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PMF1 (NM_007221) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG209547
PMF1 (tGFP-tagged) - Human polyamine-modulated factor 1 (PMF1), transcript variant 2
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $500.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "PMF1 or NM_007221"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PMF1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG209547 representing NM_007221
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAAGCAAGTAGCGCCAATCTAGGCAGCGGCTGTGAGGAAAAAAGGCATGAGGGGTCGTCTTCGG
AATCTGTGCCACCCGGCACTACCATTTCGAGGGTGAAGCTCCTCGACACCATGGTGGACACTTTTCTTCA
GAAGCTGGTCGCCGCCGGCAGCTACCAGAGATTCACTGACTGCTATAAGTGCTTCTACCAGTTGCAGCCT
GCGATGACACAGCGAATCTATGACAAGTTTATAGCTCAGTTGCAGACATCTATCCGGGAGGAAATCTCTG
ACATCAAAGAGGAGGGGAACCTAGAAGCTGTCTTGAATGCCTTGGATAAAATTGTGGAAGAAGGCAAAGT
CCGCAAAGAGCCAGCCTGGCGCCCCAGCGGGATCCCAGAGAAGGATCTGCACAGTGTTATAGCACCCTAC
TTCCTGCAGCAACGGGACACCCTGCGGCGCCATGTGCAGAAACAGGAGGCCGAGAACCAGCAGCTGGCAG
ATGCCGTCCTGGCAGGGCGGAGGCAGGTGGAGGAGCTGCAGCTACAGGTCCAGGCCCAGCAGCAGGCCTG
GCAGGCTCTACACAGAGAACAGAGGGAGCTGGTTGCTGTGCTGAGGGAGCCTGAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG209547 representing NM_007221
Red=Cloning site Green=Tags(s)

MAEASSANLGSGCEEKRHEGSSSESVPPGTTISRVKLLDTMVDTFLQKLVAAGSYQRFTDCYKCFYQLQP
AMTQRIYDKFIAQLQTSIREEISDIKEEGNLEAVLNALDKIVEEGKVRKEPAWRPSGIPEKDLHSVIAPY
FLQQRDTLRRHVQKQEAENQQLADAVLAGRRQVEELQLQVQAQQQAWQALHREQRELVAVLREPE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007221
ORF Size 615 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007221.2, NP_009152.2
RefSeq Size 1082 bp
RefSeq ORF 618 bp
Locus ID 11243
UniProt ID Q6P1K2
Cytogenetics 1q22
Protein Families Stem cell - Pluripotency
Summary Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.UniProtKB/Swiss-Prot Function
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"NM_007221" in other vectors (5)
SKU Description Size Price
RC209547 PMF1 (Myc-DDK-tagged)-Human polyamine-modulated factor 1 (PMF1), transcript variant 2 10 ug
$300.00
RC209547L3 Lenti ORF clone of Human polyamine-modulated factor 1 (PMF1), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC209547L4 Lenti ORF clone of Human polyamine-modulated factor 1 (PMF1), transcript variant 2, mGFP tagged 10 ug
$600.00
SC108493 PMF1 (untagged)-Human polyamine-modulated factor 1 (PMF1), transcript variant 2 10 ug
$150.00
SC317305 PMF1 (untagged)-Human polyamine-modulated factor 1 (PMF1), transcript variant 2 10 ug
$330.00

Plasmid Map for RG209547

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG209547
Circular map for RG209547

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