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FBXL5 (NM_033535) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG206089
FBXL5 (tGFP-tagged) - Human F-box and leucine-rich repeat protein 5 (FBXL5), transcript variant 2
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $834.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FBXL5
Synonyms F-box and leucine-rich repeat protein 5; F-box protein FBL5; FBL4; FBL5; FLR1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG206089 representing NM_033535
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCCCTTTCCTGAAGAAGTGGACGTCTTCACCGCCCCACACTGGCGGATGAAGCAGCTGGTGGGGC
TCTACTGCGACAAGCTTTCTAAAACCAATTTTTCCAACAACAACGATTTCCGTGCTCTTCTGCAGTCTTT
GTATGCTACTTTCACGGAGTTCAAAATGCATGAGCAGATTGAAAATGAATACATTATTGGTTTGCTTCAA
CAACGCAGCCAGACCATTTATAATGTACATTCTGACAATAAACTCTCCGAGATGCTTAGCCTCTTTGAAA
AGGGACTGAAGAATGTTAAGAATGAATATGAACAGTTAAATTATGCAAAACAACTGAAAGAGAGATTGGA
GGCTTTTACAAGAGATTTTCTTCCTCACATGAAAGAGGAAGAGGAGGTTTTTCAGCCCATGTTAATGGAA
TATTTTACCTATGAAGAGCTTAAGGATATTAAAAAGAAAGTGATTGCACAACACTGCTCTCAGAAGGATA
CTGCAGAACTCCTTAGAGGTCTTAGCCTATGGAATCATGCTGAAGAGCGACAGAAGTTTTTTAAATATTC
CGTGGATGAAAAGTCAGATAAAGAAGCAGAAGTGTCAGAACACTCCACAGGTATAACCCATCTTCCTCCT
GAGGTAATGCTGTCAATTTTCAGCTATCTTAATCCTCAAGAGTTATGTCGATGCAGTCAAGTAAGCATGA
AATGGTCTCAGCTGACAAAAACGAGATCGCTTTGGAAACATCTTTACCCTGTTCATTGGGCCAGAGGTGA
CTGGTATAGTGGTCCCGCAACTGAACTTGATACTGAACCTGATGATGAATGGGTGAAAAATAGGAAAGAT
GAAAGTCGTGCTTTTCATGAGTGGGATGAAGATGCTGACATTGATGAATCTGAAGAGTCTGCGGAGGAAT
CAATTGCTATCAGCATTGCACAAATGGAAAAACGTTTACTCCATGGCTTAATTCATAACGTTCTACCATA
TGTTGGTACTTCTGTAAAAACCTTAGTATTAGCATACAGCTCTGCAGTTTCCAGCAAAATGGTTAGGCAG
ATTTTAGAGCTTTGTCCTAACCTGGAGCATCTGGATCTTACCCAGACTGACATTTCAGATTCTGCATTTG
ACAGTTGGTCTTGGCTTGGTTGCTGCCAGAGTCTTCGGCATCTTGATCTGTCTGGTTGTGAGAAAATCAC
AGATGTGGCCCTAGAGAAGATTTCCAGAGCTCTTGGAATTCTGACATCTCATCAAAGTGGCTTTTTGAAA
ACATCTACAAGCAAAATTACTTCAACTGCGTGGAAAAATAAAGACATTACCATGCAGTCCACCAAGCAGT
ATGCCTGTTTGCACGATTTAACTAACAAGGGCATTGGAGAAGAAATAGATAATGAACACCCCTGGACTAA
GCCTGTTTCTTCTGAGAATTTCACTTCTCCTTATGTGTGGATGTTAGATGCTGAAGATTTGGCTGATATT
GAAGATACTGTGGAATGGAGACATAGAAATGTTGAAAGTCTTTGTGTAATGGAAACAGCATCCAACTTTA
GTTGTTCCACCTCTGGTTGTTTTAGTAAGGACATTGTTGGACTAAGGACTAGTGTCTGTTGGCAGCAGCA
TTGTGCTTCTCCAGCCTTTGCGTATTGTGGTCACTCATTTTGTTGTACAGGAACAGCTTTAAGAACTATG
TCATCACTCCCAGAATCTTCTGCAATGTGTAGAAAAGCAGCAAGGACTAGATTGCCTAGGGGAAAAGACT
TAATTTACTTTGGGAGTGAAAAATCTGATCAAGAGACTGGACGTGTACTTCTGTTTCTCAGTTTATCTGG
ATGTTATCAGATCACAGACCATGGTCTCAGGGTTTTGACTCTGGGAGGAGGGCTGCCTTATTTGGAGCAC
CTTAATCTCTCTGGTTGTCTTACTATAACTGGTGCAGGCCTGCAGGATTTGGTTTCAGCATGTCCTTCTC
TGAATGATGAATACTTTTACTACTGTGACAACATTAACGGTCCTCATGCTGATACCGCCAGTGGATGCCA
GAATTTGCAGTGTGGTTTTCGAGCCTGCTGCCGCTCTGGCGAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG206089 representing NM_033535
Red=Cloning site Green=Tags(s)

