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NIPAL1 (NM_207330) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG209874
NIPAL1 (tGFP-tagged) - Human NIPA-like domain containing 1 (NIPAL1)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $657.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NIPAL1
Synonyms NPAL1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG209874 representing NM_207330
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGCACAGGTGAGGCTGCCGCCCGGAGAGCCCTGCCGAGAAGGATATGTGCTGTCTCTGGTCTGTC
CAAACTCCTCCCAGGCTTGGTGTGAGATCACAAATGTGTCACAGCTGCTGGCTTCTCCTGTGCTCTACAC
GGACCTGAATTACAGCATAAACAACTTGAGCATTTCAGCAAATGTAGAAAACAAATACAGTCTTTATGTG
GGCTTGGTACTGGCAGTAAGCTCAAGTATTTTTATTGGCTCCAGCTTCATACTGAAAAAGAAGGGCCTCT
TGCAACTGGCCAGCAAGGGCTTTACTAGAGCTGGACAAGGTGGACATTCTTACCTGAAGGAATGGCTCTG
GTGGGTAGGATTGCTGTCAATGGGAGCAGGAGAGGCTGCAAATTTTGCTGCTTATGCTTTTGCACCTGCC
ACCTTGGTCACCCCTCTGGGTGCTTTGAGTGTTCTCATAAGTGCAATATTATCTTCCTACTTTTTAAACG
AGCACTTGAACATTCATGGGAAAATAGGCTGCATATTAAGTATATTGGGGTCAACTGTGATGGTTATCCA
TGCCCCACAAGAAGAGGAAGTCACATCTTTGCATGAAATGGAAATGAAATTGAGAGACCCAGGGTTTATT
TCCTTTGCTGTGATCATAACTGTGATCTCCTTGGTGCTGATTTTGATTGTGGCTCCCAAGAAAGGACAGA
CCAATATATTGGTTTATATTTCAATCTGTTCCTTGATTGGAGCGTTTTCAGTTTCTTCTGTGAAAGGCCT
GGGAATTGCCATTAAGGAGCTGATAGAATGGAAGCCAGTTTACAAACATCCGCTGGTCTTTGTTTTGCTG
GCTGTACTTGTGCTTTCAGTAACTACACAGATTAACTATCTCAACAAGGCACTGGACACCTTTAATACCT
CTCTTGTGACACCCATTTATTATGTATTCTTCACATCCATGGTAGTGACTTGCTCTGCCATCTTATTCCA
AGAGTGGTATGGCATGACAGCTGGAGATATCATTGGGACCCTGAGTGGATTCTTCACTATTATCATTGGC
ATCTTCCTTCTACATGCTTTTAAAAATACTGACATTACTTGGAGTGAGCTTACATCCACTGCTAAGAAAG
AAGCCGTCTCTCTGAATGTCAATGAAAACAATTATGTTTTACTAGAGAACTTGGAGTGTTCAGCCCCAGG
ATACAATGATGACGTTACCTTGTTTAGTAGAACTGATGAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG209874 representing NM_207330
Red=Cloning site Green=Tags(s)

MGAQVRLPPGEPCREGYVLSLVCPNSSQAWCEITNVSQLLASPVLYTDLNYSINNLSISANVENKYSLYV
GLVLAVSSSIFIGSSFILKKKGLLQLASKGFTRAGQGGHSYLKEWLWWVGLLSMGAGEAANFAAYAFAPA
TLVTPLGALSVLISAILSSYFLNEHLNIHGKIGCILSILGSTVMVIHAPQEEEVTSLHEMEMKLRDPGFI
SFAVIITVISLVLILIVAPKKGQTNILVYISICSLIGAFSVSSVKGLGIAIKELIEWKPVYKHPLVFVLL
AVLVLSVTTQINYLNKALDTFNTSLVTPIYYVFFTSMVVTCSAILFQEWYGMTAGDIIGTLSGFFTIIIG
IFLLHAFKNTDITWSELTSTAKKEAVSLNVNENNYVLLENLECSAPGYNDDVTLFSRTDD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_207330
ORF Size 1230 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_207330.3
RefSeq Size 2204 bp
RefSeq ORF 1233 bp
Locus ID 152519
UniProt ID Q6NVV3
Cytogenetics 4p12
Protein Families Transmembrane
Summary Acts as a Mg(2+) transporter. Can also transport other divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+), Cu(2+) and Co(2+) but to a much less extent than Mg(2+) (By similarity).UniProtKB/Swiss-Prot Function
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"NM_207330" in other vectors (4)
SKU Description Size Price
RC209874 NIPAL1 (Myc-DDK-tagged)-Human NIPA-like domain containing 1 (NIPAL1) 10 ug
$289.00 $457.00
RC209874L3 Lenti ORF clone of Human NIPA-like domain containing 1 (NIPAL1), Myc-DDK-tagged 10 ug
$757.00
RC209874L4 Lenti ORF clone of Human NIPA-like domain containing 1 (NIPAL1), mGFP tagged 10 ug
$757.00
SC308453 NIPAL1 (untagged)-Human NIPA-like domain containing 1 (NIPAL1) 10 ug
$503.00

Plasmid Map for RG209874

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG209874
Circular map for RG209874

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