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SENP6 (NM_015571) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG211809
SENP6 (tGFP-tagged) - Human SUMO1/sentrin specific peptidase 6 (SENP6), transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,168.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SENP6
Synonyms SSP1; SUSP1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG211809 representing NM_015571
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATT
CGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC

ATGGCGGCCGGCAAGAGCGGCGGTAGCGCAGGGGAGATTACTTTTCTGGAAGCTTTGGCT
AGATCAGAGTCTAAGAGAGATGGAGGTTTTAAAAATAATTGGAGCTTTGATCATGAAGAA
GAAAGTGAAGGAGATACAGATAAAGATGGGACAAATCTGCTCAGTGTGGATGAAGATGAG
GATTCTGAAACCTCAAAAGGAAAAAAGTTAAATCGTCGATCTGAAATTGTTGCTAATAGC
TCTGGTGAATTCATCTTGAAGACATATGTAAGACGAAACAAGTCTGAAAGTTTTAAAACT
TTGAAAGGCAACCCAATTGGACTTAACATGTTGAGCAACAATAAGAAATTGAGTGAAAAT
ACGCAAAATACGTCATTATGTTCTGGAACTGTAGTTCATGGTAGACGTTTTCATCATGCT
CATGCACAGATACCAGTAGTAAAAACAGCAGCCCAAAGCAGTCTGGACCGAAAAGAAAGG
AAAGAATACCCACCTCATGTCCAAAAAGTTGAAATTAATCCTGTAAGGTTAAGTCGGCTC
CAAGGTGTTGAACGTATAATGAAGAAAACAGAAGAGTCCGAATCACAAGTGGAGCCTGAA
ATTAAGAGGAAAGTACAACAGAAACGACACTGTAGTACCTATCAGCCTACTCCTCCTCTA
TCTCCTGCTTCAAAAAAATGTTTAACCCATTTAGAGGATTTGCAAAGAAATTGCAGACAA
GCTATTACTTTGAATGAGTCTACTGGACCATTATTAAGAACGTCAATTCATCAGAATTCT
GGAGGACAGAAGTCACAAAACACAGGATTAACAACCAAGAAGTTTTATGGCAACAATGTG
GAAAAGGTTCCAATTGATATTATTGTGAATTGTGATGACAGTAAACACACTTATTTACAG
ACTAATGGAAAAGTCATTTTACCTGGGGCAAAAATACCCAAAATCACAAACTTGAAAGAA
AGGAAAACAAGTTTGTCAGACCTAAATGATCCAATCATTTTGTCCAGTGATGATGATGAT
GACAACGACAGAACTAACAGAAGAGAAAGCATATCTCCTCAGCCTGCTGATTCAGCATGT
TCTTCCCCTGCACCATCCACTGGAAAAGTAGAAGCAGCGCTAAATGAAAATACTTGCAGA
GCAGAGCGTGAACTACGAAGCATTCCAGAAGACTCAGAGTTAAATACAGTTACATTGCCA
AGAAAAGCAAGAATGAAAGACCAGTTTGGCAATTCTATTATCAACACACCTCTGAAACGT
CGTAAAGTGTTTTCTCAAGAACCTCCAGATGCTTTAGCTTTAAGCTGCCAAAGTTCCTTT
GACAGTGTCATTTTAAACTGTCGAAGTATACGAGTAGGAACACTCTTCCGGCTGTTAATA
