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SWT1 (NM_017673) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG205449
SWT1 (tGFP-tagged) - Human SWT1 RNA endoribonuclease homolog (S. cerevisiae) (SWT1), transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $1,024.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SWT1
Synonyms C1orf26; HsSwt1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG205449 representing NM_017673
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCAGCAAAGAATCCTGTGGGAAAAAAGAGACATCTCAGAGGAAAGACACCACCACCTCATCACCCA
ATTTTGGTGAAAAAGACAAGAAAGAGAGAAAAACCCCAGCAAGTTCTACTAGTTCGTCTTCTATAAGATC
AGTTTCATCAGAAAAGAGAAAACTGAAATCAGATCATACAGATGTTCTGTACTATAATATAAAAAGAAGA
CAAGGACTGAAAAGATTGAGTGTAGAAATTGACACTCTCAGAAGGAGACCAAAAATCGGTTCTTCATCCC
AAAGACCTATTAAACTCAAAGAAGCATCATATTCAAATGATAATCAAATTATTTTGCAGAGTCCTTCTTC
AAATGGAACTAAAAAAGACATACATAAATGTGTAGACTTTAAACCTAAAGATATCAAATTGACAAATGCT
GGGAGCAAGCTTGACCATGGAGTTAAAAGCCTTAGTAGTCCTAAGATTGCCAGTGATGTGAAACCTAAAG
CCGAAGGCCAGGCAAGTGAAAATAAATGGTCTCATTTACTTGTTCAGAGAGAGAAGATGAAAGAACTCAA
GAAAGGAAGAAACAGTAAATTTAGAGACAATTCTGAAAAATGTGTCTTAGAGAAATGGAAGAGAAATCAA
TTTTCTCAGGATTATAACTCCAACAAGATAATTAAGGAACCCTTGGGATCTAGGAGACAGAAGATCAGTT
TCAAAATCCCTATAAAATCCCGTGACACCCTCCAGAAACTTGTAGAAGAAAATGTCTTCAACATAGATTC
TAATAATTCGAAGACTAAGCAGGAAGAAAGAGAATACCTGGAAAGCTCCCAGGTTTCATTAAATGTGACT
AGGCAGAAAACTGAACATTTACTTTCAGATTTTACATATAAGCGGACTGTTCATGAGTGGAAACGAAAAC
ATCATTATGACCATCAAGAAAGTAATGATTCACATTCTAGGGAAAACCTAACCCAGAGTTTTGAAGCACC
ATGTTGTTCCGTGTCATCTGAAAGTATCCAGGATGCAGATCAAGAGATGCAGATAGTAGAAGAGCTTCAT
GCTGCACGTGTGGGAAAAAGTGTGGATTTACCTGGAGAGTTAATGAGTATGGAAATTGACTTAGAAGATG
ATGTACATTCCTCCTCTGCAAATAATACTTCAGACAGAAAGCTTCTAATTGTTATTGACACAAATATTCT
GATGAATCATCTCAAATTTGTTAGAATTTTGAAGACAACAGAAGTACCAGGTTTTGACAAACTTGTGTTA
ATAATTCCCTGGGTCGTTATGCAAGAGCTAGATCGTATGAAGGAAGGAAAACTACTAAAACGTGCCCAGC
ACAAAGCTATACCTGCAGTTCATTTCATCAACGACAGTCTCAAAAATCAAGATAGAAAGCTATGGGGTCA
GTCAATACAACTTGCATCCCAAAAACATTATGGATTGAGTGATGAGAACAATGATGATCGAGTACTAAAA
TGCTGTCTCCAGCACCAGGAATTATTCCCTTGTTCTTTTGTTATTCTGTGCACGGATGATAGAAACTTAA
GAAACAAAGGCCTGATAAGTGGTGTGAAGTCACTCAGTAAAGAAGAATTGAGTGCAGAGTTATTACGCTT
ATCTCTGAACACAGATGTGTGTCATCAGCCTTGTATTCCTAAGCAACAGTTGAAAGCAGAAACAACACCC
TTGAAAGAGAGCTATAAGGAGGAATCTACAAGTTCTGGACTGTCCATTCTGCTTGAGAGCATTGTATCTG
ATCTTGAAAAATCTCTTGGAACAGGTTTATCTTCAATATTAGAAACAGAAATGAAAATTGCTTTTGGAAA
CCTTTGGATGGAGATCCTGTACCTGAAACCACCATGGACTCTACTACATTTACTACAGTGCTTTAAAAAA
CATTGGTTGGCTGTATTTGGATTAGTTATGGAAAAGAACTTGCTTTTAACTATTGAGAGCCTATACAAAA
ATCTCCGTAAAGCTAATAAGGCAGTGGATTTTACAACAGTCAAATTCTTGCTTCAGGATTCTAGAAGTTT
GTTACATGCTTTCAGTACAAGGTCAAATTATGATGGTATTCTTCCACAGACCTTTGCTCAAGTAAACAAC
CTCCTTCAGACATTTGCAGAGGTCAAGACAAAACTTAAGCCAAATTCTTCAGAAAACACAGTGACTAAAA
AGCAGGAAGGTACTTCATTGAAGAATTCTCATAATCAAGAAATCACTGTTTTCTCGAGTTCTCATCTTCC
CCAACCCAGCAGGCATCAAGAAATCTGGTCTATCCTAGAGAGTGTTTGGATTACAATATATCAGAACAGC
ACGGATGTATTTCAAAGATTGGGCTCAAATTCAGCTCTGACTACTTCAAATATAGCATCATTTGAAGAAG
CATTTATATGTCTTCAAAAGTTAATGGCAGCTGTGAGGGATATTCTTGAAGGAATTCAAAGGATTTTGGC
CCCAAACAGTGATTATCAAGATGTTGAGACCCTCTATAACTTCCTAATCAAGTATGAGGTAAATAAAAAT
GTCAAATTTACTGCCCAGGAAATTTATGATTGTGTTTCTCAGACTGAGTATAGGGAAAAGTTAACCATTG
GATGCCGCCAGCTGGTTGAGATGGAATATACCATGCAGCAGTGCAATGCATCTGTTTATATGGAGGCCAA
AAACAGGGGATGGTGTGAAGACATGCTCAACTATAGGATA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG205449 representing NM_017673
Red=Cloning site Green=Tags(s)

