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C21orf34 (NM_001005734) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG211696
C21orf34 (tGFP-tagged) - Human chromosome 21 open reading frame 34 (C21orf34), transcript variant 3
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $350.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol C21orf34
Synonyms C21orf35; FLJ38295
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG211696 representing NM_001005734
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAATTATATTAGAACTTCAGCAAAGTGCACAGCTGCATAATAGAGAAGCCGGCACTCAGATTGACA
GGCAAATAAGCCAGCTTGTGGAAGGGGCCTCTGGGTGGCAGCTCTGGGCTTTTGGGTGTTGGACAACCAT
AGGCAAAACTGAAATGGAACAGGGGCAGAAAATAGCACACTTTTACTCTACGCAACTGTTGCTTTTTCAT
GATCTTCAGGAATTTTACTGGGATAATTATCCAAATAAATTGCAAGCATTCTATCCAAATGGAGCTCTTT
CTGAGATGAAGAGAATTCTCAATGTCAAGATT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG211696 representing NM_001005734
Red=Cloning site Green=Tags(s)

MEIILELQQSAQLHNREAGTQIDRQISQLVEGASGWQLWAFGCWTTIGKTEMEQGQKIAHFYSTQLLLFH
DLQEFYWDNYPNKLQAFYPNGALSEMKRILNVKI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001005734
ORF Size 312 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001005734.1, NP_001005734.1
RefSeq Size 726 bp
RefSeq ORF 314 bp
Locus ID 388815
Cytogenetics 21q21.1
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"NM_001005734" in other vectors (3)
SKU Description Size Price
RC211696 C21orf34 (Myc-DDK-tagged)-Human chromosome 21 open reading frame 34 (C21orf34), transcript variant 3 10 ug
$150.00
RC211696L3 Lenti ORF clone of Human chromosome 21 open reading frame 34 (C21orf34), transcript variant 3, Myc-DDK-tagged 10 ug
$450.00
RC211696L4 Lenti ORF clone of Human chromosome 21 open reading frame 34 (C21orf34), transcript variant 3, mGFP tagged 10 ug
$450.00

Plasmid Map for RG211696

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Cloning scheme for RG211696
Circular map for RG211696

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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