GAGCCTGTAATTTTTTGTTTAGATTTTATCAAGATACAGCTAGACGAACCAGACCATGAT
CCTGTAGAGATTATATTAAATACCTCTGATCTAACTAAATGTGAATGGTGTAATGTCCGA
AAATTACCTGTAGTGTTTCTTCAAGCAATTCCAGCAGTTTATCAAAAGCTGAGCATCCAA
CTGCAAATGAATAAGGAGGATAAAGTTTGGAATGATTGTAAAGGAGTAAATAAATTAACA
AATTTAGAAGAACAATATATAATTTTAATTTTTCAAAATGGCCTTGATCCTCCGGCAAAT
ATGGTATTTGAAAGTATCATTAATGAAATTGGTATAAAGAATAACATCTCCAATTTTTTT
GCGAAAATTCCCTTTGAAGAAGCTAATGGCAGACTTGTTGCCTGTACAAGAACCTATGAA
GAGAGCATCAAAGGAAGTTGTGGGCAAAAGGAAAACAAAATTAAAACTGTATCATTTGAA
TCTAAAATACAACTTAGAAGCAAACAAGAATTTCAGTTTTTTGATGAAGAAGAAGAAACT
GGAGAAAACCACACCATCTTCATTGGCCCAGTAGAAAAGTTGATAGTATATCCACCACCT
CCAGCTAAGGGAGGCATCTCTGTTACCAATGAGGACCTGCACTGTCTAAATGAAGGAGAA
TTTTTAAATGATGTTATTATAGACTTTTATTTGAAATACTTGGTGCTTGAAAAACTGAAG
AAGGAAGACGCTGACCGAATTCATATATTCAGTTCTTTTTTCTATAAACGCCTTAATCAG
AGAGAGAGGAGAAATCATGAAACAACTAATCTGTCAATACAGCAAAAACGGCATGGGAGA
GTAAAAACATGGACCCGGCACGTAGATATTTTTGAGAAGGATTTTATTTTTGTACCCCTT
AATGAAGCTGCACACTGGTTTTTGGCTGTTGTTTGTTTCCCCGGTTTGGAAAAACCAAAG
TATGAACCTAATCCTCATTACCATGAAAATGCTGTCATACAGAAATGTTCAACTGTAGAG
GACAGTTGTATTTCTTCTTCAGCCAGTGAAATGGAGAGTTGTTCACAAAACTCTTCTGCC
AAGCCTGTAATTAAGAAGATGCTAAACAAAAAACATTGCATAGCTGTAATTGATTCCAAT
CCTGGGCAGGAAGAAAGTGACCCTCGTTATAAGAGAAACATATGCAGTGTAAAATACAGT
GTGAAAAAAATAAATCATACTGCGAGTGAAAATGAAGAATTCAATAAAGGAGAATCTACA
TCCCAGAAAGTTGCTGATAGGACTAAAAGTGAGAATGGCCTACAGAATGAAAGTTTAAGT
TCCACACATCATACAGATGGCTTAAGCAAAATCAGACTAAACTATAGCGATGAATCACCT
GAAGCTGGTAAAATGCTTGAAGATGAACTCGTCGACTTCTCAGAAGATCAGGATAACCAG
GATGATAGCAGTGACGATGGATTCCTCGCTGATGACAACTGCAGTTCAGAAATAGGACAG
TGGCATTTAAAGCCTACTATCTGTAAACAACCTTGTATCCTACTTATGGACTCACTCCGA
GGCCCTTCTCGGTCAAATGTTGTCAAAATTTTAAGAGAGTATTTAGAAGTGGAATGGGAA
GTTAAAAAAGGAAGCAAAAGAAGTTTTTCCAAAGATGTTATGAAGGGCTCTAATCCAAAA
GTACCACAGCAAAACAACTTCAGTGACTGTGGTGTATATGTATTGCAGTATGTAGAGAGC
TTTTTTGAGAATCCAATTCTCAGTTTTGAACTACCTATGAATTTGGCAAACTGGTTTCCT
CCACCAAGAATGAGAACAAAAAGAGAAGAAATCCGAAACATAATTCTGAAGCTACAGGAA
GATCAGAGCAAAGAGAAAAGAAAGCATAAGGACACTTACTCAACAGAAGCACCTTTAGGC
GAAGGAACAGAACAATATGTCAATAGTATCTCAGAT

