FTSJD1 (CMTR2) (NM_018348) Human Tagged ORF Clone

SKU RG206109

FTSJD1 (tGFP-tagged) - Human FtsJ methyltransferase domain containing 1 (FTSJD1), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: FTSJD1
• Synonyms: AFT; FTSJD1; HMTr2; MTr2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206109 representing NM_018348; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGTAAGTGCAGAAAGACACCAGTTCAGCAGCTAGCAAGTCCCGCGTCATTCAGCCCAGATATTCTTG; CTGACATTTTTGAACTCTTTGCCAAGAACTTTTCTTATGGCAAGCCACTTAATAATGAGTGGCAGTTACC; AGATCCCAGTGAGATTTTCACCTGTGACCACACTGAACTTAATGCATTTCTTGATTTGAAGAACTCCCTA; AATGAAGTAAAAAACCTACTGAGTGATAAGAAACTGGATGAGTGGCATGAGCACACTGCTTTCACTAATA; AAGCGGGGAAAATCATTTCTCATGTTAGAAAATCTGTGAATGCTGAACTTTGTACTCAAGCATGGTGTAA; GTTCCATGAGATTTTGTGCAGCTTTCCACTTATTCCACAGGAAGCTTTTCAGAATGGAAAACTGAATTCT; CTACACCTTTGTGAAGCTCCAGGAGCTTTTATAGCTAGTCTCAACCACTACTTAAAATCCCATCGGTTTC; CTTGTCATTGGAGTTGGGTAGCGAATACTCTGAATCCATACCATGAAGCAAATGACGACCTCATGATGAT; TATGGATGACCGGCTTATTGCAAATACCTTGCACTGGTGGTACTTTGGTCCAGATAACACTGGTGATATC; ATGACCCTGAAATTCTTGACTGGACTTCAGAATTTCATAAGCAGCATGGCTACTGTTCACTTGGTCACTG; CAGATGGGAGTTTTGATTGCCAAGGAAACCCAGGTGAACAAGAAGCTTTAGTTTCTTCTTTGCATTACTG; TGAAGTTGTCACTGCTCTGACCACTCTTGGAAACGGTGGCTCTTTTGTTCTAAAGATGTTTACTATGTTT; GAACATTGTTCCATAAACTTGATGTACCTGCTAAACTGTTGTTTTGACCAAGTCCATGTTTTCAAACCTG; CTACTAGCAAGGCAGGAAACTCCGAAGTCTATGTGGTTTGCCTCCACTATAAGGGGAGAGAGGCCATCCA; TCCTCTGTTATCTAAGATGACCTTGAATTTTGGGACTGAAATGAAAAGGAAAGCCCTTTTTCCCCATCAT; GTGATTCCTGATTCTTTTCTTAAGAGACATGAAGAATGTTGTGTGTTCTTTCATAAATATCAGCTAGAGA; CTATTTCTGAAAACATTCGTCTATTTGAGTGCATGGGAAAGGCGGAACAAGAAAAGCTGAATAATTTAAG; GGATTGTGCTATACAATATTTTATGCAAAAATTTCAACTGAAACATCTTTCCAGAAATAATTGGCTAGTA; AAAAAATCTAGTATTGGTTGTAGTACAAATACAAAATGGTTTGGGCAGAGGAACAAATATTTTAAAACTT; ATAATGAAAGGAAGATGCTAGAAGCCCTTTCATGGAAAGATAAAGTAGCCAAAGGATACTTTAATAGTTG; GGCTGAAGAACATGGTGTATATCATCCTGGGCAGAGTTCTATTTTAGAAGGAACAGCTTCCAATCTTGAG; TGTCACTTATGGCATATTTTGGAGGGAAAGAAACTGCCAAAGGTAAAATGTTCTCCTTTTTGCAATGGTG; AAATTTTAAAAACTCTTAATGAAGCAATTGAAAAGTCATTAGGAGGAGCTTTTAATTTGGATTCCAAGTT; TAGGCCAAAACAGCAGTATTCTTGTTCTTGTCATGTTTTTTCTGAAGAACTGATATTTTCCGAGTTGTGT; AGCCTTACTGAGTGCCTTCAGGATGAGCAGGTTGTAGTACCCAGCAATCAAATAAAGTGCCTGCTGGTGG; GCTTTTCGACTCTCCGTAATATCAAAATGCATATACCGTTGGAAGTTCGACTCCTAGAATCAGCTGAACT; CACAACTTTTAGCTGTTCATTGCTTCATGATGGAGATCCAACTTACCAGCGTTTATTTTTGGACTGCCTT; CTACATTCATTGCGGGAGCTTCATACAGGAGATGTTATGATTTTGCCTGTACTTTCTTGCTTCACAAGAT; TTATGGCTGGTTTGATCTTTGTACTCCACAGTTGTTTTAGATTCATCACTTTTGTTTGTCCCACATCCTC; TGATCCCCTGAGGACCTGCGCAGTCCTGCTATGTGTTGGTTATCAGGACCTTCCAAATCCAGTTTTCCGA; TATTTGCAGAGTGTGAATGAATTGTTGAGCACTTTGCTCAACTCTGACTCACCCCAGCAGGTTTTACAGT; TTGTGCCAATGGAGGTACTCCTTAAGGGGGCCCTGCTTGATTTTTTGTGGGATTTGAATGCTGCCATTGC; TAAAAGGCATTTGCATTTCATTATTCAAAGAGAGAGAGAAGAAATTATCAACAGCCTTCAGTTACAAAAC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206109 representing NM_018348; Red=Cloning site Green=Tags(s); ; MSKCRKTPVQQLASPASFSPDILADIFELFAKNFSYGKPLNNEWQLPDPSEIFTCDHTELNAFLDLKNSL; NEVKNLLSDKKLDEWHEHTAFTNKAGKIISHVRKSVNAELCTQAWCKFHEILCSFPLIPQEAFQNGKLNS; LHLCEAPGAFIASLNHYLKSHRFPCHWSWVANTLNPYHEANDDLMMIMDDRLIANTLHWWYFGPDNTGDI; MTLKFLTGLQNFISSMATVHLVTADGSFDCQGNPGEQEALVSSLHYCEVVTALTTLGNGGSFVLKMFTMF; EHCSINLMYLLNCCFDQVHVFKPATSKAGNSEVYVVCLHYKGREAIHPLLSKMTLNFGTEMKRKALFPHH; VIPDSFLKRHEECCVFFHKYQLETISENIRLFECMGKAEQEKLNNLRDCAIQYFMQKFQLKHLSRNNWLV; KKSSIGCSTNTKWFGQRNKYFKTYNERKMLEALSWKDKVAKGYFNSWAEEHGVYHPGQSSILEGTASNLE; CHLWHILEGKKLPKVKCSPFCNGEILKTLNEAIEKSLGGAFNLDSKFRPKQQYSCSCHVFSEELIFSELC; SLTECLQDEQVVVPSNQIKCLLVGFSTLRNIKMHIPLEVRLLESAELTTFSCSLLHDGDPTYQRLFLDCL; LHSLRELHTGDVMILPVLSCFTRFMAGLIFVLHSCFRFITFVCPTSSDPLRTCAVLLCVGYQDLPNPVFR; YLQSVNELLSTLLNSDSPQQVLQFVPMEVLLKGALLDFLWDLNAAIAKRHLHFIIQREREEIINSLQLQN; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018348
• ORF Size: 2310 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018348.6
• RefSeq Size: 4184 bp
• RefSeq ORF: 2313 bp
• Locus ID: 55783
• UniProt ID: Q8IYT2
• Cytogenetics: 16q22.2
• Domains: FtsJ
• Summary: S-adenosyl-L-methionine-dependent methyltransferase that mediates mRNA cap2 2'-O-ribose methylation to the 5'-cap structure of mRNAs. Methylates the ribose of the second nucleotide of a m(7)GpppG-capped mRNA and small nuclear RNA (snRNA) (cap0) to produce m(7)GpppRmpNm (cap2). Recognizes a guanosine cap on RNA independently of its N(7) methylation status. Display cap2 methylation on both cap0 and cap1. Displays a preference for cap1 RNAs.[UniProtKB/Swiss-Prot Function]