACAATCTTTTCTGCCTCCAACTACTATG
AAGTTGGCAGCAACAGAGGGGCCTATGTCAAACTGGGGCCAGCCCTGACCCCACATATTGTGCAGTATCA
AGCTAACAAGGTGACCCACACACTCACCATGAGGCAAAGGATTAGCAGAGTGGAGGAGTCGGCTCTGAGA
GCTCTGAGGGAGAAGCTGTTTGCTCATTCTTCAGATCTTCTCAGTGAATTTAAGAAGCATGATGCAGATA
AAGTCGGTTTAATCACCTTGAGTGACTGGGCAGCAGCGGTGGAGTCTGTGTTGCACCTAGGACTGCCATG
GCGGATGCTGAGGCCACAGCTGGTGAACAGCTCAGCAGACAACATGCTGGAGTACAAGTCTTGGCTGAAG
AACTTGGCCAAGGAACAACTGAGTCGCGAGAACATACAATCAAGTTTGCTGGAAACATTGTATCGAAACC
GATCCAACCTAGAGACCATTTTTAGGATCATAGACAGTGATCATTCAGGGTTCATCTCACTGGACGAGTT
CAGGCAGACCTGGAAGCTGTTCAGCTCTCACATGAATATCGACATTACAGATGACTGCATCTGTGACCTT
GCTCGGAGCATTGATTTCAACAAAGATGGCCACATTGATATCAATGAGTTCCTGGAGGCCTTCCGCCTTG
TGGAGAAATCCTGCCCAGAGGGCGATGCCTCAGAATGCCCACAAGCTACAAATGCTAAAGACAGTGGCTG
CAGCAGTCCAGGTGCACAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG211899 representing NM_006239
Red=Cloning site Green=Tags(s)

MGSGTSTQHHFAFQNAERAFKAAALIQRWYRRYVARLEMRRRCTWSIFQSIEYAGQQDQVKLHDFFSYLM
DHFIPSSHNDRDFLTRIFTEDRFAQDSEMKKCSDYESIEVPDSYTGPRLSFPLLPDHATALVEAFRLKQQ
LHARYVLNLLYETKKHLVQLPNINRVSTCYSEEITVCGDLHGQLDDLIFIFYKNGLPSPERSYVFNGDFV
DRGKDSVEILMILFAFMLVYPKEFHLNRGNHEDHMVNLRYGFTKEVMNKYKVHGKEILRTLQDVFCWLPL
ATLIDEKVLILHGGVSDITDLELLDKIERSKIVSTMRCKTRQKSEKQMEEKRRANQKSSAQGPIPWFLPE
SRSLPSSPLRLGSYKAQKTSRSSSIPCSGSLDGRELSRQVRSSVELELERCRQQAGLLVTGEKEEPSRSA
SEADSEAGELRKPTQEEWRQVVDILWSDPMAQEGCKANTIRGGGCYFGPDVTQQLLQKYNMQFLIRSHEC
KPEGYEFCHNRKVLTIFSASNYYEVGSNRGAYVKLGPALTPHIVQYQANKVTHTLTMRQRISRVEESALR
ALREKLFAHSSDLLSEFKKHDADKVGLITLSDWAAAVESVLHLGLPWRMLRPQLVNSSADNMLEYKSWLK
NLAKEQLSRENIQSSLLETLYRNRSNLETIFRIIDSDHSGFISLDEFRQTWKLFSSHMNIDITDDCICDL
ARSIDFNKDGHIDINEFLEAFRLVEKSCPEGDASECPQATNAKDSGCSSPGAH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006239
ORF Size 2259 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006239.3
RefSeq Size 3430 bp
RefSeq ORF 2262 bp
Locus ID 5470
UniProt ID O14830
Cytogenetics 4q21.1
Domains EFh, IQ, Metallophos, PP2Ac
Protein Families Druggable Genome
Summary This gene encodes a member of the serine/threonine protein phosphatase with EF-hand motif family. The protein contains a protein phosphatase catalytic domain, and at least two EF-hand calcium-binding motifs in its C terminus. Although its substrate(s) is unknown, the encoded protein, which is expressed specifically in photoreceptors and the pineal, has been suggested to play a role in the visual system. This gene shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. provided by RefSeq, Jul 2008
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"NM_006239" in other vectors (6)
SKU Description Size Price
RC211899 PPEF2 (Myc-DDK-tagged)-Human protein phosphatase, EF-hand calcium binding domain 2 (PPEF2) 10 ug
$689.00
RC211899L1 Lenti ORF clone of Human protein phosphatase, EF-hand calcium binding domain 2 (PPEF2), Myc-DDK-tagged 10 ug
$989.00
RC211899L2 Lenti ORF clone of Human protein phosphatase, EF-hand calcium binding domain 2 (PPEF2), mGFP tagged 10 ug
$989.00
RC211899L3 Lenti ORF clone of Human protein phosphatase, EF-hand calcium binding domain 2 (PPEF2), Myc-DDK-tagged 10 ug
$989.00
RC211899L4 Lenti ORF clone of Human protein phosphatase, EF-hand calcium binding domain 2 (PPEF2), mGFP tagged 10 ug
$989.00
SC314610 PPEF2 (untagged)-Human protein phosphatase, EF-hand calcium binding domain 2 (PPEF2) 10 ug
$759.00

Plasmid Map for RG211899

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG211899
Circular map for RG211899

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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