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NUAK2 (NM_030952) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG205740
NUAK2 (tGFP-tagged) - Human NUAK family, SNF1-like kinase, 2 (NUAK2)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
$489.00 $785.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NUAK2
Synonyms SNARK
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG205740 representing NM_030952
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCGCTGGTTTTCGCGCGGCGCTCCGGCCCCACTCCCTCGGCCGCAGAGCTAGCCCGGCCGCTGG
CGGAAGGGCTGATCAAGTCGCCCAAGCCCCTAATGAAGAAGCAGGCGGTGAAGCGGCACCACCACAAGCA
CAACCTGCGGCACCGCTACGAGTTCCTGGAGACCCTGGGCAAAGGCACCTACGGGAAGGTGAAGAAGGCG
CGGGAGAGCTCGGGGCGCCTGGTGGCCATCAAGTCAATCCGGAAGGACAAAATCAAAGATGAGCAAGATC
TGATGCACATACGGAGGGAGATTGAGATCATGTCATCACTCAACCACCCTCACATCATTGCCATCCATGA
AGTGTTTGAGAACAGCAGCAAGATCGTGATCGTCATGGAGTATGCCAGCCGGGGCGACCTTTATGACTAC
ATCAGCGAGCGGCAGCAGCTCAGTGAGCGCGAAGCTAGGCATTTCTTCCGGCAGATCGTCTCTGCCGTGC
ACTATTGCCATCAGAACAGAGTTGTCCACCGAGATCTCAAGCTGGAGAACATCCTCTTGGATGCCAATGG
GAATATCAAGATTGCTGACTTCGGCCTCTCCAACCTCTACCATCAAGGCAAGTTCCTGCAGACATTCTGT
GGGAGCCCCCTCTATGCCTCGCCAGAGATTGTCAATGGGAAGCCCTACACAGGCCCAGAGGTGGACAGCT
GGTCCCTGGGTGTTCTCCTCTACATCCTGGTGCATGGCACCATGCCCTTTGATGGGCATGACCATAAGAT
CCTAGTGAAACAGATCAGCAACGGGGCCTACCGGGAGCCACCTAAACCCTCTGATGCCTGTGGCCTGATC
CGGTGGCTGTTGATGGTGAACCCCACCCGCCGGGCCACCCTGGAGGATGTGGCCAGTCACTGGTGGGTCA
ACTGGGGCTACGCCACCCGAGTGGGAGAGCAGGAGGCTCCGCATGAGGGTGGGCACCCTGGCAGTGACTC
TGCCCGCGCCTCCATGGCTGACTGGCTCCGGCGTTCCTCCCGCCCCCTCCTGGAGAATGGGGCCAAGGTG
TGCAGCTTCTTCAAGCAGCATGCACCTGGTGGGGGAAGCACCACCCCTGGCCTGGAGCGCCAGCATTCGC
TCAAGAAGTCCCGCAAGGAGAATGACATGGCCCAGTCTCTCCACAGTGACACGGCTGATGACACTGCCCA
TCGCCCTGGCAAGAGCAACCTCAAGCTGCCAAAGGGCATTCTCAAGAAGAAGGTGTCAGCCTCTGCAGAA
GGGGTACAGGAGGACCCTCCGGAGCTCAGCCCAATCCCTGCGAGCCCAGGGCAGGCTGCCCCCCTGCTCC
CCAAGAAGGGCATTCTCAAGAAGCCCCGACAGCGCGAGTCTGGCTACTACTCCTCTCCCGAGCCCAGTGA
ATCTGGGGAGCTCTTGGACGCAGGCGACGTGTTTGTGAGTGGGGATCCCAAGGAGCAGAAGCCTCCGCAA
GCTTCAGGGCTGCTCCTCCATCGCAAAGGCATCCTCAAACTCAATGGCAAGTTCTCCCAGACAGCCTTGG
AGCTCGCGGCCCCCACCACCTTCGGCTCCCTGGATGAACTCGCCCCACCTCGCCCCCTGGCCCGGGCCAG
CCGACCCTCAGGGGCTGTGAGCGAGGACAGCATCCTGTCCTCTGAGTCCTTTGACCAGCTGGACTTGCCT
GAACGGCTCCCAGAGCCCCCACTGCGGGGCTGTGTGTCTGTGGACAACCTCACGGGGCTTGAGGAGCCCC
CCTCAGAGGGCCCTGGAAGCTGCCTGAGGCGCTGGCGGCAGGATCCTTTGGGGGACAGCTGCTTTTCCCT
GACAGACTGCCAGGAGGTGACAGCGACCTACCGACAGGCACTGAGGGTCTGCTCAAAGCTCACC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG205740 representing NM_030952
Red=Cloning site Green=Tags(s)

