RAP1GAP (NM_002885) Human Tagged ORF Clone

SKU RG208271

RAP1GAP (tGFP-tagged) - Human RAP1 GTPase activating protein (RAP1GAP), transcript variant 3

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: RAP1GAP
• Synonyms: RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208271 representing NM_002885; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGATTGAGAAGATGCAGGGAAGCAGGATGGATGAACAACGCTGCTCCTTCCCGCCGCCCCTCAAAACAG; AGGAGGACTACATTCCATACCCGAGCGTGCACGAGGTCTTGGGGCGAGAAGGACCCTTCCCCCTCATCCT; GCTGCCCCAGTTTGGGGGCTACTGGATTGAGGGCACCAACCACGAAATCACCAGCATCCCCGAGACAGAG; CCACTGCAGTCGCCCACAACCAAGGTGAAGCTCGAGTGCAACCCCACAGCCCGCATCTACCGGAAGCACT; TTCTCGGCAAGGAGCATTTCAATTACTACTCACTGGACGCTGCCCTCGGCCACCTTGTCTTCTCACTCAA; GTACGATGTCATCGGGGACCAAGAGCACCTGCGGCTGCTGCTCAGGACCAAGTGCCGGACATACCATGAT; GTCATCCCCATCTCCTGCCTCACCGAGTTCCCTAATGTTGTCCAGATGGCAAAGTTGGTGTGTGAAGACG; TCAATGTGGATCGGTTCTATCCTGTGCTCTACCCCAAGGCTTCCCGGCTCATCGTCACCTTTGACGAGCA; TGTCATCAGCAATAACTTCAAGTTTGGCGTCATTTATCAGAAGCTTGGGCAGACCTCCGAGGAAGAACTC; TTCAGCACCAATGAGGAAAGTCCCGCTTTCGTGGAGTTCCTTGAATTTCTTGGCCAGAAGGTCAAACTGC; AGGACTTTAAGGGGTTCCGAGGAGGCCTGGACGTGACCCACGGGCAGACGGGGACCGAATCTGTGTACTG; CAACTTCCGCAACAAGGAGATCATGTTTCACGTGTCCACCAAGCTGCCATACACGGAAGGGGACGCCCAG; CAGTTGCAGCGGAAGCGGCACATCGGGAACGACATCGTGGCTGTGGTCTTCCAGGATGAGAACACTCCTC; TCGTGCCCGACATGATCGCGTCCAACTTCCTGCATGCCTACGTCGTGGTGCAGGCTGAGGGCGGGGGCCC; TGATGGCCCCCTCTACAAGGTCTCTGTCACTGCAAGAGATGATGTGCCCTTCTTTGGACCCCCCCTCCCG; GACCCCGCTGTGTTCAGGAAGGGGCCTGAGTTCCAGGAATTTTTGCTGACAAAGCTGATCAATGCTGAAT; ATGCCTGCTACAAGGCAGAGAAGTTTGCCAAACTGGAGGAGCGGACGCGGGCCGCCCTCCTGGAGACGCT; CTATGAGGAACTACACATCCACAGCCAGTCCATGATGGGCTTGGGCGGCGACGAGGACAAGATGGAGAAT; GGCAGTGGGGGCGGCGGCTTCTTTGAGTCTTTCAAGCGGGTCATCCGGAGCCGCAGCCAGTCCATGGATG; CCATGGGGCTGAGCAACAAGAAGCCCAACACCGTGTCCACCAGCCACAGCGGGAGCTTCGCGCCCAACAA; CCCCGACCTGGCCAAGGCGGCTGGAATATCACTGATTGTCCCTGGGAAGAGCCCCACGAGGAAGAAGTCG; GGCCCGTTCGGCTCCCGCCGCAGCAGCGCCATTGGCATCGAGAACATACAGGAGGTGCAGGAGAAGAGGG; AGAGCCCTCCGGCTGGTCAGAAGACCCCAGACAGCGGGCACGTCTCACAGGAGCCCAAGTCGGAGAACTC; ATCCACTCAGAGCTCCCCAGAGATGCCCACGACCAAGAACAGAGCGGAGACCGCAGCGCAGAGAGCAGAG; GCGCTCAAGGACTTCTCCCGCTCCTCGTCCAGTGCCAGCAGCTTCGCCAGCGTGGTGGAGGAGACGGAGG; GTGTGGACGGAGAGGACACAGGCCTGGAGAGCGTGTCATCCTCAGGAACACCCCACAAGCGGGACTCCTT; CATCTATAGCACGTGGCTGGAGGACAGTGTCAGCACCACTAGTGGGGGCAGCTCCCCAGGCCCCTCTCGA; TCACCCCACCCAGACGCCGGCAAGTTGGGGGACCCTGCGTGTCCCGAGATCAAGATCCAGCTGGAAGCAT; CTGAGCAGCACATGCCCCAGCTGGGCTGT; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208271 representing NM_002885; Red=Cloning site Green=Tags(s); ; MIEKMQGSRMDEQRCSFPPPLKTEEDYIPYPSVHEVLGREGPFPLILLPQFGGYWIEGTNHEITSIPETE; PLQSPTTKVKLECNPTARIYRKHFLGKEHFNYYSLDAALGHLVFSLKYDVIGDQEHLRLLLRTKCRTYHD; VIPISCLTEFPNVVQMAKLVCEDVNVDRFYPVLYPKASRLIVTFDEHVISNNFKFGVIYQKLGQTSEEEL; FSTNEESPAFVEFLEFLGQKVKLQDFKGFRGGLDVTHGQTGTESVYCNFRNKEIMFHVSTKLPYTEGDAQ; QLQRKRHIGNDIVAVVFQDENTPLVPDMIASNFLHAYVVVQAEGGGPDGPLYKVSVTARDDVPFFGPPLP; DPAVFRKGPEFQEFLLTKLINAEYACYKAEKFAKLEERTRAALLETLYEELHIHSQSMMGLGGDEDKMEN; GSGGGGFFESFKRVIRSRSQSMDAMGLSNKKPNTVSTSHSGSFAPNNPDLAKAAGISLIVPGKSPTRKKS; GPFGSRRSSAIGIENIQEVQEKRESPPAGQKTPDSGHVSQEPKSENSSTQSSPEMPTTKNRAETAAQRAE; ALKDFSRSSSSASSFASVVEETEGVDGEDTGLESVSSSGTPHKRDSFIYSTWLEDSVSTTSGGSSPGPSR; SPHPDAGKLGDPACPEIKIQLEASEQHMPQLGC; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002885
• ORF Size: 1989 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002885.1, NP_002876.1
• RefSeq Size: 3270 bp
• RefSeq ORF: 1992 bp
• Locus ID: 5909
• UniProt ID: P47736
• Cytogenetics: 1p36.12
• Summary: This gene encodes a type of GTPase-activating-protein (GAP) that down-regulates the activity of the ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an inactive GDP-bound form and an active GTP-bound form. The product of this gene, RAP1GAP, promotes the hydrolysis of bound GTP and hence returns RAP1 to the inactive state whereas other proteins, guanine nucleotide exchange factors (GEFs), act as RAP1 activators by facilitating the conversion of RAP1 from the GDP- to the GTP-bound form. In general, ras subfamily proteins, such as RAP1, play key roles in receptor-linked signaling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation, adhesion, differentiation, and embryogenesis. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Aug 2011]