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Probable hydrolase PNKD (PNKD) (NM_015488) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG206179
PNKD (tGFP-tagged) - Human paroxysmal nonkinesigenic dyskinesia (PNKD), nuclear gene encoding mitochondrial protein, transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $657.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Probable hydrolase PNKD
Synonyms BRP17; DYT8; FKSG19; FPD1; KIPP1184; MR-1; MR-1S; MR1; PDC; PKND1; PNKD1; R1; TAHCCP2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG206179 representing NM_015488
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGTGGTAGCTGCTACGGCGCTGAAGAGCCGGGGGGCGAGAAATGCCCGCGTCCTCCGGGGGA
TTCTCGCAGGAGCCACAGCTAACAAGGTTTCTCATAACAGGACCCGGGCCCTGCAAAGCCACAGCTCCTC
AGAGGGCAAGGAGGAACCTGAACCCCTATCCCCGGAGCTGGAATACATTCCCAGAAAGAGGGGCAAGAAC
CCCATGAAAGCTGTGGGACTGGCCTGGTACAGCCTGTACACCCGCACCTGGCTCGGGTACCTCTTCTACC
GACAGCAGCTGCGCAGGGCTCGGAATCGCTACCCTAAAGGCCACTCGAAAACCCAGCCCCGCCTCTTCAA
TGGAGTGAAGGTGCTTCCCATCCCTGTCCTCTCGGACAACTACAGCTACCTCATCATCGACACCCAGGCC
CAGCTGGCTGTGGCTGTGGACCCTTCAGACCCTCGGGCTGTGCAGGCTTCCATTGAAAAGGAAGGGGTCA
CCTTGGTCGCCATTCTGTGTACTCACAAGCACTGGGACCACAGTGGAGGGAACCGTGACCTCAGCCGGCG
GCACCGGGACTGTCGGGTGTACGGGAGCCCTCAGGACGGCATCCCCTACCTCACCCATCCCCTGTGTCAT
CAAGATGTGGTCAGCGTGGGACGGCTTCAGATCCGGGCCCTGGCTACACCTGGCCACACACAAGGCCATC
TGGTCTACCTACTGGATGGGGAGCCCTACAAGGGTCCCTCCTGCCTCTTCTCAGGGGACCTGCTCTTCCT
CTCTGGCTGTGGGCGGACCTTTGAGGGCAATGCAGAGACCATGCTGAGCTCACTGGACACTGTGCTGGGG
CTAGGGGATGACACCCTTCTGTGGCCTGGTCATGAGTATGCAGAGGAGAACCTGGGCTTTGCAGGTGTGG
TGGAGCCCGAGAACCTGGCCCGGGAGAGGAAGATGCAGTGGGTGCAGCGGCAGCGGCTGGAGCGCAAGGG
CACGTGCCCATCTACCCTGGGAGAGGAGCGCTCCTACAACCCGTTCCTGAGAACCCACTGCCTGGCGCTA
CAGGAGGCTCTGGGGCCGGGGCCGGGCCCCACTGGGGATGATGACTACTCCCGGGCCCAGCTCCTGGAAG
AGCTCCGCCGGCTGAAGGATATGCACAAGAGCAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG206179 representing NM_015488
Red=Cloning site Green=Tags(s)

MAAVVAATALKSRGARNARVLRGILAGATANKVSHNRTRALQSHSSSEGKEEPEPLSPELEYIPRKRGKN
PMKAVGLAWYSLYTRTWLGYLFYRQQLRRARNRYPKGHSKTQPRLFNGVKVLPIPVLSDNYSYLIIDTQA
QLAVAVDPSDPRAVQASIEKEGVTLVAILCTHKHWDHSGGNRDLSRRHRDCRVYGSPQDGIPYLTHPLCH
QDVVSVGRLQIRALATPGHTQGHLVYLLDGEPYKGPSCLFSGDLLFLSGCGRTFEGNAETMLSSLDTVLG
LGDDTLLWPGHEYAEENLGFAGVVEPENLARERKMQWVQRQRLERKGTCPSTLGEERSYNPFLRTHCLAL
QEALGPGPGPTGDDDYSRAQLLEELRRLKDMHKSK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015488
ORF Size 1155 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015488.3
RefSeq Size 3129 bp
RefSeq ORF 1158 bp
Locus ID 25953
UniProt ID Q8N490
Cytogenetics 2q35
Protein Families Transmembrane
Summary This gene is thought to play a role in the regulation of myofibrillogenesis. Mutations in this gene have been associated with the movement disorder paroxysmal non-kinesigenic dyskinesia. Alternative splicing results in multiple transcript variants. provided by RefSeq, Mar 2010
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"NM_015488" in other vectors (5)
SKU Description Size Price
RC206179 PNKD (Myc-DDK-tagged)-Human paroxysmal nonkinesigenic dyskinesia (PNKD), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$289.00 $457.00
RC206179L3 Lenti ORF clone of Human paroxysmal nonkinesigenic dyskinesia (PNKD), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC206179L4 Lenti ORF clone of Human paroxysmal nonkinesigenic dyskinesia (PNKD), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged 10 ug
$757.00
SC100172 PNKD (untagged)-Human paroxysmal nonkinesigenic dyskinesia (PNKD), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$908.00
SC317618 PNKD (untagged)-Human paroxysmal nonkinesigenic dyskinesia (PNKD), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$503.00

Plasmid Map for RG206179

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG206179
Circular map for RG206179

file-alt Citations

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