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FBXO9 (NM_012347) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG211381
FBXO9 (tGFP-tagged) - Human F-box protein 9 (FBXO9), transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $657.00
3 Weeks*
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Other products for "FBXO9 or NM_012347"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FBXO9
Synonyms dJ341E18.2; FBX9; NY-REN-57; VCIA1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG211381 representing NM_012347
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTGACATTATTTGGGTTTTCCCCCCTCAGGCAGAAGCTGAGGAAGATTGTCATTCTGATACTGTCA
GAGCAGATGATGATGAAGAAAATGAAAGTCCTGCTGAAACAGATCTGCAGGCACAACTCCAGATGTTCCG
AGCTCAGTGGATGTTTGAACTTGCTCCAGGTGTAAGCTCTAGCAATTTAGAAAATCGACCTTGCAGAGCA
GCAAGAGGCTCTCTCCAGAAAACATCGGCAGATACCAAAGGAAAACAAGAACAGGCAAAAGAAGAAAAGG
CTCGAGAACTCTTCCTAAAAGCAGTAGAAGAAGAACAAAATGGAGCTCTCTATGAAGCCATCAAGTTTTA
TCGTAGGGCTATGCAACTTGTACCTGATATAGAGTTCAAGATTACTTATACCCGGTCTCCAGATGGTGAT
GGCGTTGGAAACAGCTACATTGAAGATAATGATGATGACAGCAAAATGGCAGATCTCTTGTCCTACTTCC
AGCAGCAACTCACATTTCAGGAGTCTGTGCTTAAACTGTGTCAGCCTGAGCTTGAGAGCAGTCAGATTCA
CATATCAGTGCTGCCAATGGAGGTCCTGATGTACATCTTCCGATGGGTGGTGTCTAGTGACTTGGACCTC
AGATCATTGGAGCAGTTGTCGCTGGTGTGCAGAGGATTCTACATCTGTGCCAGAGACCCTGAAATATGGC
GTCTGGCCTGCTTGAAAGTTTGGGGCAGAAGCTGTATTAAACTTGTTCCGTACACGTCCTGGAGAGAGAT
GTTTTTAGAACGGCCTCGTGTTCGGTTTGATGGCGTGTATATCAGTAAAACCACATATATTCGTCAAGGG
GAACAGTCTCTTGATGGTTTCTATAGAGCCTGGCACCAAGTGGAATATTACAGGTACATAAGATTCTTTC
CTGATGGCCATGTGATGATGTTGACAACCCCTGAAGAGCCTCAGTCCATTGTTCCACGTTTAAGAACTAG
GAATACCAGGACTGATGCAATTCTACTGGGTCACTATCGCTTGTCACAAGACACAGACAATCAGACCAAA
GTATTTGCTGTAATAACTAAGAAAAAAGAAGAAAAACCACTTGACTATAAATACAGATATTTTCGTCGTG
TCCCTGTACAAGAAGCAGATCAGAGTTTTCATGTGGGGCTACAGCTATGTTCCAGTGGTCACCAGAGGTT
CAACAAACTCATCTGGATACATCATTCTTGTCACATTACTTACAAATCAACTGGTGAGACTGCAGTCAGT
GCTTTTGAGATTGACAAGATGTACACCCCCTTGTTCTTCGCCAGAGTAAGGAGCTACACAGCTTTCTCAG
AAAGGCCTCTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG211381 representing NM_012347
Red=Cloning site Green=Tags(s)

MPDIIWVFPPQAEAEEDCHSDTVRADDDEENESPAETDLQAQLQMFRAQWMFELAPGVSSSNLENRPCRA
ARGSLQKTSADTKGKQEQAKEEKARELFLKAVEEEQNGALYEAIKFYRRAMQLVPDIEFKITYTRSPDGD
GVGNSYIEDNDDDSKMADLLSYFQQQLTFQESVLKLCQPELESSQIHISVLPMEVLMYIFRWVVSSDLDL
RSLEQLSLVCRGFYICARDPEIWRLACLKVWGRSCIKLVPYTSWREMFLERPRVRFDGVYISKTTYIRQG
EQSLDGFYRAWHQVEYYRYIRFFPDGHVMMLTTPEEPQSIVPRLRTRNTRTDAILLGHYRLSQDTDNQTK
VFAVITKKKEEKPLDYKYRYFRRVPVQEADQSFHVGLQLCSSGHQRFNKLIWIHHSCHITYKSTGETAVS
AFEIDKMYTPLFFARVRSYTAFSERPL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012347
ORF Size 1341 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012347.4
RefSeq Size 3164 bp
RefSeq ORF 1344 bp
Locus ID 26268
UniProt ID Q9UK97
Cytogenetics 6p12.1
Domains F-box
Protein Families Druggable Genome
Summary This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates at least 3 transcript variants diverging at the 5' terminus. provided by RefSeq, Jul 2008
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"NM_012347" in other vectors (6)
SKU Description Size Price
RC211381 FBXO9 (Myc-DDK-tagged)-Human F-box protein 9 (FBXO9), transcript variant 1 10 ug
$457.00
RC211381L1 Lenti ORF clone of Human F-box protein 9 (FBXO9), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC211381L2 Lenti ORF clone of Human F-box protein 9 (FBXO9), transcript variant 1, mGFP tagged 10 ug
$757.00
RC211381L3 Lenti ORF clone of Human F-box protein 9 (FBXO9), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC211381L4 Lenti ORF clone of Human F-box protein 9 (FBXO9), transcript variant 1, mGFP tagged 10 ug
$757.00
SC310378 FBXO9 (untagged)-Human F-box protein 9 (FBXO9), transcript variant 1 10 ug
$503.00

Plasmid Map for RG211381

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG211381
Circular map for RG211381

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