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NSF (NM_006178) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG205983
NSF (tGFP-tagged) - Human N-ethylmaleimide-sensitive factor (NSF)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
$789.00 $881.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NSF
Synonyms SEC18; SKD2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG205983 representing NM_006178
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGCCGGAGCATGCAAGCGGCAAGATGTCCTACAGATGAATTATCTTTAACCAATTGTGCAGTTG
TGAATGAAAAGGATTTCCAGTCTGGCCAGCATGTGATTGTGAGGACCTCTCCCAATCACAGGTACACATT
TACACTGAAGACACATCCATCGGTGGTTCCAGGGAGCATTGCATTCAGTTTACCTCAGAGAAAATGGGCT
GGGCTTTCTATTGGGCAAGAAATAGAAGTCTCCTTATATACATTTGACAAAGCCAAACAGTGTATTGGCA
CAATGACCATCGAGATTGATTTCCTGCAGAAAAAAAGCATTGACTCCAACCCTTATGACACCGACAAGAT
GGCAGCAGAATTTATTCAGCAATTCAACAACCAGGCCTTCTCAGTGGGACAACAGCTTGTCTTTAGCTTC
AATGAAAAGCTTTTTGGCTTACTGGTGAAGGACATTGAAGCCATGGATCCTAGCATCCTGAAGGGAGAGC
CTGCGACAGGGAAAAGGCAGAAGATTGAAGTAGGACTGGTTGTTGGAAACAGTCAAGTTGCATTTGAAAA
AGCAGAAAATTCGTCACTTAATCTTATTGGCAAAGCTAAAACCAAGGAAAATCGCCAATCAATTATCAAT
CCTGACTGGAACTTTGAAAAAATGGGAATAGGAGGTCTAGACAAGGAATTTTCAGATATTTTCCGACGAG
CATTTGCTTCCCGAGTATTTCCTCCAGAGATTGTGGAGCAGATGGGTTGTAAACATGTTAAAGGCATCCT
GTTATATGGACCCCCAGGTTGTGGTAAGACTCTCTTGGCTCGACAGATTGGCAAGATGTTGAATGCAAGA
GAGCCCAAAGTGGTCAATGGGCCAGAAATCCTTAACAAATATGTGGGAGAATCAGAGGCTAACATTCGCA
AACTTTTTGCTGATGCTGAAGAGGAGCAAAGGAGGCTTGGTGCTAACAGTGGTTTGCACATCATCATCTT
TGATGAAATTGATGCCATCTGCAAGCAGAGAGGGAGCATGGCTGGTAGCACGGGAGTTCATGACACTGTT
GTCAACCAGTTGCTGTCCAAAATTGATGGCGTGGAGCAGCTAAACAACATCCTAGTCATTGGAATGACCA
ATAGACCAGATCTGATAGATGAGGCTCTTCTTAGACCTGGAAGACTGGAAGTTAAAATGGAGATAGGCTT
GCCAGATGAGAAAGGCCGACTACAGATTCTTCACATCCACACAGCAAGAATGAGAGGGCATCAGTTACTC
TCTGCTGATGTAGACATTAAAGAACTGGCCGTGGAGACCAAGAATTTCAGTGGTGCTGAATTGGAGGGTC
TAGTGCGAGCAGCCCAGTCCACTGCTATGAATAGACACATAAAGGCCAGTACTAAAGTGGAAGTGGACAT
GGAGAAAGCAGAAAGCCTGCAAGTGACGAGAGGAGACTTCCTTGCTTCTTTGGAGAATGATATCAAACCA
GCCTTTGGCACAAACCAAGAAGATTATGCAAGTTACATTATGAACGGTATCATCAAATGGGGTGACCCAG
TTACTCGAGTTCTAGATGATGGGGAGCTGCTGGTGCAGCAGACTAAGAACAGTGACCGCACACCATTGGT
CAGCGTGCTTCTGGAAGGCCCTCCTCACAGTGGGAAGACTGCTTTAGCTGCAAAAATTGCAGAGGAATCC
AACTTCCCATTCATCAAGATCTGTTCTCCTGATAAAATGATTGGCTTTTCTGAAACAGCCAAATGTCAGG
CCATGAAGAAGATCTTTGATGATGCGTACAAATCCCAGCTCAGTTGTGTGGTTGTGGATGACATTGAGAG
ATTGCTTGATTACGTCCCTATTGGCCCTCGATTTTCAAATCTTGTATTACAGGCTCTTCTCGTTTTACTG
AAAAAGGCACCTCCTCAGGGCCGCAAGCTTCTTATCATTGGGACCACTAGCCGCAAAGATGTCCTTCAGG
AGATGGAAATGCTTAACGCTTTCAGCACCACCATCCACGTGCCCAACATTGCCACAGGAGAGCAGCTGTT
GGAAGCTTTGGAGCTTTTGGGCAACTTCAAGGATAAGGAACGCACCACAATTGCACAGCAAGTCAAAGGG
AAGAAGGTCTGGATAGGAATCAAGAAGTTACTAATGCTGATCGAGATGTCCCTACAGATGGATCCTGAAT
ACCGTGTGAGAAAATTCTTGGCCCTCTTAAGAGAAGAAGGAGCTAGCCCCCTTGATTTTGAT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG205983 representing NM_006178
Red=Cloning site Green=Tags(s)

MAGRSMQAARCPTDELSLTNCAVVNEKDFQSGQHVIVRTSPNHRYTFTLKTHPSVVPGSIAFSLPQRKWA
GLSIGQEIEVSLYTFDKAKQCIGTMTIEIDFLQKKSIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSF
NEKLFGLLVKDIEAMDPSILKGEPATGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIIN
PDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAR
EPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTV
VNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLL
SADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKP
AFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEES
NFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLL
KKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFSTTIHVPNIATGEQLLEALELLGNFKDKERTTIAQQVKG
KKVWIGIKKLLMLIEMSLQMDPEYRVRKFLALLREEGASPLDFD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006178
ORF Size 2232 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006178.1, NP_006169.1
RefSeq Size 3960 bp
RefSeq ORF 2235 bp
Locus ID 4905
UniProt ID P46459
Cytogenetics 17q21.31
Domains AAA, cdc48_2, cdc48_N
Protein Families Protease
Summary Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity).UniProtKB/Swiss-Prot Function
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"NM_006178" in other vectors (6)
SKU Description Size Price
RC205983 NSF (Myc-DDK-tagged)-Human N-ethylmaleimide-sensitive factor (NSF) 10 ug
$681.00
RC205983L1 Lenti ORF clone of Human N-ethylmaleimide-sensitive factor (NSF), Myc-DDK-tagged 10 ug
$981.00
RC205983L2 Lenti ORF clone of Human N-ethylmaleimide-sensitive factor (NSF), mGFP tagged 10 ug
$981.00
RC205983L3 Lenti ORF clone of Human N-ethylmaleimide-sensitive factor (NSF), Myc-DDK-tagged 10 ug
$981.00
RC205983L4 Lenti ORF clone of Human N-ethylmaleimide-sensitive factor (NSF), mGFP tagged 10 ug
$981.00
SC116292 NSF (untagged)-Human N-ethylmaleimide-sensitive factor (NSF) 10 ug
$682.00

Plasmid Map for RG205983

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG205983
Circular map for RG205983

file-alt Citations

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