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CDR2 (NM_001802) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG204900
CDR2 (tGFP-tagged) - Human cerebellar degeneration-related protein 2, 62kDa (CDR2)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $657.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CDR2
Synonyms CDR62; Yo
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG204900 representing NM_001802
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGGCGGAAAACCTGGTAGAGGAGTTTGAGATGAAGGAGGACGAGCCGTGGTACGACCACCAGGACC
TCCAGCAAGATCTTCAACTTGCTGCTGAGCTTGGGAAGACATTACTGGATCGGAACACAGAGTTGGAGGA
CTCTGTTCAGCAGATGTATACAACCAATCAGGAGCAGTTACAGGAAATTGAGTATCTGACGAAGCAAGTG
GAACTTCTACGGCAGATGAACGAACAACATGCAAAGGTTTATGAACAATTAGACGTCACAGCAAGGGAAC
TGGAAGAAACAAATCAAAAGCTAGTTGCTGACAGCAAGGCCTCACAGCAAAAGATTCTGAGCCTGACTGA
AACGATTGAATGCCTGCAAACCAACATTGATCACCTCCAGAGCCAAGTGGAGGAGCTGAAGTCATCTGGC
CAAGGGAGAAGGAGCCCGGGAAAGTGTGACCAGGAGAAACCGGCACCCAGCTTTGCATGTCTGAAGGAGC
TGTATGACCTCCGCCAACACTTCGTGTATGATCATGTGTTCGCTGAGAAGATCACTTCCTTGCAAGGTCA
GCCAAGCCCTGATGAAGAGGAAAATGAGCACTTGAAAAAAACAGTGACAATGTTGCAGGCCCAGCTGAGC
CTGGAGCGGCAGAAGCGGGTGACTATGGAGGAGGAATATGGGCTCGTGTTAAAGGAGAACAGTGAACTGG
AGCAGCAGCTGGGGGCCACAGGTGCCTACCGAGCACGGGCGCTGGAACTAGAGGCCGAGGTGGCAGAGAT
GCGACAGATGTTGCAGTCAGAGCATCCATTTGTGAATGGAGTTGAGAAGCTGGTGCCAGACTCTCTGTAT
GTTCCTTTCAAAGAGCCCAGCCAGAGCCTGCTGGAAGAGATGTTCCTGACTGTGCCGGAATCACATAGAA
AGCCTCTCAAGCGCAGCAGCAGTGAGACGATCCTCAGCAGCTTGGCAGGGAGTGACATCGTGAAGGGCCA
CGAGGAGACCTGCATCAGGAGGGCCAAGGCTGTGAAACAGAGGGGCATCTCCCTTCTGCACGAAGTGGAC
ACGCAGTACAGCGCCCTGAAGGTGAAGTATGAAGAGTTGCTGAAGAAGTGCCAAGAGGAACAGGACTCCC
TGTCACACAAGGCTGTGCAGACCTCCAGGGCTGCAGCCAAGGACCTGACTGGAGTGAACGCCCAGTCTGA
GCCTGTTGCCAGCGGCTGGGAACTGGCCTCTGTCAACCCAGAGCCCGTGAGTTCCCCTACAACACCTCCA
GAATACAAAGCGTTGTTTAAGGAGATCTTTAGTTGCATCAAGAAAACTAAGCAGGAAATAGATGAACAGA
GAACAAAATACCGATCACTCTCCTCTCATTCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG204900 representing NM_001802
Red=Cloning site Green=Tags(s)

