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ACTL8 (NM_030812) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG205140
ACTL8 (tGFP-tagged) - Human actin-like 8 (ACTL8)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $657.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ACTL8
Synonyms CT57
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG205140 representing NM_030812
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTCAAGAACCGTTATCATTGACCACGGGTCTGGCTTTTTGAAGGCTGGCACGGCCGGATGGAATG
AGCCTCAGATGGTCTTCCCGAACATCGTGAACTACCTACCGTGCAAGGAGAACCCTGGCCCCAGCTATGC
CCGTAGGCGTGTGAGCCTGGGCATCGACATTTGCCATCCTGACACCTTTAGCTACCCCATCGAGCGGGGC
CGCATCCTCAACTGGGAGGGTGTGCAGTACCTCTGGTCATTTGTGTTGGAGAACCACAGACGGGAGCAAG
AGGTCCCCCCTGTGATCATCACGGAGACACCCTTGAGGGAGCCTGCGGACCGAAAGAAGATGCTGGAGAT
CCTGTTTGAGTTGCTGCATGTCCCATCGGTCCTCCTGGCCGACCAGCTGCAGATGTCCCTGTATGCCTCT
GGCCTCCTGACCGGAGTGGTGGTTGATTCTGGCTATGGCCTGACCCGCGTGCAGCCTTTCCACCAGGGCC
GCCCCTTGCCCGCCAGCGGCAAGACGCTGGAGTTCGCCGGCCAGGATCTCTCCGCCTATCTCCTCAAGAG
TCTCTTTAAGGAAGATTGCGATAGACGCTGCCTGTTTCAGCTGGAGACAGTCGCCGTGACTCAGATGAAC
AAGTGCTACGTGCCGCAGAATCTGGGGGAGGCCCTGGACTTTCGTGAGAGGCAGCAGAGTGCCTTGGATG
AGAGCAACACCTATCAGCTCCCAGACGGCTCCCGCGTGGAGCTGACCCCCATGCAGCGGGTGGCTCCTGA
GATGTTCTTTAGCCCGCAGGTGTTCGAGCAGCCGGGGCCCAGCATCCCACGGGCCATTGTGGAATCCGTG
GAGTCCTGCGAGATCTCCCTGCGCCCCCTGCTGGTCTCCCACGTGATGGCCTGCGGGGGCAACACCCTCT
ATCCCGGGTTCACAAAGCGCCTGTTCAGGGAGCTGATGGGGGATCACGTCTCCTCCACCAAGGCCACAGT
CTGGGAGGGTTCCAATAGAAACTTTAGTGTCTGGCTAGGAGCGTCCGTGGTGGCTCACCTTTCTACCTAC
CAGTCTGAGTGGATGTCCCGAGAGGAGTATGGTGAGCATATGAGGATG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG205140 representing NM_030812
Red=Cloning site Green=Tags(s)

MASRTVIIDHGSGFLKAGTAGWNEPQMVFPNIVNYLPCKENPGPSYARRRVSLGIDICHPDTFSYPIERG
RILNWEGVQYLWSFVLENHRREQEVPPVIITETPLREPADRKKMLEILFELLHVPSVLLADQLQMSLYAS
GLLTGVVVDSGYGLTRVQPFHQGRPLPASGKTLEFAGQDLSAYLLKSLFKEDCDRRCLFQLETVAVTQMN
KCYVPQNLGEALDFRERQQSALDESNTYQLPDGSRVELTPMQRVAPEMFFSPQVFEQPGPSIPRAIVESV
ESCEISLRPLLVSHVMACGGNTLYPGFTKRLFRELMGDHVSSTKATVWEGSNRNFSVWLGASVVAHLSTY
QSEWMSREEYGEHMRM

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030812
ORF Size 1098 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030812.1, NP_110439.1
RefSeq Size 1852 bp
RefSeq ORF 1101 bp
Locus ID 81569
UniProt ID Q9H568
Cytogenetics 1p36.13
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"NM_030812" in other vectors (6)
SKU Description Size Price
RC205140 ACTL8 (Myc-DDK-tagged)-Human actin-like 8 (ACTL8) 10 ug
$457.00
RC205140L1 Lenti ORF clone of Human actin-like 8 (ACTL8), Myc-DDK-tagged 10 ug
$757.00
RC205140L2 Lenti ORF clone of Human actin-like 8 (ACTL8), mGFP tagged 10 ug
$757.00
RC205140L3 Lenti ORF clone of Human actin-like 8 (ACTL8), Myc-DDK-tagged 10 ug
$757.00
RC205140L4 Lenti ORF clone of Human actin-like 8 (ACTL8), mGFP tagged 10 ug
$757.00
SC122992 ACTL8 (untagged)-Human actin-like 8 (ACTL8) 10 ug
$457.00

Plasmid Map for RG205140

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG205140
Circular map for RG205140

file-alt Citations

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