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PYCR2 (NM_013328) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG204525
PYCR2 (tGFP-tagged) - Human pyrroline-5-carboxylate reductase family, member 2 (PYCR2)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $500.00
In Stock*
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Other products for "PYCR2 or NM_013328"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PYCR2
Synonyms HLD10; P5CR2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG204525 representing NM_013328
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGTGGGCTTCATCGGGGCCGGCCAGCTGGCCTATGCTCTGGCGCGGGGCTTCACGGCCGCAGGCA
TCCTGTCGGCTCACAAGATAATAGCCAGCTCCCCAGAAATGAACCTGCCCACGGTGTCCGCGCTCAGGAA
GATGGGTGTGAACCTGACACGCAGCAACAAGGAGACGGTGAAGCACAGCGACGTCCTGTTTCTGGCTGTG
AAGCCACATATCATCCCCTTCATCCTGGATGAGATTGGGGCCGACGTGCAAGCCAGACACATCGTGGTCT
CCTGTGCGGCTGGTGTCACCATCAGCTCTGTGGAGAAGAAGCTGATGGCATTCCAGCCAGCCCCCAAAGT
GATTCGCTGCATGACCAACACACCTGTGGTAGTGCAGGAAGGCGCTACAGTGTACGCCACGGGCACCCAT
GCCCTGGTGGAGGATGGGCAGCTCCTGGAGCAGCTCATGAGCAGCGTGGGCTTCTGCACTGAGGTGGAAG
AGGACCTCATCGATGCCGTCACGGGGCTCAGTGGCAGCGGGCCTGCCTATGCATTCATGGCTCTGGACGC
ATTGGCTGATGGTGGGGTGAAGATGGGTTTGCCACGGCGCCTGGCAATCCAACTCGGGGCCCAGGCTTTG
CTGGGAGCTGCCAAGATGCTGCTGGACTCGGAGCAGCATCCATGCCAGCTTAAGGACAATGTCTGCTCCC
CTGGGGGAGCCACCATCCACGCCCTGCACTTTCTAGAGAGTGGGGGCTTCCGCTCTCTGCTCATCAATGC
AGTTGAGGCCTCCTGTATCCGAACACGAGAGCTACAGTCCATGGCCGACCAAGAAAAGATCTCCCCAGCT
GCCCTTAAGAAGACCCTCTTAGACAGAGTGAAGCTGGAATCCCCCACAGTCTCCACACTGACCCCCTCCA
GCCCAGGGAAGCTCCTCACAAGAAGCCTGGCCCTGGGAGGCAAGAAGGAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG204525 representing NM_013328
Red=Cloning site Green=Tags(s)

MSVGFIGAGQLAYALARGFTAAGILSAHKIIASSPEMNLPTVSALRKMGVNLTRSNKETVKHSDVLFLAV
KPHIIPFILDEIGADVQARHIVVSCAAGVTISSVEKKLMAFQPAPKVIRCMTNTPVVVQEGATVYATGTH
ALVEDGQLLEQLMSSVGFCTEVEEDLIDAVTGLSGSGPAYAFMALDALADGGVKMGLPRRLAIQLGAQAL
LGAAKMLLDSEQHPCQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEASCIRTRELQSMADQEKISPA
ALKKTLLDRVKLESPTVSTLTPSSPGKLLTRSLALGGKKD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013328
ORF Size 960 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013328.4
RefSeq Size 1708 bp
RefSeq ORF 963 bp
Locus ID 29920
UniProt ID Q96C36
Cytogenetics 1q42.12
Domains P5CR
Protein Pathways Arginine and proline metabolism, Metabolic pathways
Summary This gene belongs to the pyrroline-5-carboxylate reductase family. The encoded mitochondrial protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. Alternatively spliced transcript variants have been described for this gene. provided by RefSeq, Nov 2012
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"NM_013328" in other vectors (7)
SKU Description Size Price
RC204525 PYCR2 (Myc-DDK-tagged)-Human pyrroline-5-carboxylate reductase family, member 2 (PYCR2) 10 ug
$300.00
RC204525L1 Lenti ORF clone of Human pyrroline-5-carboxylate reductase family, member 2 (PYCR2), Myc-DDK-tagged 10 ug
$600.00
RC204525L2 Lenti ORF clone of Human pyrroline-5-carboxylate reductase family, member 2 (PYCR2), mGFP tagged 10 ug
$600.00
RC204525L3 Lenti ORF clone of Human pyrroline-5-carboxylate reductase family, member 2 (PYCR2), Myc-DDK-tagged 10 ug
$600.00
RC204525L4 Lenti ORF clone of Human pyrroline-5-carboxylate reductase family, member 2 (PYCR2), mGFP tagged 10 ug
$600.00
SC115284 PYCR2 (untagged)-Human pyrroline-5-carboxylate reductase family, member 2 (PYCR2) 10 ug
$300.00
SC322308 PYCR2 (untagged)-Human pyrroline-5-carboxylate reductase family, member 2 (PYCR2) 10 ug
$300.00

Plasmid Map for RG204525

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG204525
Circular map for RG204525

file-alt Citations

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