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C18orf54 (NM_173529) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG206158
C18orf54 (tGFP-tagged) - Human chromosome 18 open reading frame 54 (C18orf54)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol C18orf54
Synonyms LAS2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG206158 representing NM_173529
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGAAATCAAAGACAAAACATAGACTTTGTTCTCAGGAATCTTCAGTATCTGCCCTGCTGGCAAGCT
GCACCCTGAGTGGTAGTAATTCCTCTAATTCTGATGGCTCGTTTCACTATAAAGATAAGCTGTACAGATC
TGCTTCTCAAGCTCTACAGGCTTATATTGATGATTTTGATCTAGGCCAAATATATCCTGGTGCAAGCACT
GGAAAAATTAACATTGATGAGGATTTTACTAATATGTCACAGTTCTGCAACTATATTTACAAACCAAACA
ATGCTTTTGAAAACCTTGATCACAAAAAGCACTCAAACTTCATATCCTGTAGAAGACACACCGTTAATGA
CATAGACTCCATGAGCCTAACAACTGATGATCTATTAAGACTCCCAGCAGATGGATCATTTTCTTATACT
TATGTTGGACCGAGTCACCGAACGAGCAAGAAAAACAAGAAATGCCGTGGAAGACTGGGTTCATTGGACA
TTGAGAAGAATCCACATTTTCAAGGACCCTACACTTCCATGGGCAAGGATAACTTTGTTACTCCTGTTAT
ACGCTCAAATATAAATGGAAAGCAATGTGGTGACAAAATTGAATTGCTTATCTTGAAGGCCAAGAGAAAT
CTAGAGCAGTGTACTGAAGAATTACCAAAGTCCATGAAAAAGGATGACAGTCCTTGCTCATTAGATAAAC
TTGAAGCAGACAGATCATGGGAAAATATTCCTGTTACTTTCAAATCTCCTGTTCCCGTTAACTCTGATGA
TAGTCCTCAACAAACTTCAAGGGCAAAGAGTGCTAAAGGGGTTCTTGAAGACTTTCTAAATAATGATAAT
CAGAGCTGTACTCTCTCTGGAGGCAAACATCATGGTCCTGTTGAAGCCCTGAAACAAATGTTATTTAACC
TTCAAGCAGTACAAGAACGTTTTAATCAAAATAAGACCACAGATCCAAAAGAAGAGATTAAACAAGTTTC
AGAAGATGATTTCTCTAAATTACAGTTGAAGGAAAGTATGATTCCTATTACTAGGTCACTTCAGAAGGCT
TTGCACCATTTATCTCGCCTGAGAGACCTGGTTGATGATACGAATGGAGAACGGTCACCGAAAATG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG206158 representing NM_173529
Red=Cloning site Green=Tags(s)

MAKSKTKHRLCSQESSVSALLASCTLSGSNSSNSDGSFHYKDKLYRSASQALQAYIDDFDLGQIYPGAST
GKINIDEDFTNMSQFCNYIYKPNNAFENLDHKKHSNFISCRRHTVNDIDSMSLTTDDLLRLPADGSFSYT
YVGPSHRTSKKNKKCRGRLGSLDIEKNPHFQGPYTSMGKDNFVTPVIRSNINGKQCGDKIELLILKAKRN
LEQCTEELPKSMKKDDSPCSLDKLEADRSWENIPVTFKSPVPVNSDDSPQQTSRAKSAKGVLEDFLNNDN
QSCTLSGGKHHGPVEALKQMLFNLQAVQERFNQNKTTDPKEEIKQVSEDDFSKLQLKESMIPITRSLQKA
LHHLSRLRDLVDDTNGERSPKM

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173529
ORF Size 1116 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173529.6
RefSeq Size 5016 bp
RefSeq ORF 1119 bp
Locus ID 162681
UniProt ID Q8IYD9
Cytogenetics 18q21.2
Protein Families Secreted Protein
Summary Might play a role in cell proliferation.UniProtKB/Swiss-Prot Function
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"NM_173529" in other vectors (4)
SKU Description Size Price
RC206158 C18orf54 (Myc-DDK-tagged)-Human chromosome 18 open reading frame 54 (C18orf54) 10 ug
$457.00
RC206158L3 Lenti ORF clone of Human chromosome 18 open reading frame 54 (C18orf54), Myc-DDK-tagged 10 ug
$757.00
RC206158L4 Lenti ORF clone of Human chromosome 18 open reading frame 54 (C18orf54), mGFP tagged 10 ug
$757.00
SC310472 C18orf54 (untagged)-Human chromosome 18 open reading frame 54 (C18orf54) 10 ug
$503.00

Plasmid Map for RG206158

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG206158
Circular map for RG206158

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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