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ISLR (NM_005545) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG210329
ISLR (tGFP-tagged) - Human immunoglobulin superfamily containing leucine-rich repeat (ISLR), transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $657.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ISLR
Synonyms HsT17563; Meflin
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG210329 representing NM_005545
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGAGCTGCATCTGCTCTGGTGGGCGCTTCTCCTGGGCCTGGCTCAGGCCTGCCCTGAGCCCTGCG
ACTGTGGGGAAAAGTATGGCTTCCAGATCGCCGACTGTGCCTACCGCGACCTAGAATCCGTGCCGCCTGG
CTTCCCGGCCAATGTGACTACACTGAGCCTGTCAGCCAACCGGCTGCCAGGCTTGCCGGAGGGTGCCTTC
AGGGAGGTGCCCCTGCTGCAGTCGCTGTGGCTGGCACACAATGAGATCCGCACGGTGGCCGCCGGAGCCC
TGGCCTCTCTGAGCCATCTCAAGAGCCTGGACCTCAGCCACAATCTCATCTCTGACTTTGCCTGGAGCGA
CCTGCACAACCTCAGTGCCCTCCAATTGCTCAAGATGGACAGCAACGAGCTGACCTTCATCCCCCGCGAC
GCCTTCCGCAGCCTCCGTGCTCTGCGCTCGCTGCAACTCAACCACAACCGCTTGCACACATTGGCCGAGG
GCACCTTCACCCCGCTCACCGCGCTGTCCCACCTGCAGATCAACGAGAACCCCTTCGACTGCACCTGCGG
CATCGTGTGGCTCAAGACATGGGCCCTGACCACGGCCGTGTCCATCCCGGAGCAGGACAACATCGCCTGC
ACCTCACCCCATGTGCTCAAGGGTACACCGCTGAGCCGCCTGCCGCCACTGCCATGCTCGGCGCCCTCAG
TGCAGCTCAGCTACCAACCCAGCCAGGATGGTGCCGAGCTGCGGCCTGGTTTTGTGCTGGCACTGCACTG
TGATGTGGACGGGCAGCCGGCCCCTCAGCTTCACTGGCACATCCAGATACCCAGTGGCATTGTGGAGATC
ACCAGCCCCAACGTGGGCACTGATGGGCGTGCCCTGCCTGGCACCCCTGTGGCCAGCTCCCAGCCGCGCT
TCCAGGCCTTTGCCAATGGCAGCCTGCTTATCCCCGACTTTGGCAAGCTGGAGGAAGGCACCTACAGCTG
CCTGGCCACCAATGAGCTGGGCAGTGCTGAGAGCTCAGTGGACGTGGCACTGGCCACGCCCGGTGAGGGT
GGTGAGGACACACTGGGGCGCAGGTTCCATGGCAAAGCGGTTGAGGGAAAGGGCTGCTATACGGTTGACA
ACGAGGTGCAGCCATCAGGGCCGGAGGACAATGTGGTCATCATCTACCTCAGCCGTGCTGGGAACCCTGA
AGCTGCAGTCGCAGAAGGGGTCCCTGGGCAGCTGCCCCCAGGCCTGCTCCTGCTGGGCCAAAGCCTCCTC
CTCTTCTTCTTCCTCACCTCCTTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG210329 representing NM_005545
Red=Cloning site Green=Tags(s)

MQELHLLWWALLLGLAQACPEPCDCGEKYGFQIADCAYRDLESVPPGFPANVTTLSLSANRLPGLPEGAF
REVPLLQSLWLAHNEIRTVAAGALASLSHLKSLDLSHNLISDFAWSDLHNLSALQLLKMDSNELTFIPRD
AFRSLRALRSLQLNHNRLHTLAEGTFTPLTALSHLQINENPFDCTCGIVWLKTWALTTAVSIPEQDNIAC
TSPHVLKGTPLSRLPPLPCSAPSVQLSYQPSQDGAELRPGFVLALHCDVDGQPAPQLHWHIQIPSGIVEI
TSPNVGTDGRALPGTPVASSQPRFQAFANGSLLIPDFGKLEEGTYSCLATNELGSAESSVDVALATPGEG
GEDTLGRRFHGKAVEGKGCYTVDNEVQPSGPEDNVVIIYLSRAGNPEAAVAEGVPGQLPPGLLLLGQSLL
LFFFLTSF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005545
ORF Size 1284 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005545.3, NP_005536.1
RefSeq Size 2312 bp
RefSeq ORF 1287 bp
Locus ID 3671
UniProt ID O14498
Cytogenetics 15q24.1
Domains IGc2, LRR, LRRCT, LRR_PS, LRR_TYP
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Documents
Resources
"NM_005545" in other vectors (4)
SKU Description Size Price
RC210329 ISLR (Myc-DDK-tagged)-Human immunoglobulin superfamily containing leucine-rich repeat (ISLR), transcript variant 1 10 ug
$457.00
RC210329L3 Lenti ORF clone of Human immunoglobulin superfamily containing leucine-rich repeat (ISLR), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC210329L4 Lenti ORF clone of Human immunoglobulin superfamily containing leucine-rich repeat (ISLR), transcript variant 1, mGFP tagged 10 ug
$757.00
SC116678 ISLR (untagged)-Human immunoglobulin superfamily containing leucine-rich repeat (ISLR), transcript variant 1 10 ug
$457.00

Plasmid Map for RG210329

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG210329
Circular map for RG210329

file-alt Citations

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