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SNAP-beta (NAPB) (NM_022080) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG209278
NAPB (tGFP-tagged) - Human N-ethylmaleimide-sensitive factor attachment protein, beta (NAPB)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $500.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SNAP-beta
Synonyms SNAP-BETA; SNAPB
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG209278 representing NM_022080
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACAACGCGGGGAAGGAGCGTGAGGCAGTACAGCTGATGGCGGAGGCCGAGAAGCGAGTCAAGGCCT
CCCACTCCTTCCTCCGAGGGCTGTTTGGAGGAAACACAAGAATAGAAGAGGCTTGTGAAATGTATACCAG
AGCTGCAAATATGTTCAAGATGGCTAAAAATTGGAGTGCTGCAGGAAACGCATTTTGTCAGGCAGCCAAG
CTCCACATGCAGCTTCAGAGCAAACATGACTCTGCTACCAGCTTTGTGGATGCTGGAAATGCTTACAAAA
AGGCAGATCCCCAAGAGGCTATCAACTGCTTAAATGCAGCCATCGACATTTACACAGACATGGGAAGGTT
TACAATTGCAGCCAAGCACCACATTACTATTGCAGAGATCTATGAGACTGAACTTGTAGACATTGAGAAG
GCTATTGCACATTATGAACAATCTGCTGATTATTACAAAGGAGAAGAATCCAACAGCTCAGCAAACAAGT
GTCTGCTGAAGGTGGCAGCATATGCTGCCCAGCTTGAGCAGTACCAGAAAGCCATTGAGATCTATGAGCA
GGTTGGGGCAAACACAATGGATAATCCTTTGTTGAAATACAGTGCAAAGGATTACTTCTTCAAAGCTGCC
CTCTGCCACTTCATAGTAGACGAGTTGAATGCCAAGCTTGCTCTTGAGAAATATGAGGAAATGTTTCCAG
CATTTACTGATTCAAGAGAATGTAAATTATTGAAAAAACTCCTAGAAGCTCATGAAGAACAGAACAGTGA
AGCTTACACTGAAGCAGTGAAGGAATTTGACTCAATATCTCGCTTGGATCAGTGGCTGACCACCATGTTG
CTTCGCATCAAAAAGTCCATCCAAGGGGATGGAGAAGGAGATGGAGACCTAAAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG209278 representing NM_022080
Red=Cloning site Green=Tags(s)

MDNAGKEREAVQLMAEAEKRVKASHSFLRGLFGGNTRIEEACEMYTRAANMFKMAKNWSAAGNAFCQAAK
LHMQLQSKHDSATSFVDAGNAYKKADPQEAINCLNAAIDIYTDMGRFTIAAKHHITIAEIYETELVDIEK
AIAHYEQSADYYKGEESNSSANKCLLKVAAYAAQLEQYQKAIEIYEQVGANTMDNPLLKYSAKDYFFKAA
LCHFIVDELNAKLALEKYEEMFPAFTDSRECKLLKKLLEAHEEQNSEAYTEAVKEFDSISRLDQWLTTML
LRIKKSIQGDGEGDGDLK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022080
ORF Size 894 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022080.3
RefSeq Size 3798 bp
RefSeq ORF 897 bp
Locus ID 63908
UniProt ID Q9H115
Cytogenetics 20p11.21
Summary This gene encodes a member of the soluble N-ethyl-maleimide-sensitive fusion attachment protein (SNAP) family. SNAP proteins play a critical role in the docking and fusion of vesicles to target membranes as part of the 20S NSF-SNAP-SNARE complex. This gene encodes the SNAP beta isoform which has been shown to be preferentially expressed in brain tissue. The encoded protein also interacts with the GluR2 α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptor subunit C-terminus and may play a role as a chaperone in the molecular processing of the AMPA receptor. provided by RefSeq, Mar 2017
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Documents
Resources
"NM_022080" in other vectors (4)
SKU Description Size Price
RC209278 NAPB (Myc-DDK-tagged)-Human N-ethylmaleimide-sensitive factor attachment protein, beta (NAPB) 10 ug
$300.00
RC209278L3 Lenti ORF clone of Human N-ethylmaleimide-sensitive factor attachment protein, beta (NAPB), Myc-DDK-tagged 10 ug
$600.00
RC209278L4 Lenti ORF clone of Human N-ethylmaleimide-sensitive factor attachment protein, beta (NAPB), mGFP tagged 10 ug
$600.00
SC304988 NAPB (untagged)-Human N-ethylmaleimide-sensitive factor attachment protein, beta (NAPB) 10 ug
$330.00

Plasmid Map for RG209278

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file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG209278
Circular map for RG209278

file-alt Citations

Experimental validation of predicted subcellular localizations of human proteins
Chaturvedi, Mir, Band et al
BMC Res Notes (2014) 7 (1), 912 DOI: 10.1186/1756-0500-7-912
Cited by 5
AI Summary: OriGene contributed to this study by providing ten GFP-tagged full-length human gene constructs—SACM1L, ST13, TUBAL3, USMG5, DECR2, AMY2B, UXS1, LGMN, NR2F1, and NAPB—which were used for experimental validation of predicted protein subcellular localizations.
Snippet: Ten GFP-tagged full- length human gene constructs (ngLOC predicted), which include: SACM1L, ST13, TUBAL3, USMG5, DECR2, AMY2B, UXS1, LGMN, NR2F1 and NAPB were obtained from Origene Technologies Inc. (Rockville, MD). ... Cite SaveSaving
Showing 1-1 of 1 papers.
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