MESLVFARRSGPTPSAAELARPLAEGLIKSPKPLMKKQAVKRHHHKHNLRHRYEFLETLGKGTYGKVKKA
RESSGRLVAIKSIRKDKIKDEQDLMHIRREIEIMSSLNHPHIIAIHEVFENSSKIVIVMEYASRGDLYDY
ISERQQLSEREARHFFRQIVSAVHYCHQNRVVHRDLKLENILLDANGNIKIADFGLSNLYHQGKFLQTFC
GSPLYASPEIVNGKPYTGPEVDSWSLGVLLYILVHGTMPFDGHDHKILVKQISNGAYREPPKPSDACGLI
RWLLMVNPTRRATLEDVASHWWVNWGYATRVGEQEAPHEGGHPGSDSARASMADWLRRSSRPLLENGAKV
CSFFKQHAPGGGSTTPGLERQHSLKKSRKENDMAQSLHSDTADDTAHRPGKSNLKLPKGILKKKVSASAE
GVQEDPPELSPIPASPGQAAPLLPKKGILKKPRQRESGYYSSPEPSESGELLDAGDVFVSGDPKEQKPPQ
ASGLLLHRKGILKLNGKFSQTALELAAPTTFGSLDELAPPRPLARASRPSGAVSEDSILSSESFDQLDLP
ERLPEPPLRGCVSVDNLTGLEEPPSEGPGSCLRRWRQDPLGDSCFSLTDCQEVTATYRQALRVCSKLT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030952
ORF Size 1884 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030952.1, NP_112214.1
RefSeq Size 3443 bp
RefSeq ORF 1887 bp
Locus ID 81788
UniProt ID Q9H093
Cytogenetics 1q32.1
Protein Families Druggable Genome, Protein Kinase
Summary Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1.UniProtKB/Swiss-Prot Function
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"NM_030952" in other vectors (7)
SKU Description Size Price
RC205740 NUAK2 (Myc-DDK-tagged)-Human NUAK family, SNF1-like kinase, 2 (NUAK2) 10 ug
$585.00
RC205740L1 Lenti ORF clone of Human NUAK family, SNF1-like kinase, 2 (NUAK2), Myc-DDK-tagged 10 ug
$885.00
RC205740L2 Lenti ORF clone of Human NUAK family, SNF1-like kinase, 2 (NUAK2), mGFP tagged 10 ug
$885.00
RC205740L3 Lenti ORF clone of Human NUAK family, SNF1-like kinase, 2 (NUAK2), Myc-DDK-tagged 10 ug
$885.00
RC205740L4 Lenti ORF clone of Human NUAK family, SNF1-like kinase, 2 (NUAK2), mGFP tagged 10 ug
$885.00
SC109313 NUAK2 (untagged)-Human NUAK family, SNF1-like kinase, 2 (NUAK2) 10 ug
$585.00
SC323459 NUAK2 (untagged)-Kinase deficient mutant (K81M) of Human NUAK family, SNF1-like kinase, 2 (NUAK2) 10 ug
$585.00

Plasmid Map for RG205740

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG205740
Circular map for RG205740

file-alt Citations

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