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RNF113B (NM_178861) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG205407
RNF113B (tGFP-tagged) - Human ring finger protein 113B (RNF113B)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $500.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RNF113B
Synonyms bA10G5.1; RNF161; ZNF183L1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG205407 representing NM_178861
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCGCCACCTTCTCCAGGAAGGACGGCCGACCAAGCAGACCAGGTATGCACCTTCCTCTTCAAAA
AGCCTGGACGGAAAGGGGCTGCAGGCCTCAGAAAGCGCCCGGCCTGCGACCCCGAGCACGGAGAGAGCAG
CAGCAGCGGGGACGAGGGCGACACAGTGGCTCAGCCCCCGCGGGTGGCACCGAGGCCCCGGGGCCTCCAC
AGCTGGCAGAAGGCGGCTCACGGCGACAGGAGGGGCGAGGAGGCGGCGCCTGAGAGCCTCGACGTGGTGT
ACAGGTCCACCCGCTCGGCGAAGCCTGTGGGGCCGGAGGACATGGGGGCCACCGCTGACTTCGAGCAGGA
CACCGAGAAGGAGCACCATACGCCGACCATCCTCAAGTGCAGCCAGCGGGTCCAGGAGGCACTGCGGGGT
CGGGAGCACGACCACATCTACCGGGGAATCCACAGCTACCTGAGGTACCTGAAGCCCAAGGACACGTCCA
TGGGCAACTCCTCCTCGGGGATGGCGAGGAAGGGCCCCATACGTGCGCCAGGGCATCTGCGCGCCACTGT
GCGCTGGGATTACCAGCCTGACATCTGCAAGGACTACAAGGAGACTGGCTTCTGTGGCTTCGGGGACAGC
TGCAAATTCCTCCACGACCGTTCCGATTACAAGCTCGGGTGGGAGATTGAACGGGAGCTTGAAGAGGGTC
GCTACTGTATCTGCGAGGACGAAAACCATGAAGTGGGAAGCGAGGAAGAGGAAATACCATTCAGGTGTTT
CATATGTCGCCAGGCCTTCCAAAACCCAGTCGTCACCAAGTGCAGGCATTATTTCTGCGAGAGCTGCGCG
CTGGAGCACTTCCGGGCCACCCCGCGCTGCTACATCTGTGACCAGCCAACCGGCGGCATCTTTAACCCCG
CCAAAGAACTGATGGCGAAACTGCAAAAGCTTCAGGCTGCAGAAGGAAAAAAGAGA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG205407 representing NM_178861
Red=Cloning site Green=Tags(s)

MAAPPSPGRTADQADQVCTFLFKKPGRKGAAGLRKRPACDPEHGESSSSGDEGDTVAQPPRVAPRPRGLH
SWQKAAHGDRRGEEAAPESLDVVYRSTRSAKPVGPEDMGATADFEQDTEKEHHTPTILKCSQRVQEALRG
REHDHIYRGIHSYLRYLKPKDTSMGNSSSGMARKGPIRAPGHLRATVRWDYQPDICKDYKETGFCGFGDS
CKFLHDRSDYKLGWEIERELEEGRYCICEDENHEVGSEEEEIPFRCFICRQAFQNPVVTKCRHYFCESCA
LEHFRATPRCYICDQPTGGIFNPAKELMAKLQKLQAAEGKKR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178861
ORF Size 966 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178861.3
RefSeq Size 1381 bp
RefSeq ORF 969 bp
Locus ID 140432
UniProt ID Q8IZP6
Cytogenetics 13q32.2
Protein Families Druggable Genome
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Documents
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"NM_178861" in other vectors (4)
SKU Description Size Price
RC205407 RNF113B (Myc-DDK-tagged)-Human ring finger protein 113B (RNF113B) 10 ug
$300.00
RC205407L3 Lenti ORF clone of Human ring finger protein 113B (RNF113B), Myc-DDK-tagged 10 ug
$600.00
RC205407L4 Lenti ORF clone of Human ring finger protein 113B (RNF113B), mGFP tagged 10 ug
$600.00
SC307236 RNF113B (untagged)-Human ring finger protein 113B (RNF113B) 10 ug
$330.00

Plasmid Map for RG205407

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for RG205407
Circular map for RG205407

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