PDE2A (NM_002599) Human Tagged ORF Clone
- TrueORF®
SKU
RG207219
PDE2A (tGFP-tagged) - Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 1
$789.00
$1,062.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PDE2A |
| Synonyms | CGS-PDE; cGSPDE; PDE2A1; PED2A4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_002599 |
| ORF Size | 2823 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002599.1, NP_002590.1 |
| RefSeq Size | 4240 bp |
| RefSeq ORF | 2826 bp |
| Locus ID | 5138 |
| UniProt ID | O00408 |
| Cytogenetics | 11q13.4 |
| Domains | GAF, HDc, PDEase |
| Protein Families | Druggable Genome |
| Protein Pathways | Progesterone-mediated oocyte maturation, Purine metabolism |
| Summary | Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Plays an important role in growth and invasion of malignant melanoma cells (e.g. pseudomyxoma peritonei (PMP) cell line) (PubMed:24705027).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002599" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC207219 | PDE2A (Myc-DDK-tagged)-Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 1 | 10 ug |
$862.00
|
|
| RC207219L1 | Lenti ORF clone of Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,162.00
|
|
| RC207219L2 | Lenti ORF clone of Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 1, mGFP tagged | 10 ug |
$1,162.00
|
|
| RC207219L3 | Lenti ORF clone of Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,162.00
|
|
| RC207219L4 | Lenti ORF clone of Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 1, mGFP tagged | 10 ug |
$1,162.00
|
|
| SC110970 | PDE2A (untagged)-Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 1 | 10 ug |
$862.00
|
|
Citations
Phosphodiesterase 2A and 3B variants are associated with primary aldosteronism
Rassi-Cruz, Maria, Faucz et al
Endocr Relat Cancer (2020)
Rassi-Cruz, Maria, Faucz et al
Endocr Relat Cancer (2020)
AI Summary: OriGene products contributed to this study by providing the pCMV6-AC-GFP vector (RG207219) and the human Myc-DDK-tagged expression plasmid (PS100001), which were used for cloning and expressing wild-type and mutant PDE2A in functional assays to assess the impact of identified genetic variants.
PDE2A2 regulates mitochondria morphology and apoptotic cell death via local modulation of cAMP/PKA signalling
Monterisi, Lobo, Livie et al
Elife (2017) 6 DOI: 10.7554/eLife.21374
Monterisi, Lobo, Livie et al
Elife (2017) 6 DOI: 10.7554/eLife.21374
AI Summary: OriGene products PDE2A1 (RG235036), PDE2A2 (RG226806), and PDE2A3 (RG207219) in pCMV6-AC-GFP were used to express and study different PDE2A isoforms, enabling the authors to investigate their roles in mitochondrial cAMP/PKA signalling and regulation of mitochondrial morphology and apoptosis.
Snippet: The EGFP-tagged version of PDE2A2 was generated by subcloning PDE2A2 as NheI fragment at the N-terminus of EGFP (pEYFP-C1-EGFP). PDE2A1 (RG235036), PDE2A2 (RG226806) and PDE2A3 (RG207219) in pCMV6-AC-GFP were from Origene (MD, USA)
Showing 1-2 of 2 papers.
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