MLAENLVEEFEMKEDEPWYDHQDLQQDLQLAAELGKTLLDRNTELEDSVQQMYTTNQEQLQEIEYLTKQV
ELLRQMNEQHAKVYEQLDVTARELEETNQKLVADSKASQQKILSLTETIECLQTNIDHLQSQVEELKSSG
QGRRSPGKCDQEKPAPSFACLKELYDLRQHFVYDHVFAEKITSLQGQPSPDEEENEHLKKTVTMLQAQLS
LERQKRVTMEEEYGLVLKENSELEQQLGATGAYRARALELEAEVAEMRQMLQSEHPFVNGVEKLVPDSLY
VPFKEPSQSLLEEMFLTVPESHRKPLKRSSSETILSSLAGSDIVKGHEETCIRRAKAVKQRGISLLHEVD
TQYSALKVKYEELLKKCQEEQDSLSHKAVQTSRAAAKDLTGVNAQSEPVASGWELASVNPEPVSSPTTPP
EYKALFKEIFSCIKKTKQEIDEQRTKYRSLSSHS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001802
ORF Size 1362 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001802.2
RefSeq Size 2702 bp
RefSeq ORF 1365 bp
Locus ID 1039
UniProt ID Q01850
Cytogenetics 16p12.2
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Documents
Resources
"NM_001802" in other vectors (7)
SKU Description Size Price
RC204900 CDR2 (Myc-DDK-tagged)-Human cerebellar degeneration-related protein 2, 62kDa (CDR2) 10 ug
$457.00
RC204900L1 Lenti ORF clone of Human cerebellar degeneration-related protein 2, 62kDa (CDR2), Myc-DDK-tagged 10 ug
$757.00
RC204900L2 Lenti ORF clone of Human cerebellar degeneration-related protein 2, 62kDa (CDR2), mGFP tagged 10 ug
$757.00
RC204900L3 Lenti ORF clone of Human cerebellar degeneration-related protein 2, 62kDa (CDR2), Myc-DDK-tagged 10 ug
$757.00
RC204900L4 Lenti ORF clone of Human cerebellar degeneration-related protein 2, 62kDa (CDR2), mGFP tagged 10 ug
$757.00
SC303070 CDR2 (untagged)-Human cerebellar degeneration-related protein 2, 62kDa (CDR2) 10 ug
$503.00
SC320411 CDR2 (untagged)-Human cerebellar degeneration-related protein 2, 62kDa (CDR2) 10 ug
$457.00

Plasmid Map for RG204900

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG204900
Circular map for RG204900

file-alt Citations

Paraneoplastic cerebellar degeneration: Characterizing the Yo antibody antigens
Kråkenes
Thesis (2021)
Snippet: Full-length CDR2 (OriGene Technologies, Rockville, MD, #RG204900) and CDR2L (OriGene Technologies, #RC206909) were ligated into a pCMV6-AC-GFP vector (OriGene Technologies, #PS100010)
CDR2L Is the Major Yo Antibody Target in Paraneoplastic Cerebellar Degeneration
Kråkenes, Herdlevaer, Raspotnig et al
Ann. Neurol. (2019)
AI Summary: OriGene products—full-length CDR2 (#RG204900), CDR2L (#RC206909), and the pCMV6-AC-GFP vector (#PS100010)—were used to generate recombinant proteins for transfection experiments, enabling the study to determine that CDR2L is the primary Yo antibody target in paraneoplastic cerebellar degeneration.
Snippet: All rights reserved. Page 7. Full-length CDR2 (Origene, #RG204900) and CDR2L (Origene, #RC206909) were ligated into a pCMV6-AC-GFP vector (OriGene, #PS100010). Following PCR, correct CDR2 and CDR2L vector sequences were confirmed using BioEdit
Paraneoplastic syndrome-associated neuronal antibodies in adult ADHD
Haukanes, Hegvik, Eichler et al
J Neuroimmunol (2015) 288, 87-91 DOI: 10.1016/j.jneuroim.2015.08.018
Cited by 4
AI Summary: OriGene products contributed to this study by providing the anti-Yo antibody (catalog #AM09316PU-N), which was used to investigate the presence of paraneoplastic syndrome-associated neuronal antibodies in adult ADHD patient sera.
CDR2L Antibodies: A New Player in Paraneoplastic Cerebellar Degeneration
Eichler, Totland, Haugen et al
PLoS ONE (2013) 8 (6), e66002 DOI: 10.1371/journal.pone.0066002
Cited by 57
AI Summary: OriGene’s anti-myc antibody (TA50014) was used for immunostaining experiments in this study to help detect tagged proteins, contributing to the characterization of CDR2 and CDR2L antibodies in paraneoplastic cerebellar degeneration.
Snippet: Anti myc (Origene, TA50014) was used 1:300, anti CDR2L (Biosite, ARP39113_P050) was used 1:600. ... 5). The CDR2L antibodies gave a strong band of approximately 62 kDa in a Western blot of human cerebellum extract.
WB IF
Paraneoplastic syndrome-associated neuronal antibodies in adult ADHD
Haukanes, Hegvik, Eichler et al
Journal of Neuroimmunology (2015) 288, 87-91
AI Summary: OriGene products, specifically cDNA clones for full-length CDR2 (vector RG204900) and CDR2L (vector RC206909), were used to express these proteins for antibody detection assays in the study.
Snippet: cDNA for full-length CDR2 (Origene, Germany;CDR2 sequence was transferred from vector RG204900 with C-terminal GFP into vector PS10019 with N-terminal GFP) or CDR2L (Origene;CDR2L sequence transferred from vector RC206909 with C-terminal myc-DDK ... Cite SaveSaving
Showing 1-5 of 5 papers.
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