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IER2 (NM_004907) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG209947
IER2 (tGFP-tagged) - Human immediate early response 2 (IER2)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $500.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol IER2
Synonyms ETR101
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG209947 representing NM_004907
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGTGCAGAAAGAGGCACAGCGCATCATGACCCTGTCGGTGTGGAAGATGTATCACTCCCGCATGC
AGCGCGGTGGCCTGCGGCTGCACCGGAGTCTGCAGCTGTCGCTGGTCATGCGCAGCGCCCGGGAGCTCTA
CCTCTCGGCCAAGGTGGAGGCCCTCGAGCCCGAGGTGTCGTTGCCGGCCGCCCTCCCCTCTGACCCTCGC
CTGCACCCGCCCCGAGAAGCCGAGTCCACGGCCGAGACAGCGACCCCCGACGGTGAGCACCCGTTTCCGG
AGCCAATGGACACGCAGGAGGCGCCGACAGCCGAGGAGACCTCCGCCTGCTGTGCCCCGCGCCCCGCCAA
AGTCAGCCGCAAACGACGCAGCAGCAGCCTGAGCGACGGCGGGGACGCTGGACTGGTCCCGAGCAAGAAA
GCCCGTCTGGAAGAAAAGGAAGAAGAGGAGGGAGCGTCATCCGAAGTCGCCGATCGCCTGCAGCCCCCTC
CGGCGCAAGCGGAGGGCGCCTTTCCCAACCTGGCCCGCGTCCTGCAGAGGCGCTTCTCCGGCCTCCTGAA
CTGCAGCCCCGCGGCCCCTCCGACGGCGCCGCCCGCGTGCGAGGCAAAGCCCGCTTGCCGCCCGGCGGAC
AGCATGCTCAACGTGCTCGTGCGGGCCGTGGTGGCCTTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG209947 representing NM_004907
Red=Cloning site Green=Tags(s)

MEVQKEAQRIMTLSVWKMYHSRMQRGGLRLHRSLQLSLVMRSARELYLSAKVEALEPEVSLPAALPSDPR
LHPPREAESTAETATPDGEHPFPEPMDTQEAPTAEETSACCAPRPAKVSRKRRSSSLSDGGDAGLVPSKK
ARLEEKEEEEGASSEVADRLQPPPAQAEGAFPNLARVLQRRFSGLLNCSPAAPPTAPPACEAKPACRPAD
SMLNVLVRAVVAF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004907
ORF Size 669 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004907.3
RefSeq Size 2088 bp
RefSeq ORF 672 bp
Locus ID 9592
UniProt ID Q9BTL4
Cytogenetics 19p13.13
Protein Families Transcription Factors
Summary DNA-binding protein that seems to act as a transcription factor (PubMed:19584537). Involved in the regulation of neuronal differentiation, acts upon JNK-signaling pathway activation and plays a role in neurite outgrowth in hippocampal cells (By similarity). May mediate with FIBP FGF-signaling in the establishment of laterality in the embryo (By similarity). Promotes cell motility, seems to stimulate tumor metastasis (PubMed:22120713).UniProtKB/Swiss-Prot Function
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"NM_004907" in other vectors (5)
SKU Description Size Price
RC209947 IER2 (Myc-DDK-tagged)-Human immediate early response 2 (IER2) 10 ug
$300.00
RC209947L3 Lenti ORF clone of Human immediate early response 2 (IER2), Myc-DDK-tagged 10 ug
$600.00
RC209947L4 Lenti ORF clone of Human immediate early response 2 (IER2), mGFP tagged 10 ug
$600.00
SC117093 IER2 (untagged)-Human immediate early response 2 (IER2) 10 ug
$300.00
SC322023 IER2 (untagged)-Human immediate early response 2 (IER2) 10 ug
$300.00

Plasmid Map for RG209947

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for RG209947
Circular map for RG209947

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