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DNAJB8 (NM_153330) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG207352
DNAJB8 (tGFP-tagged) - Human DnaJ (Hsp40) homolog, subfamily B, member 8 (DNAJB8)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DNAJB8
Synonyms CT156; DJ6
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG207352 representing NM_153330
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTAACTACTACGAAGTGCTGGGCGTGCAGGCCAGCGCTTCCCCGGAGGACATCAAGAAAGCCTACC
GCAAGCTGGCCCTTCGTTGGCACCCCGACAAGAACCCTGACAATAAGGAGGAGGCGGAGAAGAAGTTCAA
GCTGGTGTCTGAGGCCTATGAGGTTCTGTCTGACTCCAAGAAACGCTCCCTGTATGACCGTGCTGGCTGT
GACAGCTGGCGGGCTGGTGGCGGGGCCAGCACGCCCTACCACAGCCCCTTCGACACCGGCTACACCTTCC
GTAACCCTGAGGACATCTTCCGGGAGTTTTTCGGTGGCCTGGACCCTTTCTCCTTTGAGTTCTGGGATAG
CCCATTCAATAGTGACCGTGGTGGCCGGGGCCATGGCCTGAGGGGGGCCTTCTCGGCAGGCTTTGGAGAA
TTTCCGGCCTTCATGGAGGCCTTCTCATCCTTCAACATGCTGGGCTGCAGCGGGGGCAGCCACACCACCT
TCTCATCCACCTCCTTCGGGGGCTCCAGTTCTGGCAGCTCGGGGTTCAAGTCGGTGATGTCGTCCACCGA
GATGATCAATGGCCACAAGGTCACCACCAAGCGCATCGTGGAGAACGGGCAGGAGCGCGTGGAGGTGGAG
GAAGACGGGCAGCTCAAGTCGGTGACTGTGAACGGCAAGGAGCAGCTCAAATGGATGGACAGCAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG207352 representing NM_153330
Red=Cloning site Green=Tags(s)

MANYYEVLGVQASASPEDIKKAYRKLALRWHPDKNPDNKEEAEKKFKLVSEAYEVLSDSKKRSLYDRAGC
DSWRAGGGASTPYHSPFDTGYTFRNPEDIFREFFGGLDPFSFEFWDSPFNSDRGGRGHGLRGAFSAGFGE
FPAFMEAFSSFNMLGCSGGSHTTFSSTSFGGSSSGSSGFKSVMSSTEMINGHKVTTKRIVENGQERVEVE
EDGQLKSVTVNGKEQLKWMDSK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153330
ORF Size 696 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153330.2, NP_699161.1
RefSeq Size 2479 bp
RefSeq ORF 699 bp
Locus ID 165721
UniProt ID Q8NHS0
Cytogenetics 3q21.3
Summary The protein encoded by this gene belongs to the DNAJ/HSP40 family of proteins that regulate chaperone activity. This family member suppresses aggregation and toxicity of polyglutamine proteins, and the C-terminal tail is essential for this activity. It has been implicated as a cancer-testis antigen and as a cancer stem-like cell antigen involved in renal cell carcinoma. provided by RefSeq, Jun 2012
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"NM_153330" in other vectors (4)
SKU Description Size Price
RC207352 DNAJB8 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily B, member 8 (DNAJB8) 10 ug
$289.00 $300.00
RC207352L3 Lenti-ORF clone of DNAJB8 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily B, member 8 (DNAJB8) 10 ug
$600.00
RC207352L4 Lenti-ORF clone of DNAJB8 (mGFP-tagged)-Human DnaJ (Hsp40) homolog, subfamily B, member 8 (DNAJB8) 10 ug
$600.00
SC306587 DNAJB8 (untagged)-Human DnaJ (Hsp40) homolog, subfamily B, member 8 (DNAJB8) 10 ug
$330.00

Plasmid Map for RG207352

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for RG207352
Circular map for RG207352

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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