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EFCAB3 (NM_173503) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG211009
EFCAB3 (tGFP-tagged) - Human EF-hand calcium binding domain 3 (EFCAB3), transcript variant 2
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $657.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EFCAB3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG211009 representing NM_173503
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGTTTCAGAAATTAAACCAAAACTTAAGCTGAATCCTCTAACAAAAGTACCCATCTCCCACAATA
AAAGGGATAGAGACTTACCAGGATCTCTTCAATGCCAATTACAACACAAAGAAAAGAAGCTAAGTGCTTC
ACAAATGGCAGCTTTCCAAGATGCCTACAACTTCTTCTACAAGGACAAAACTGGCTGTATTGATTTCCAT
GGACTGATGTGCACTGTAGCTAAGCTGGGAATGAATCTGACCAAGCATGATGTCTATAATGAATTGAAAT
GTGCTGATATTGATCGAGATGGGAAGGTGAACTTCTCAGACTTTATCAAGGTTCTAACAGATAAGAATCT
CTTCCTCAAGGCAGTGGTTCCAGAAAAGGAGACCTGTTTAGATTTGGCTGGCAACCCAGGAATCCTATTG
TTTGAAATCCTATCAAGGCTTCTAGAGACTTCAGCCCTACCCAGAAAGTCTATAATAGAAATAGTAAGCT
ATTTCCAAAGAAAATTCCAGCATACTGGCCCAGGAATGTTGTGGAGTCCCTACACTATGGGCTATGGAAA
AAGGACACTTAAGCCAGACATATGCACACCTCCAAGCTCAAGCATGGCTGCCTTTGCTAATGCTGCCCGG
ATTGCAATAATGAAAGAAAAGGATTTATTTAAATTTCTTGAAGAGCTCAAGAGATGCAATTCCGGTTCAG
ATAGCCCATATTCAAAAATACCCATCTTTCCATTGTTCCCTAATGTGGATGGGGTGGTGATGGGAAAGCC
ATTCAAAGACATGCAGAAATTAGAGATGCTAAGAATAAAGGAGCCTTTGCATTTCTTTGAGGATTATTTT
TTCCATAAAAGAGACTGGAAAACACAGGCAGCAAATATAAAGTCTATGGACCCTGCCTCAGGTTATTCAA
ATAACATCTTCACCATTGATCAAATGCTCAAGAAAAAGCAGACTTGTACAGTGGCCGATGCAACTGCTAT
TAAACAACATGTGAAGAGAGCTACTGATACCTATAATTTAGGAATTGCCCTTGAACACCGAAAAGAGATG
CTAAACCTCTGGCAGAAGATCCGAGGGGATTTGATTGGGATGGACTCTAGAAATGAGTCCTTTTATGACA
CCTTTTCTACTTATACATGGTCCTGGAATGTTTGCCAAGAATTACTTTCTCCTAAGGACTTAAGGTTATA
TGATGCCTATGTGAATAGAAATTCCTCCCATAACTCCAGATCCTCTTCCTCATCAGATACCAGTGAATGT
TACACAGACTCAGGAAGAAAAAGAAAACGGAAAGGTTTAAAGGGATTTCAACAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG211009 representing NM_173503
Red=Cloning site Green=Tags(s)

MAVSEIKPKLKLNPLTKVPISHNKRDRDLPGSLQCQLQHKEKKLSASQMAAFQDAYNFFYKDKTGCIDFH
GLMCTVAKLGMNLTKHDVYNELKCADIDRDGKVNFSDFIKVLTDKNLFLKAVVPEKETCLDLAGNPGILL
FEILSRLLETSALPRKSIIEIVSYFQRKFQHTGPGMLWSPYTMGYGKRTLKPDICTPPSSSMAAFANAAR
IAIMKEKDLFKFLEELKRCNSGSDSPYSKIPIFPLFPNVDGVVMGKPFKDMQKLEMLRIKEPLHFFEDYF
FHKRDWKTQAANIKSMDPASGYSNNIFTIDQMLKKKQTCTVADATAIKQHVKRATDTYNLGIALEHRKEM
LNLWQKIRGDLIGMDSRNESFYDTFSTYTWSWNVCQELLSPKDLRLYDAYVNRNSSHNSRSSSSSDTSEC
YTDSGRKRKRKGLKGFQQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173503
ORF Size 1314 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173503.4
RefSeq Size 1543 bp
RefSeq ORF 1317 bp
Locus ID 146779
UniProt ID Q8N7B9
Cytogenetics 17q23.2
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Documents
Resources
"NM_173503" in other vectors (4)
SKU Description Size Price
RC211009 EFCAB3 (Myc-DDK-tagged)-Human EF-hand calcium binding domain 3 (EFCAB3), transcript variant 2 10 ug
$457.00
RC211009L3 Lenti ORF clone of Human EF-hand calcium binding domain 3 (EFCAB3), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC211009L4 Lenti ORF clone of Human EF-hand calcium binding domain 3 (EFCAB3), transcript variant 2, mGFP tagged 10 ug
$757.00
SC306838 EFCAB3 (untagged)-Human EF-hand calcium binding domain 3 (EFCAB3), transcript variant 2 10 ug
$503.00

Plasmid Map for RG211009

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG211009
Circular map for RG211009

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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