MAPFPEEVDVFTAPHWRMKQLVGLYCDKLSKTNFSNNNDFRALLQSLYATFTEFKMHEQIENEYIIGLLQ
QRSQTIYNVHSDNKLSEMLSLFEKGLKNVKNEYEQLNYAKQLKERLEAFTRDFLPHMKEEEEVFQPMLME
YFTYEELKDIKKKVIAQHCSQKDTAELLRGLSLWNHAEERQKFFKYSVDEKSDKEAEVSEHSTGITHLPP
EVMLSIFSYLNPQELCRCSQVSMKWSQLTKTRSLWKHLYPVHWARGDWYSGPATELDTEPDDEWVKNRKD
ESRAFHEWDEDADIDESEESAEESIAISIAQMEKRLLHGLIHNVLPYVGTSVKTLVLAYSSAVSSKMVRQ
ILELCPNLEHLDLTQTDISDSAFDSWSWLGCCQSLRHLDLSGCEKITDVALEKISRALGILTSHQSGFLK
TSTSKITSTAWKNKDITMQSTKQYACLHDLTNKGIGEEIDNEHPWTKPVSSENFTSPYVWMLDAEDLADI
EDTVEWRHRNVESLCVMETASNFSCSTSGCFSKDIVGLRTSVCWQQHCASPAFAYCGHSFCCTGTALRTM
SSLPESSAMCRKAARTRLPRGKDLIYFGSEKSDQETGRVLLFLSLSGCYQITDHGLRVLTLGGGLPYLEH
LNLSGCLTITGAGLQDLVSACPSLNDEYFYYCDNINGPHADTASGCQNLQCGFRACCRSGE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_033535
ORF Size 2073 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_033535.2, NP_277077.1
RefSeq Size 3475 bp
RefSeq ORF 1697 bp
Locus ID 26234
Cytogenetics 4p15.32
Domains F-box, LRR, LRR_CC
Protein Families Druggable Genome
Summary This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains several tandem leucine-rich repeats. Alternatively spliced transcript variants have been described for this locus. provided by RefSeq, Aug 2010
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"NM_033535" in other vectors (4)
SKU Description Size Price
RC206089 FBXL5 (Myc-DDK-tagged)-Human F-box and leucine-rich repeat protein 5 (FBXL5), transcript variant 2 10 ug
$634.00
RC206089L3 Lenti ORF clone of Human F-box and leucine-rich repeat protein 5 (FBXL5), transcript variant 2, Myc-DDK-tagged 10 ug
$934.00
RC206089L4 Lenti ORF clone of Human F-box and leucine-rich repeat protein 5 (FBXL5), transcript variant 2, mGFP tagged 10 ug
$934.00
SC110365 FBXL5 (untagged)-Human F-box and leucine-rich repeat protein 5 (FBXL5), transcript variant 2 10 ug
$527.00

Plasmid Map for RG206089

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG206089
Circular map for RG206089

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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