OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

VPS26B (NM_052875) Human Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
RG210499
VPS26B (tGFP-tagged) - Human vacuolar protein sorting 26 homolog B (S. pombe) (VPS26B)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA316005 Rabbit polyclonal anti-VP26B antibody
100 ul
$380.00
TA150041 Mouse monoclonal turboGFP antibody, clone OTI2H8 100 ul
$447.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100010 pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
10 ug
$780.00

Other products for "VPS26B or NM_052875"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol VPS26B
Synonyms Pep8b
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG210499 representing NM_052875
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTTCTTCGGCTTCGGGCAGAGCGTGGAGGTGGAAATCCTTCTGAACGATGCAGAGAGTAGGAAGC
GGGCCGAGCACAAGACGGAGGACGGGAAGAAGGAGAAATATTTCCTCTTCTACGACGGGGAGACGGTCTC
CGGGAAGGTGAGCCTTGCCCTCAGGAACCCCAACAAGCGGCTGGAGCACCAGGGCATCAAGATCGAGTTC
ATCGGGCAGATCGAACTCTACTACGATCGCGGGAACCACCATGAGTTTGTGTCCCTGGTGAAGGACCTGG
CCCGGCCTGGAGAGATCACCCAGTCGCAGGCCTTCGACTTTGAGTTTACCCACGTGGAGAAGCCGTATGA
GTCCTACACAGGGCAGAATGTGAAGCTACGCTATTTCCTTCGTGCTACCATCAGCCGCCGCCTCAATGAT
GTTGTCAAAGAGATGGACATTGTAGTTCACACACTCAGCACATACCCAGAGCTGAACTCTTCCATCAAGA
TGGAGGTTGGGATTGAGGACTGTCTGCACATTGAATTTGAGTACAATAAATCCAAATACCACTTGAAAGA
TGTCATTGTAGGGAAGATATACTTCCTGCTGGTGAGAATCAAAATCAAGCACATGGAGATAGACATCATC
AAGCGAGAAACGACGGGTACAGGCCCCAACGTGTACCATGAGAATGACACGATAGCCAAGTACGAGATCA
TGGACGGGGCACCAGTGCGAGGAGAGTCCATCCCGATCCGGCTCTTCCTGGCCGGGTATGAGCTCACGCC
CACCATGCGGGACATCAACAAGAAGTTCTCTGTGCGCTATTACCTCAACCTGGTGCTGATAGACGAGGAG
GAGCGGCGCTACTTCAAGCAGCAGGAAGTGGTGTTGTGGCGGAAGGGTGACATCGTACGGAAGAGCATGT
CCCACCAGGCGGCCATCGCCTCACAGCGCTTTGAGGGCACCACCTCCCTGGGTGAGGTGCGGACCCCCAG
CCAGCTGTCTGACAACAACTGCAGGCAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG210499 representing NM_052875
Red=Cloning site Green=Tags(s)

MSFFGFGQSVEVEILLNDAESRKRAEHKTEDGKKEKYFLFYDGETVSGKVSLALRNPNKRLEHQGIKIEF
IGQIELYYDRGNHHEFVSLVKDLARPGEITQSQAFDFEFTHVEKPYESYTGQNVKLRYFLRATISRRLND
VVKEMDIVVHTLSTYPELNSSIKMEVGIEDCLHIEFEYNKSKYHLKDVIVGKIYFLLVRIKIKHMEIDII
KRETTGTGPNVYHENDTIAKYEIMDGAPVRGESIPIRLFLAGYELTPTMRDINKKFSVRYYLNLVLIDEE
ERRYFKQQEVVLWRKGDIVRKSMSHQAAIASQRFEGTTSLGEVRTPSQLSDNNCRQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_052875
ORF Size 1008 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_052875.3, NP_443107.1
RefSeq Size 3685 bp
RefSeq ORF 1011 bp
Locus ID 112936
UniProt ID Q4G0F5
Cytogenetics 11q25
Domains Vps26
Summary Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5. May be involved in retrograde transport of SORT1 but not of IGF2R. Acts redundantly with VSP26A in SNX-27 mediated endocytic recycling of SLC2A1/GLUT1 (By similarity).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_052875" in other vectors (6)
SKU Description Size Price
RC210499 VPS26B (Myc-DDK-tagged)-Human vacuolar protein sorting 26 homolog B (S. pombe) (VPS26B) 10 ug
$457.00
RC210499L1 Lenti-ORF clone of VPS26B (Myc-DDK-tagged)-Human vacuolar protein sorting 26 homolog B (S. pombe) (VPS26B) 10 ug
$757.00
RC210499L2 Lenti-ORF clone of VPS26B (mGFP-tagged)-Human vacuolar protein sorting 26 homolog B (S. pombe) (VPS26B) 10 ug
$757.00
RC210499L3 Lenti-ORF clone of VPS26B (Myc-DDK-tagged)-Human vacuolar protein sorting 26 homolog B (S. pombe) (VPS26B) 10 ug
$757.00
RC210499L4 Lenti-ORF clone of VPS26B (mGFP-tagged)-Human vacuolar protein sorting 26 homolog B (S. pombe) (VPS26B) 10 ug
$757.00
SC120157 VPS26B (untagged)-Human vacuolar protein sorting 26 homolog B (S. pombe) (VPS26B) 10 ug
$457.00

Plasmid Map for RG210499

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG210499
Circular map for RG210499

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products