MSSKESCGKKETSQRKDTTTSSPNFGEKDKKERKTPASSTSSSSIRSVSSEKRKLKSDHTDVLYYNIKRR
QGLKRLSVEIDTLRRRPKIGSSSQRPIKLKEASYSNDNQIILQSPSSNGTKKDIHKCVDFKPKDIKLTNA
GSKLDHGVKSLSSPKIASDVKPKAEGQASENKWSHLLVQREKMKELKKGRNSKFRDNSEKCVLEKWKRNQ
FSQDYNSNKIIKEPLGSRRQKISFKIPIKSRDTLQKLVEENVFNIDSNNSKTKQEEREYLESSQVSLNVT
RQKTEHLLSDFTYKRTVHEWKRKHHYDHQESNDSHSRENLTQSFEAPCCSVSSESIQDADQEMQIVEELH
AARVGKSVDLPGELMSMEIDLEDDVHSSSANNTSDRKLLIVIDTNILMNHLKFVRILKTTEVPGFDKLVL
IIPWVVMQELDRMKEGKLLKRAQHKAIPAVHFINDSLKNQDRKLWGQSIQLASQKHYGLSDENNDDRVLK
CCLQHQELFPCSFVILCTDDRNLRNKGLISGVKSLSKEELSAELLRLSLNTDVCHQPCIPKQQLKAETTP
LKESYKEESTSSGLSILLESIVSDLEKSLGTGLSSILETEMKIAFGNLWMEILYLKPPWTLLHLLQCFKK
HWLAVFGLVMEKNLLLTIESLYKNLRKANKAVDFTTVKFLLQDSRSLLHAFSTRSNYDGILPQTFAQVNN
LLQTFAEVKTKLKPNSSENTVTKKQEGTSLKNSHNQEITVFSSSHLPQPSRHQEIWSILESVWITIYQNS
TDVFQRLGSNSALTTSNIASFEEAFICLQKLMAAVRDILEGIQRILAPNSDYQDVETLYNFLIKYEVNKN
VKFTAQEIYDCVSQTEYREKLTIGCRQLVEMEYTMQQCNASVYMEAKNRGWCEDMLNYRI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017673
ORF Size 2700 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017673.5
RefSeq Size 3770 bp
RefSeq ORF 2703 bp
Locus ID 54823
UniProt ID Q5T5J6
Cytogenetics 1q25.3
Domains PINc
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Documents
Resources
"NM_017673" in other vectors (4)
SKU Description Size Price
RC205449 SWT1 (Myc-DDK-tagged)-Human SWT1 RNA endoribonuclease homolog (S. cerevisiae) (SWT1), transcript variant 1 10 ug
$824.00
RC205449L3 Lenti ORF clone of Human SWT1 RNA endoribonuclease homolog (S. cerevisiae) (SWT1), transcript variant 1, Myc-DDK-tagged 10 ug
$1,124.00
RC205449L4 Lenti ORF clone of Human SWT1 RNA endoribonuclease homolog (S. cerevisiae) (SWT1), transcript variant 1, mGFP tagged 10 ug
$1,124.00
SC114043 SWT1 (untagged)-Human SWT1 RNA endoribonuclease homolog (S. cerevisiae) (SWT1), transcript variant 1 10 ug
$825.00

Plasmid Map for RG205449

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG205449
Circular map for RG205449

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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