ACGCGTACGCGGCCGCTCGAGATGGAGAGCGACGAGAGCGGCCTGCCCGCCATGGAGATCGAGTGCCGCATCACCGGCACC
CTGAACGGCGTGGAGTTCGAGCTG
Protein Sequence
>RG211809 protein sequence
Blue=ORF Red=Cloning site Green=Tag(s)

MAAGKSGGSAGEITFLEALARSESKRDGGFKNNWSFDHEEESEGDTDKDGTNLLSVDEDE
DSETSKGKKLNRRSEIVANSSGEFILKTYVRRNKSESFKTLKGNPIGLNMLSNNKKLSEN
TQNTSLCSGTVVHGRRFHHAHAQIPVVKTAAQSSLDRKERKEYPPHVQKVEINPVRLSRL
QGVERIMKKTEESESQVEPEIKRKVQQKRHCSTYQPTPPLSPASKKCLTHLEDLQRNCRQ
AITLNESTGPLLRTSIHQNSGGQKSQNTGLTTKKFYGNNVEKVPIDIIVNCDDSKHTYLQ
TNGKVILPGAKIPKITNLKERKTSLSDLNDPIILSSDDDDDNDRTNRRESISPQPADSAC
SSPAPSTGKVEAALNENTCRAERELRSIPEDSELNTVTLPRKARMKDQFGNSIINTPLKR
RKVFSQEPPDALALSCQSSFDSVILNCRSIRVGTLFRLLIEPVIFCLDFIKIQLDEPDHD
PVEIILNTSDLTKCEWCNVRKLPVVFLQAIPAVYQKLSIQLQMNKEDKVWNDCKGVNKLT
NLEEQYIILIFQNGLDPPANMVFESIINEIGIKNNISNFFAKIPFEEANGRLVACTRTYE
ESIKGSCGQKENKIKTVSFESKIQLRSKQEFQFFDEEEETGENHTIFIGPVEKLIVYPPP
PAKGGISVTNEDLHCLNEGEFLNDVIIDFYLKYLVLEKLKKEDADRIHIFSSFFYKRLNQ
RERRNHETTNLSIQQKRHGRVKTWTRHVDIFEKDFIFVPLNEAAHWFLAVVCFPGLEKPK
YEPNPHYHENAVIQKCSTVEDSCISSSASEMESCSQNSSAKPVIKKMLNKKHCIAVIDSN
PGQEESDPRYKRNICSVKYSVKKINHTASENEEFNKGESTSQKVADRTKSENGLQNESLS
STHHTDGLSKIRLNYSDESPEAGKMLEDELVDFSEDQDNQDDSSDDGFLADDNCSSEIGQ
WHLKPTICKQPCILLMDSLRGPSRSNVVKILREYLEVEWEVKKGSKRSFSKDVMKGSNPK
VPQQNNFSDCGVYVLQYVESFFENPILSFELPMNLANWFPPPRMRTKREEIRNIILKLQE
DQSKEKRKHKDTYSTEAPLGEGTEQYVNSISD

TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLS
HVMGYGFYHFGTYPSGYEN
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015571
ORF Size 3336 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015571.4
RefSeq Size 4210 bp
RefSeq ORF 3339 bp
Locus ID 26054
UniProt ID Q9GZR1
Cytogenetics 6q14.1
Domains Peptidase_C48
Protein Families Druggable Genome, Protease
Summary Ubiquitin-like molecules (UBLs), such as SUMO1 (UBL1; MIM 601912), are structurally related to ubiquitin (MIM 191339) and can be ligated to target proteins in a similar manner as ubiquitin. However, covalent attachment of UBLs does not result in degradation of the modified proteins. SUMO1 modification is implicated in the targeting of RANGAP1 (MIM 602362) to the nuclear pore complex, as well as in stabilization of I-kappa-B-alpha (NFKBIA; MIM 164008) from degradation by the 26S proteasome. Like ubiquitin, UBLs are synthesized as precursor proteins, with 1 or more amino acids following the C-terminal glycine-glycine residues of the mature UBL protein. Thus, the tail sequences of the UBL precursors need to be removed by UBL-specific proteases, such as SENP6, prior to their conjugation to target proteins (Kim et al., 2000 PubMed 10799485). SENPs also display isopeptidase activity for deconjugation of SUMO-conjugated substrates (Lima and Reverter, 2008 PubMed 18799455).supplied by OMIM, Jun 2009
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"NM_015571" in other vectors (6)
SKU Description Size Price
RC211809 SENP6 (Myc-DDK-tagged)-Human SUMO1/sentrin specific peptidase 6 (SENP6), transcript variant 1 10 ug
$968.00
RC211809L1 Lenti ORF clone of Human SUMO1/sentrin specific peptidase 6 (SENP6), transcript variant 1, Myc-DDK-tagged 10 ug
$1,268.00
RC211809L2 Lenti ORF clone of Human SUMO1/sentrin specific peptidase 6 (SENP6), transcript variant 1, mGFP tagged 10 ug
$1,268.00
RC211809L3 Lenti ORF clone of Human SUMO1/sentrin specific peptidase 6 (SENP6), transcript variant 1, Myc-DDK-tagged 10 ug
$1,268.00
RC211809L4 Lenti ORF clone of Human SUMO1/sentrin specific peptidase 6 (SENP6), transcript variant 1, mGFP tagged 10 ug
$1,268.00
SC315191 SENP6 (untagged)-Human SUMO1/sentrin specific peptidase 6 (SENP6), transcript variant 1 10 ug
$1,066.00

Plasmid Map for RG211809

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG211809
Circular map for RG211809

file-alt Citations

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