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MTCP1 (NM_014221) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG211540
MTCP1 (tGFP-tagged) - Human mature T-cell proliferation 1 (MTCP1), nuclear gene encoding mitochondrial protein, transcript variant B1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $350.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MTCP1
Synonyms C6.1B; mature T-cell proliferation 1; OTTHUMP00000015489; OTTHUMP00000015491; OTTHUMP00000015492; OTTHUMP00000024241; OTTHUMP00000082776; P13MTCP1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG211540 representing NM_014221
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCAGAAGGATCCGTGCCAGAAGCAAGCCTGTGAGATACAGAAATGTTTACAAGCCAACAGCTACA
TGGAATCAAAGTGTCAGGCTGTCATCCAAGAACTGCGTAAGTGTTGTGCTCAGTATCCCAAGGGAAGATC
TGTCGTCTGTTCAGGATTTGAAAAAGAAGAGGAAGAAAACCTAACACGGAAGTCTGCATCAAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG211540 representing NM_014221
Red=Cloning site Green=Tags(s)

MPQKDPCQKQACEIQKCLQANSYMESKCQAVIQELRKCCAQYPKGRSVVCSGFEKEEEENLTRKSASK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014221
ORF Size 204 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014221.3, NP_055036.1
RefSeq Size 1943 bp
RefSeq ORF 206 bp
Locus ID 4515
Cytogenetics Xq28
Domains TCL1_MTCP1
Summary This gene was identified by involvement in some t(X;14) translocations associated with mature T-cell proliferations. This region has a complex gene structure, with a common promoter and 5' exon spliced to two different sets of 3' exons that encode two different proteins. This gene represents the upstream 13 kDa protein that is a member of the TCL1 family. This protein may be involved in leukemogenesis. provided by RefSeq, Mar 2009
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"NM_014221" in other vectors (3)
SKU Description Size Price
RC211540 MTCP1 (Myc-DDK-tagged)-Human mature T-cell proliferation 1 (MTCP1), nuclear gene encoding mitochondrial protein, transcript variant B1 10 ug
$150.00
RC211540L3 Lenti ORF clone of Human mature T-cell proliferation 1 (MTCP1), nuclear gene encoding mitochondrial protein, transcript variant B1, Myc-DDK-tagged 10 ug
$450.00
RC211540L4 Lenti ORF clone of Human mature T-cell proliferation 1 (MTCP1), nuclear gene encoding mitochondrial protein, transcript variant B1, mGFP tagged 10 ug
$450.00

Plasmid Map for RG211540

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for RG211540
Circular map for RG211540

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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