MASA (ENOPH1) (NM_021204) Human Tagged ORF Clone

  • TrueORF®
SKU
RG209510
ENOPH1 (tGFP-tagged) - Human enolase-phosphatase 1 (ENOPH1)
$489.00 $500.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MASA
Synonyms E1; MASA; MST145; mtnC
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG209510 representing NM_021204
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCGTGCTTTCGGTCCCCGCCGAAGTCACCGTGATCCTGTTAGATATCGAAGGTACCACAACCCCGA
TTGCTTTCGTGAAGGACATTTTATTTCCTTACATCGAAGAAAATGTTAAAGAGTATCTGCAGACACATTG
GGAAGAAGAGGAGTGCCAGCAGGATGTCAGTCTTTTGAGGAAACAGGCTGAAGAGGACGCCCACCTGGAT
GGGGCTGTTCCTATCCCTGCAGCATCTGGGAATGGAGTGGATGATCTGCAACAGATGATCCAGGCCGTGG
TAGATAATGTGTGCTGGCAGATGTCCCTGGATCGAAAGACCACTGCACTCAAACAGCTGCAGGGCCACAT
GTGGAGGGCGGCATTCACAGCTGGGCGCATGAAAGCAGAGTTCTTTGCAGATGTAGTTCCAGCAGTCAGG
AAGTGGAGAGAGGCCGGAATGAAGGTGTACATCTATTCCTCAGGGAGTGTGGAGGCACAGAAACTGTTAT
TCGGGCATTCTACGGAGGGAGATATTCTTGAGCTTGTTGATGGTCACTTTGATACCAAGATTGGACACAA
AGTAGAGAGTGAAAGTTACCGAAAGATTGCAGACAGCATTGGGTGCTCAACCAACAACATTTTGTTTCTG
ACAGATGTTACTCGAGAGGCCAGTGCTGCTGAGGAAGCAGATGTGCACGTAGCTGTGGTGGTGAGACCAG
GCAACGCAGGATTAACAGATGATGAGAAGACTTACTACAGCCTCATCACATCCTTCAGTGAACTATACCT
GCCTTCCTCAACC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG209510 representing NM_021204
Red=Cloning site Green=Tags(s)

MVVLSVPAEVTVILLDIEGTTTPIAFVKDILFPYIEENVKEYLQTHWEEEECQQDVSLLRKQAEEDAHLD
GAVPIPAASGNGVDDLQQMIQAVVDNVCWQMSLDRKTTALKQLQGHMWRAAFTAGRMKAEFFADVVPAVR
KWREAGMKVYIYSSGSVEAQKLLFGHSTEGDILELVDGHFDTKIGHKVESESYRKIADSIGCSTNNILFL
TDVTREASAAEEADVHVAVVVRPGNAGLTDDEKTYYSLITSFSELYLPSST

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021204
ORF Size 783 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021204.5
RefSeq Size 1992 bp
RefSeq ORF 786 bp
Locus ID 58478
UniProt ID Q9UHY7
Cytogenetics 4q21.22
Domains Hydrolase
Protein Pathways Cysteine and methionine metabolism
Summary Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_021204" in other vectors (5)
SKU Description Size Price
RC209510 ENOPH1 (Myc-DDK-tagged)-Human enolase-phosphatase 1 (ENOPH1) 10 ug
$300.00
RC209510L3 Lenti ORF clone of Human enolase-phosphatase 1 (ENOPH1), Myc-DDK-tagged 10 ug
$600.00
RC209510L4 Lenti ORF clone of Human enolase-phosphatase 1 (ENOPH1), mGFP tagged 10 ug
$600.00
SC112927 ENOPH1 (untagged)-Human enolase-phosphatase 1 (ENOPH1) 10 ug
$300.00
SC321962 ENOPH1 (untagged)-Human enolase-phosphatase 1 (ENOPH1) 10 ug
$300.00

Plasmid Map for RG209510

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Cloning scheme for RG209510

Circular map for RG209510

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