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GPR4 (NM_005282) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG209686
GPR4 (tGFP-tagged) - Human G protein-coupled receptor 4 (GPR4)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $657.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GPR4
Synonyms GPR6C.l
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG209686 representing NM_005282
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAACCACACGTGGGAGGGCTGCCACGTGGACTCGCGCGTGGACCACCTCTTTCCGCCATCCCTCT
ACATCTTTGTCATCGGCGTGGGGCTGCCCACCAACTGCCTGGCTCTGTGGGCGGCCTACCGCCAGGTGCA
ACAGCGCAACGAGCTGGGCGTCTACCTGATGAACCTCAGCATCGCCGACCTGCTGTACATCTGCACGCTG
CCGCTGTGGGTGGACTACTTCCTGCACCACGACAACTGGATCCACGGCCCCGGGTCCTGCAAGCTCTTTG
GGTTCATCTTCTACACCAATATCTACATCAGCATCGCCTTCCTGTGCTGCATCTCGGTGGACCGCTACCT
GGCTGTGGCCCACCCACTCCGCTTCGCCCGCCTGCGCTGCGTCAAGACCGCCGTGGCCGTGAGCTCCGTG
GTCTGGGCCACGGAGCTGGGCGCCAACTCGGCGCCCCTGTTCCATGACGAGCTCTTCCGAGACCGCTACA
ACCACACCTTCTGCTTTGAGAAGTTCCCCATGGAAGGCTGGGTGGCCTGGATGAACCTCTATCGGGTGTT
CGTGGGCTTCCTCTTCCCGTGGGCGCTCATGCTGCTGTCGTACCGGGGCATCCTGCGGGCCGTGCGGGGC
AGCGTGTCCACCGAGCGCCAGGAGAAGGCCAAGATCAAGCGGCTGGCCCTCAGCCTCATCGCCATCGTGC
TGGTCTGCTTTGCGCCCTATCACGTGCTCTTGCTGTCCCGCAGCGCCATCTACCTGGGCCGCCCCTGGGA
CTGCGGCTTCGAGGAGCGCGTCTTTTCTGCATACCACAGCTCACTGGCTTTCACCAGCCTCAACTGTGTG
GCGGACCCCATCCTCTACTGCCTGGTCAACGAGGGCGCCCGCAGCGATGTGGCCAAGGCCCTGCACAACC
TGCTCCGCTTTCTGGCCAGCGACAAGCCCCAGGAGATGGCCAATGCCTCGCTCACCCTGGAGACCCCACT
CACCTCCAAGAGGAACAGCACAGCCAAAGCCATGACTGGCAGCTGGGCGGCCACTCCGCCCTCCCAGGGG
GACCAGGTGCAGCTGAAGATGCTGCCGCCAGCACAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG209686 representing NM_005282
Red=Cloning site Green=Tags(s)

MGNHTWEGCHVDSRVDHLFPPSLYIFVIGVGLPTNCLALWAAYRQVQQRNELGVYLMNLSIADLLYICTL
PLWVDYFLHHDNWIHGPGSCKLFGFIFYTNIYISIAFLCCISVDRYLAVAHPLRFARLRCVKTAVAVSSV
VWATELGANSAPLFHDELFRDRYNHTFCFEKFPMEGWVAWMNLYRVFVGFLFPWALMLLSYRGILRAVRG
SVSTERQEKAKIKRLALSLIAIVLVCFAPYHVLLLSRSAIYLGRPWDCGFEERVFSAYHSSLAFTSLNCV
ADPILYCLVNEGARSDVAKALHNLLRFLASDKPQEMANASLTLETPLTSKRNSTAKAMTGSWAATPPSQG
DQVQLKMLPPAQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005282
ORF Size 1086 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005282.1, NP_005273.1
RefSeq Size 2696 bp
RefSeq ORF 1089 bp
Locus ID 2828
UniProt ID P46093
Cytogenetics 19q13.32
Domains 7tm_1
Protein Families Druggable Genome, GPCR, Transmembrane
Summary Proton-sensing receptor coupled to several G-proteins, including G(s), G(13) and G(q)/G(11) proteins, leading to cAMP production.UniProtKB/Swiss-Prot Function
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"NM_005282" in other vectors (6)
SKU Description Size Price
RC209686 GPR4 (Myc-DDK-tagged)-Human G protein-coupled receptor 4 (GPR4) 10 ug
$457.00
RC209686L1 Lenti ORF clone of Human G protein-coupled receptor 4 (GPR4), Myc-DDK-tagged 10 ug
$757.00
RC209686L2 Lenti ORF clone of Human G protein-coupled receptor 4 (GPR4), mGFP tagged 10 ug
$757.00
RC209686L3 Lenti ORF clone of Human G protein-coupled receptor 4 (GPR4), Myc-DDK-tagged 10 ug
$757.00
RC209686L4 Lenti ORF clone of Human G protein-coupled receptor 4 (GPR4), mGFP tagged 10 ug
$757.00
SC116837 GPR4 (untagged)-Human G protein-coupled receptor 4 (GPR4) 10 ug
$457.00

Plasmid Map for RG209686

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG209686
Circular map for RG209686

file-alt Citations

GPR4 in the pH-dependent migration of melanoma cells in the tumor microenvironment
Stolwijk, Wallner, Heider et al
Exp Dermatol (2022)
Cited by 19
AI Summary: OriGene’s pCMV6-AC-GFP-based Human GPR4 Tagged ORF Clone (RG209686) was used to overexpress GPR4 in SK-Mel-28 melanoma cells, enabling the study of GPR4’s role in pH-dependent cell migration in the tumor microenvironment.
Snippet: with the pCMV6-AC-GFP-based expression plasmid GPR4 (NM_005282) Human Tagged ORF Clone (OriGene technologies, RG209686). Cells were selected by 1 mg/ml Neomycin (G418). SK-Mel-28 wildtype (WT) cells of similar passage number as GPR4 overexpressing cells served as control. An effect of transfection with
Showing 1-1 of 1 papers.
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