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FOXG1 (NM_005249) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG207964
FOXG1 (tGFP-tagged) - Human forkhead box G1 (FOXG1)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
$489.00 $886.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FOXG1
Synonyms BF1; BF2; FHKL3; FKH2; FKHL1; FKHL2; FKHL3; FKHL4; FOXG1A; FOXG1B; FOXG1C; HBF-1; HBF-2; HBF-3; HBF-G2; HBF2; HFK1; HFK2; HFK3; KHL2; QIN
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG207964 representing NM_005249
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGGACATGGGAGATAGGAAAGAGGTGAAAATGATCCCCAAGTCCTCGTTCAGCATCAACAGCCTGG
TGCCCGAGGCGGTCCAGAACGACAACCACCACGCGAGCCACGGCCACCACAACAGCCACCACCCCCAGCA
CCACCACCACCACCACCACCATCACCACCACCCGCCGCCGCCCGCCCCGCAACCGCCGCCGCCGCCGCAG
CAGCAGCAGCCGCCGCCGCCGCCGCCCCCGGCACCGCAGCCCCCCCAGACGCGGGGCGCCCCGGCCGCCG
ACGACGACAAGGGCCCCCAGCAGCTGCTGCTCCCGCCGCCGCCACCGCCACCACCGGCCGCCGCCCTGGA
CGGGGCTAAAGCGGTCGGGCTGGGCGGCAAGGGCGAGCCGGGCGGCGGGCCGGGGGAGCTGGCGCCCGTC
GGGCCGGACGAGAAGGAGAAGGGCGCCGGCGCCGGGGGGGAGGAGAAGAAGGGGGCGGGCGAGGGCGGCA
AGGACGGGGAGGGGGGCAAGGAGGGCGAGAAGAAGAACGGCAAGTACGAGAAGCCGCCGTTCAGCTACAA
CGCGCTCATCATGATGGCCATCCGGCAGAGCCCCGAGAAGCGGCTCACGCTCAACGGCATCTACGAGTTC
ATCATGAAGAACTTCCCTTACTACCGCGAGAACAAGCAGGGCTGGCAGAACTCCATCCGCCACAATCTGT
CCCTCAACATGTGCTTCGTGAAGGTGCCGCGCCACTACGACGACCCGGGCAAGGGCAACTACTGGATGCT
GGACCCGTCGAGCGACGACGTGTTCATCGGCGGCACCACGGGCAAGCTGCGGCGCCGCTCCACCACCTCG
CGGGCCAAGCTGGCCTTCAAGCGCGGTGCGCGCCTCACCTCCACCGGCCTCACCTTCATGGACCGCGCCG
GCTCCCTCTACTGGCCCATGTCGCCCTTCCTGTCCCTGCACCACCCCCGCGCCAGCAGCACTTTGAGTTA
CAACGGCACCACGTCGGCCTACCCCAGCCACCCCATGCCCTACAGCTCCGTGTTGACTCAGAACTCGCTG
GGCAACAACCACTCCTTCTCCACCGCCAACGGCCTGAGCGTGGACCGGCTGGTCAACGGGGAGATCCCGT
ACGCCACGCACCACCTCACGGCCGCCGCGCTAGCCGCCTCGGTGCCCTGCGGCCTGTCGGTGCCCTGCTC
TGGGACCTACTCCCTCAACCCCTGCTCCGTCAACCTGCTCGCGGGCCAGACCAGTTACTTTTTCCCCCAC
GTCCCGCACCCGTCAATGACTTCGCAGAGCAGCACGTCCATGAGCGCCAGGGCCACGTCCTCCTCCACGT
CGCCGCAGGCCCCCTCGACCCTGCCCTGTGAGTCTTTAAGACCCTCTTTGCCAAGTTTTACGACGGGACT
GTCTGGGGGACTGTCTGATTATTTCACACATCAAAATCAGGGGTCTTCTTCCAACCCTTTAATACAT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG207964 representing NM_005249
Red=Cloning site Green=Tags(s)

