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FTSJD1 (CMTR2) (NM_018348) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC206109L4
Lenti-ORF clone of FTSJD1 (mGFP-tagged)-Human FtsJ methyltransferase domain containing 1 (FTSJD1), transcript variant 1
ORF Plasmid:
mGFP DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $1,076.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FTSJD1
Synonyms AFT; FTSJD1; HMTr2; MTr2
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC206109).
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_018348
ORF Size 2310 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018348.5
RefSeq Size 4184 bp
RefSeq ORF 2313 bp
Locus ID 55783
UniProt ID Q8IYT2
Cytogenetics 16q22.2
Domains FtsJ
MW 88.1 kDa
Summary S-adenosyl-L-methionine-dependent methyltransferase that mediates mRNA cap2 2'-O-ribose methylation to the 5'-cap structure of mRNAs. Methylates the ribose of the second nucleotide of a m(7)GpppG-capped mRNA and small nuclear RNA (snRNA) (cap0) to produce m(7)GpppRmpNm (cap2). Recognizes a guanosine cap on RNA independently of its N(7) methylation status. Display cap2 methylation on both cap0 and cap1. Displays a preference for cap1 RNAs.UniProtKB/Swiss-Prot Function
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"NM_018348" in other vectors (4)
SKU Description Size Price
RC206109 FTSJD1 (Myc-DDK-tagged)-Human FtsJ methyltransferase domain containing 1 (FTSJD1), transcript variant 1 10 ug
$776.00
RC206109L3 Lenti-ORF clone of FTSJD1 (Myc-DDK-tagged)-Human FtsJ methyltransferase domain containing 1 (FTSJD1), transcript variant 1 10 ug
$1,076.00
RG206109 FTSJD1 (tGFP-tagged) - Human FtsJ methyltransferase domain containing 1 (FTSJD1), transcript variant 1 10 ug
$976.00
SC113563 FTSJD1 (untagged)-Human FtsJ methyltransferase domain containing 1 (FTSJD1), transcript variant 1 10 ug
$706.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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