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EFHC2 (NM_025184) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG205975
EFHC2 (tGFP-tagged) - Human EF-hand domain (C-terminal) containing 2 (EFHC2)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$789.00 $886.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EFHC2
Synonyms dJ1158H2.1; MRX74
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG205975 representing NM_025184
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCTGCCTCTGCTGCCGGGCAACAGCTTCAACCGCAACGTGGGAAAGGAGAAGTTTCACAAATCCC
AACATTGGGGCTTTTGCAACAATGTTATGATGTTGGTGAGTGATGAAAAGCCTGGAATAGGTGGAGAACC
ACTTCTGGGGCAAAAGATAAAGCCTAAATGTAGCATATATCCTAAAGGAGATGGAAGTGATGTACCATCA
TGGGTAGCCTTTGATAAACAGGTATTATCTTTTGATGCCTATTTGGAAGAGGAAGTACTTGATAAAAGCC
AAACCAACTACAGAATAAGATACTATAAAATCTACTTCTACCCTGAAGATGACACAATTCAAGTAAATGA
ACCAGAGGTGAAAAATAGTGGATTACTTCAAGGGACTTCTATCCGGCGTCATCGGATTACTCTTCCGCCT
CCTGATGAGGATCAGTTTTATACTGTGTATCATTTTAATGTCGGCACAGAGGTTGTCTTCTATGGCCGGA
CATTCAAGATTTATGACTGTGATGCATTCACAAGAAACTTTTTGAGGAAAATAGGGGTCAAAGTGAATCC
CCCAGTGCAATGTCCAGAAGATCCTTACATGAAGATTCGGAGAGAGGTTGTAGAACACGTAGAGCCCTTA
CGTCCCTACGAATCCCTCGACACCCTGAAACAGTTCCTCCAGTATCATGGCAAGATTTTGTGTTTCTTCT
GCCTGTGGGATGACTCAGTCTCAATGTTTGGAGACCGTAGAGAACTCATCCTGCATTACTTCTTGTGTGA
TGATACTATTGAAATCAAAGAATTGCTTCCACACAGCTCAGGCCGAGATGCTCTAAAAATGTTCCTCCGG
AGGAGTAAGCTACCCAAGAATTGCCCACCTAGAGTCTATCAACCAGGCCAGATAACAGATCGAGCAGTTC
TCAATTCATATGGTGACTTTATAAAGAACCAAGCGGATGGCTACCTGTTCGATAGATATAAGCTAGGAAA
AGTAGACCAAGAGTTTTACAAAGATAGTGACCTGTCCCTAGGAGTCACCATCAATGTGTGGGGAAGAAAA
GTGCTCCTTTATGACTGTGATGAATTTACGAAGTCTTATTATAAGTCTAAATATGGAATTGAGAACTTTA
CCTCAGTTTCATGCAAGCCTCCTTCTCCTCCTCCAAAAATAGAAAGGAAATTTCCACCTTACAACGGTTT
TGGTTCTGAAGAGGATTCTCTCCGTAACTGCATAGACCTCAAGCCCACACCTCATCGGAGGAACTTCAAG
AAGTTTATGGAAAAAGACAGCTATGGCTCCAAAAGCAATATACTCCGTTTTTTTGCAAAACTAGTCACAG
ACAAATGTGTTGACTTGGACAGGATGTTTGTTATTTCATATTATCTCGGTGATGACACCATTTCAGTGTT
TGAACCTATAGAGAGGAATTCAGGAATTGCTGGTGGGATGTTCTTGAAAAGAAGTCGCGTTAAGAAGCCT
GGACAAGAAGTCTTTAAAAGTGAACTATCTGAATATATCAAGGCCGAGGAGCTGTACATTGGAGTCACGG
TGAATGTGAATGGTTACCTATTTCGTTTGCTCAATGCTGATGAGTATACCTTAAACTACATGGAGCAGAA
TACAGATAAGTATCCTTTCAGTAACCTCAAACTTGCCCTACAAAAGCTGAAGCAAGAAGAAGGAAAATCC
AGAGAGCTCAAGCAGGTATTTAAAGCTGCTGACTCTAAGCACACAAATATGGTGGATTATAATACATTCA
GAGACATATTGATGTCTTTGACTGTTGGAAACCTTGCAGAGCAAGAATTTGTAACCATTGCACGTCACTA
CCGTGTGCCTGAGGGCACATGTTCAGATATGGATTTCTTAATCGCACTGGCCCACGAAAAGTTCAAGAAA
AATATGTTTGAGAATTTCGACACTTTCATTTATTCCTGTGTGTATGAAGATCGAGAAAAAAAAAATGTAT
TACCCACCAAAGACATTAAAAGGCTGTGCAAATCCTCCAGATTACCTTTGAGTGATGATCTTCTAGAATC
CTTATTGTCAAGGTTTGAAGACAGTGAAAAACAAATAGATTATAAGTCATTTTTCTCTGCCCTGAACTGG
AGAAAGAATCCAGTGCCTGAATTGCAACCAGCATCATACCTTAAAGAGAGATGTGAAGATGTTTGGCTTG
GTATGCCATCACCTATTCCTGCGAAATACATTGACTACTGGACCTTTTTGAAGGACGCGTTTGGCTTAGA
GGAGGAA

AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG205975 representing NM_025184
Red=Cloning site Green=Tags(s)

MALPLLPGNSFNRNVGKEKFHKSQHWGFCNNVMMLVSDEKPGIGGEPLLGQKIKPKCSIYPKGDGSDVPS
WVAFDKQVLSFDAYLEEEVLDKSQTNYRIRYYKIYFYPEDDTIQVNEPEVKNSGLLQGTSIRRHRITLPP
PDEDQFYTVYHFNVGTEVVFYGRTFKIYDCDAFTRNFLRKIGVKVNPPVQCPEDPYMKIRREVVEHVEPL
RPYESLDTLKQFLQYHGKILCFFCLWDDSVSMFGDRRELILHYFLCDDTIEIKELLPHSSGRDALKMFLR
RSKLPKNCPPRVYQPGQITDRAVLNSYGDFIKNQADGYLFDRYKLGKVDQEFYKDSDLSLGVTINVWGRK
VLLYDCDEFTKSYYKSKYGIENFTSVSCKPPSPPPKIERKFPPYNGFGSEEDSLRNCIDLKPTPHRRNFK
KFMEKDSYGSKSNILRFFAKLVTDKCVDLDRMFVISYYLGDDTISVFEPIERNSGIAGGMFLKRSRVKKP
GQEVFKSELSEYIKAEELYIGVTVNVNGYLFRLLNADEYTLNYMEQNTDKYPFSNLKLALQKLKQEEGKS
RELKQVFKAADSKHTNMVDYNTFRDILMSLTVGNLAEQEFVTIARHYRVPEGTCSDMDFLIALAHEKFKK
NMFENFDTFIYSCVYEDREKKNVLPTKDIKRLCKSSRLPLSDDLLESLLSRFEDSEKQIDYKSFFSALNW
RKNPVPELQPASYLKERCEDVWLGMPSPIPAKYIDYWTFLKDAFGLEEE

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_025184
ORF Size 2247 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025184.4
RefSeq Size 2511 bp
RefSeq ORF 2250 bp
Locus ID 80258
UniProt ID Q5JST6
Cytogenetics Xp11.3
Domains DM10, EFh
Summary This gene encodes a protein which contains three DM10 domains and three calcium-binding EF-hand motifs. A related protein is encoded by a gene on chromosome 6. It has been suggested that both proteins are involved in the development of epilepsy (PMID: 15258581, 16112844) and that this gene may be associated with fear recognition in individuals with Turner syndrome. provided by RefSeq, Aug 2011
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"NM_025184" in other vectors (4)
SKU Description Size Price
RC205975 EFHC2 (Myc-DDK-tagged)-Human EF-hand domain (C-terminal) containing 2 (EFHC2) 10 ug
$686.00
RC205975L3 Lenti ORF clone of Human EF-hand domain (C-terminal) containing 2 (EFHC2), Myc-DDK-tagged 10 ug
$986.00
RC205975L4 Lenti ORF clone of Human EF-hand domain (C-terminal) containing 2 (EFHC2), mGFP tagged 10 ug
$986.00
SC110236 EFHC2 (untagged)-Human EF-hand domain (C-terminal) containing 2 (EFHC2) 10 ug
$687.00

Plasmid Map for RG205975

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG205975
Circular map for RG205975

file-alt Citations

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