MMP14 (NM_004995) Human Tagged ORF Clone
- TrueORF®
SKU
RG208917
MMP14 (tGFP-tagged) - Human matrix metallopeptidase 14 (membrane-inserted) (MMP14)
$489.00
$1,012.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | MMP14 |
| Synonyms | MMP-14; MMP-X1; MT-MMP; MT-MMP 1; MT1-MMP; MT1MMP; MTMMP1; WNCHRS |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004995 |
| ORF Size | 1746 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004995.2, NP_004986.1 |
| RefSeq Size | 3558 bp |
| RefSeq ORF | 1749 bp |
| Locus ID | 4323 |
| UniProt ID | P50281 |
| Cytogenetics | 14q11.2 |
| Domains | hemopexin, Peptidase_M10, ZnMc |
| Protein Families | Druggable Genome, Protease, Transmembrane |
| Protein Pathways | GnRH signaling pathway |
| Summary | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004995" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC208917 | MMP14 (Myc-DDK-tagged)-Human matrix metallopeptidase 14 (membrane-inserted) (MMP14) | 10 ug |
$812.00
|
|
| RC208917L1 | Lenti ORF clone of Human matrix metallopeptidase 14 (membrane-inserted) (MMP14), Myc-DDK-tagged | 10 ug |
$1,112.00
|
|
| RC208917L2 | Lenti ORF clone of Human matrix metallopeptidase 14 (membrane-inserted) (MMP14), mGFP tagged | 10 ug |
$1,112.00
|
|
| RC208917L3 | Lenti ORF clone of Human matrix metallopeptidase 14 (membrane-inserted) (MMP14), Myc-DDK-tagged | 10 ug |
$1,112.00
|
|
| RC208917L4 | Lenti ORF clone of Human matrix metallopeptidase 14 (membrane-inserted) (MMP14), mGFP tagged | 10 ug |
$1,112.00
|
|
| SC116990 | MMP14 (untagged)-Human matrix metallopeptidase 14 (membrane-inserted) (MMP14) | 10 ug |
$814.00
|
|
Citations
Loss of MT1-MMP in Alveolar Epithelial Cells Exacerbates Pulmonary Fibrosis
Placido, Romero, Maldonado et al
Int J Mol Sci (2021) 22 (6) DOI: 10.3390/ijms22062923
Placido, Romero, Maldonado et al
Int J Mol Sci (2021) 22 (6) DOI: 10.3390/ijms22062923
Cited by 33
AI Summary: OriGene products, specifically the MMP14 Human cDNA ORF Clone (Cat# RC209545) and MMP14 shRNA plasmid (Cat# TL313980), were used in this study to perform gain- and loss-of-function experiments in human A549 epithelial cells, helping to elucidate the role of MMP14 in regulating cellular senescence and fibrosis-related signaling.
Loss of MT1-MMP in Alveolar Epithelial Cells Exacerbates Pulmonary Fibrosis
Placido, Romero, Maldonado et al
IJMS (2021) 22 (6), 2923 DOI: 10.3390/ijms22062923
Placido, Romero, Maldonado et al
IJMS (2021) 22 (6), 2923 DOI: 10.3390/ijms22062923
Cited by 33
AI Summary: OriGene’s full-length human MMP14 cDNA (pCMV6-AC-GFP, RG208917) was used to manipulate MMP14 expression in human epithelial cells, enabling the study of MMP14’s role in pulmonary fibrosis.
Snippet: Full-length human MMP14 cDNA cloned into the pCMV6-AC-GFP vector was obtained from Origene (RG208917). MMP14 was silenced by the overexpression of short hairpin RNA directed to MMP14
Showing 1-2 of 2 papers.
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