MLDMGDRKEVKMIPKSSFSINSLVPEAVQNDNHHASHGHHNSHHPQHHHHHHHHHHHPPPPAPQPPPPPQ
QQQPPPPPPPAPQPPQTRGAPAADDDKGPQQLLLPPPPPPPPAAALDGAKAVGLGGKGEPGGGPGELAPV
GPDEKEKGAGAGGEEKKGAGEGGKDGEGGKEGEKKNGKYEKPPFSYNALIMMAIRQSPEKRLTLNGIYEF
IMKNFPYYRENKQGWQNSIRHNLSLNMCFVKVPRHYDDPGKGNYWMLDPSSDDVFIGGTTGKLRRRSTTS
RAKLAFKRGARLTSTGLTFMDRAGSLYWPMSPFLSLHHPRASSTLSYNGTTSAYPSHPMPYSSVLTQNSL
GNNHSFSTANGLSVDRLVNGEIPYATHHLTAAALAASVPCGLSVPCSGTYSLNPCSVNLLAGQTSYFFPH
VPHPSMTSQSSTSMSARATSSSTSPQAPSTLPCESLRPSLPSFTTGLSGGLSDYFTHQNQGSSSNPLIH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005249
ORF Size 1467 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005249.3, NP_005240.3
RefSeq Size 2600 bp
RefSeq ORF 1470 bp
Locus ID 2290
UniProt ID P55316
Cytogenetics 14q12
Domains FH
Protein Families Druggable Genome, Transcription Factors
Summary This locus encodes a member of the fork-head transcription factor family. The encoded protein, which functions as a transcriptional repressor, is highly expressed in neural tissues during brain development. Mutations at this locus have been associated with Rett syndrome and a diverse spectrum of neurodevelopmental disorders defined as part of the FOXG1 syndrome. This gene is disregulated in many types of cancer and is the target of multiple microRNAs that regulate the proliferation of tumor cells. provided by RefSeq, Jul 2020
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Documents
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"NM_005249" in other vectors (6)
SKU Description Size Price
RC207964 FOXG1 (Myc-DDK-tagged)-Human forkhead box G1 (FOXG1) 10 ug
$686.00
RC207964L1 Lenti ORF clone of Human forkhead box G1 (FOXG1), Myc-DDK-tagged 10 ug
$986.00
RC207964L2 Lenti ORF clone of Human forkhead box G1 (FOXG1), mGFP tagged 10 ug
$986.00
RC207964L3 Lenti ORF clone of Human forkhead box G1 (FOXG1), Myc-DDK-tagged 10 ug
$986.00
RC207964L4 Lenti ORF clone of Human forkhead box G1 (FOXG1), mGFP tagged 10 ug
$986.00
SC116871 FOXG1 (untagged)-Human forkhead box G1 (FOXG1) 10 ug
$686.00

Plasmid Map for RG207964

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG207964
Circular map for RG207964

file-alt Citations

Zika virus induces FOXG1 nuclear displacement and downregulation in human neural progenitors
Lottini, Baggiani, Chesi et al
Stem Cell Reports (2022) 17 (7), 1683-1698 DOI: 10.1016/j.stemcr.2022.05.008
Cited by 24
AI Summary: OriGene contributed to this study by providing the plasmid encoding human FOXG1 fused to GFP (Cat: RG207964), which was used to investigate the effects of Zika virus infection on FOXG1 localization and expression in human neural progenitor cells.
Snippet: d to the N- and C- termini (GFP-FOXG1 and FOXG1-GFP wt) and 234-391-GFP fragments were previously described (Pancrazi et al., 2015). Plasmids encoding for human FOXG1 fused to GFP was purchased from Origene (Cat: RG207964). Constructs encoding for the N- and C- parts of FOXG1 (1–280 and 280–481 respectively) fused to the GFP at N-terminus, were purchased at IDT, Belgium. Immunofluorescence T
Showing 1-1 of 1 papers.
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