DAP5 (EIF4G2) (NM_001418) Human Tagged ORF Clone

SKU RG207227

EIF4G2 (tGFP-tagged) - Human eukaryotic translation initiation factor 4 gamma, 2 (EIF4G2), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: DAP5
• Synonyms: AAG1; DAP5; NAT1; P97
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207227 representing NM_001418; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; GTGGAGAGTGCGATTGCAGAAGGGGGTGCTTCTCGTTTCAGTGCTTCTTCGGGCGGAGGAGGAAGTAGGG; GTGCACCTCAGCACTATCCCAAGACTGCTGGCAACAGCGAGTTCCTGGGGAAAACCCCAGGGCAAAACGC; TCAGAAATGGATTCCTGCACGAAGCACTAGACGAGATGACAACTCCGCAGCAAACAACTCCGCAAACGAA; AAAGAACGACATGATGCAATCTTCAGGAAAGTAAGAGGCATACTAAATAAGCTTACTCCTGAAAAGTTTG; ACAAGCTATGCCTTGAGCTCCTCAATGTGGGTGTAGAGTCTAAACTCATCCTTAAAGGGGTCATACTGCT; GATTGTGGACAAAGCCCTAGAAGAGCCAAAGTATAGCTCACTGTATGCTCAGCTATGTCTGCGATTGGCA; GAAGATGCACCAAACTTTGATGGCCCAGCAGCAGAGGGTCAACCAGGACAGAAGCAAAGCACCACATTCA; GACGCCTCCTAATTTCCAAATTACAAGATGAATTTGAAAACCGAACTAGAAATGTTGATGTCTATGATAA; GCGTGAAAATCCCCTCCTCCCCGAGGAGGAGGAACAGAGAGCCATTGCTAAGATCAAGATGTTGGGAAAC; ATCAAATTCATTGGAGAGCTTGGCAAGCTTGATCTTATTCACGAATCTATCCTTCATAAGTGCATCAAAA; CACTTTTGGAAAAGAAGAAGAGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCTGTCAGAT; AATGAGGACAGTGGGACCTAGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCGA; ATGTGCTCCTTGATGTTAAGTAAGGAATTGCCAGCAAGGATTCGTTTCCTGCTGCAGGATACCGTAGAGT; TGCGAGAACACCATTGGGTTCCTCGCAAGGCTTTTCTTGACAATGGACCAAAGACGATCAATCAAATTCG; TCAAGATGCAGTAAAAGATCTAGGGGTGTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGACTTC; TTTCTGGAGGGACCGTTCATGCCACCCAGGATGAAAATGGATAGGGACCCACTTGGAGGACTTGCTGATA; TGTTTGGACAAATGCCAGGTAGCGGAATTGGTACTGGTCCAGGAGTTATCCAGGATAGATTTTCACCCAC; CATGGGACGTCATCGTTCAAATCAACTCTTCAATGGCCATGGGGGACACATCATGCCTCCCACACAATCG; CAGTTTGGAGAGATGGGAGGCAAGTTTATGAAAAGCCAGGGGCTAAGCCAGCTCTACCATAACCAGAGTC; AGGGACTCTTATCCCAGCTGCAAGGACAGTCGAAGGATATGCCACCTCGGTTTTCTAAGAAAGGACAGCT; TAATGCAGATGAGATTAGCCTGAGGCCTGCTCAGTCGTTCCTAATGAATAAAAATCAAGTGCCAAAGCTT; CAGCCCCAGATAACTATGATTCCTCCTAGTGCACAACCACCACGCACTCAAACACCACCTCTGGGACAGA; CACCTCAGCTTGGTCTCAAAACTAATCCACCACTTATCCAGGAAAAGCCTGCCAAGACCAACAAAAAGCC; ACCACCGTCAAAGGAAGAACTCCTTAAACTAACTGAAACTGTTGTGACTGAATATCTAAATAGTGGAAAT; GCAAATGAGGCTGTCAATGGTGTAAGAGAAATGAGGGCTCCTAAACACTTTCTTCCTGAGATGTTAAGCA; AAGTAATCATCCTGTCACTAGATAGAAGCGATGAAGATAAAGAAAAAGCAAGTTCTTTGATCAGTTTACT; CAAACAGGAAGGGATAGCCACAAGTGACAACTTCATGCAGGCTTTCCTGAATGTATTGGACCAGTGTCCC; AAACTGGAGGTTGACATCCCTTTGGTGAAATCCTATTTAGCACAGTTTGCAGCTCGTGCCATCATTTCAG; AGCTGGTGAGCATTTCAGAACTAGCTCAACCACTAGAAAGTGGCACCCATTTTCCTCTCTTCCTACTTTG; TCTTCAGCAGTTAGCTAAATTACAAGATCGAGAATGGTTAACAGAACTTTTTCAACAAAGCAAGGTCAAT; ATGCAGAAAATGCTCCCAGAAATTGATCAGAATAAGGACCGCATGTTGGAGATTTTGGAAGGAAAGGGAC; TGAGTTTCTTATTCCCACTCCTCAAATTGGAGAAGGAACTGTTGAAGCAAATAAAGTTGGATCCATCCCC; TCAAACCATATATAAATGGATTAAAGATAACATCTCTCCCAAACTTCATGTAGATAAAGGATTTGTGAAC; ATCTTAATGACTAGCTTCTTACAGTACATTTCTAGTGAAGTAAACCCCCCCAGCGATGAAACAGATTCAT; CCTCTGCTCCTTCCAAAGAACAGTTAGAGCAGGAAAAACAACTACTACTATCTTTCAAGCCAGTAATGCA; GAAATTTCTTCATGATCACGTTGATCTACAAGTCAGTGCCCTGTATGCTCTCCAGGTGCACTGCTATAAC; AGCAACTTCCCAAAAGGCATGTTACTTCGCTTTTTTGTGCACTTCTATGACATGGAAATTATTGAAGAAG; AAGCTTTCTTGGCTTGGAAAGAAGATATAACCCAAGAGTTTCCGGGAAAAGGCAAGGCTTTGTTCCAGGT; GAATCAGTGGCTAACCTGGTTAGAAACTGCTGAAGAAGAAGAATCAGAGGAAGAAGCTGAC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207227 representing NM_001418; Red=Cloning site Green=Tags(s); ; VESAIAEGGASRFSASSGGGGSRGAPQHYPKTAGNSEFLGKTPGQNAQKWIPARSTRRDDNSAANNSANE; KERHDAIFRKVRGILNKLTPEKFDKLCLELLNVGVESKLILKGVILLIVDKALEEPKYSSLYAQLCLRLA; EDAPNFDGPAAEGQPGQKQSTTFRRLLISKLQDEFENRTRNVDVYDKRENPLLPEEEEQRAIAKIKMLGN; IKFIGELGKLDLIHESILHKCIKTLLEKKKRVQLKDMGEDLECLCQIMRTVGPRLDHERAKSLMDQYFAR; MCSLMLSKELPARIRFLLQDTVELREHHWVPRKAFLDNGPKTINQIRQDAVKDLGVFIPAPMAQGMRSDF; FLEGPFMPPRMKMDRDPLGGLADMFGQMPGSGIGTGPGVIQDRFSPTMGRHRSNQLFNGHGGHIMPPTQS; QFGEMGGKFMKSQGLSQLYHNQSQGLLSQLQGQSKDMPPRFSKKGQLNADEISLRPAQSFLMNKNQVPKL; QPQITMIPPSAQPPRTQTPPLGQTPQLGLKTNPPLIQEKPAKTNKKPPPSKEELLKLTETVVTEYLNSGN; ANEAVNGVREMRAPKHFLPEMLSKVIILSLDRSDEDKEKASSLISLLKQEGIATSDNFMQAFLNVLDQCP; KLEVDIPLVKSYLAQFAARAIISELVSISELAQPLESGTHFPLFLLCLQQLAKLQDREWLTELFQQSKVN; MQKMLPEIDQNKDRMLEILEGKGLSFLFPLLKLEKELLKQIKLDPSPQTIYKWIKDNISPKLHVDKGFVN; ILMTSFLQYISSEVNPPSDETDSSSAPSKEQLEQEKQLLLSFKPVMQKFLHDHVDLQVSALYALQVHCYN; SNFPKGMLLRFFVHFYDMEIIEEEAFLAWKEDITQEFPGKGKALFQVNQWLTWLETAEEEESEEEAD; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001418
• ORF Size: 2721 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001418.3, NP_001409.3
• RefSeq Size: 3911 bp
• RefSeq ORF: 2724 bp
• Locus ID: 1982
• UniProt ID: P78344
• Cytogenetics: 11p15.4
• Domains: eIF5C, MA3
• Protein Families: Transcription Factors
• Protein Pathways: Viral myocarditis
• Summary: Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]