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TFAP4 (NM_003223) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG203773
TFAP4 (tGFP-tagged) - Human transcription factor AP-4 (activating enhancer binding protein 4) (TFAP4)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $657.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TFAP4
Synonyms AP-4; bHLHc41
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG203773 representing NM_003223
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTATTTCATGGTGCCCACTCAGAAGGTGCCCTCTTTGCAACATTTCAGGAAAACAGAGAAAGAAG
TGATAGGAGGGCTCTGTAGCCTTGCCAACATTCCACTAACCCCCGAGACTCAGCGGGACCAGGAGCGGCG
GATTCGGCGGGAGATCGCCAACAGCAACGAGCGGAGACGCATGCAGAGCATCAACGCGGGATTCCAGTCC
CTCAAGACCCTCATCCCCCACACAGACGGAGAGAAGCTCAGCAAGGCAGCCATTCTCCAGCAGACAGCCG
AGTACATCTTCTCCCTGGAGCAGGAGAAGACCAGGCTCTTGCAGCAGAACACACAGCTCAAGCGCTTCAT
CCAGGAGCTGAGCGGCTCGTCCCCCAAGCGACGGCGGGCAGAGGACAAGGACGAAGGCATAGGCTCCCCG
GACATCTGGGAGGACGAGAAGGCGGAGGACCTGCGGCGGGAGATGATTGAGCTGCGGCAGCAGCTGGACA
AGGAGCGCTCGGTGCGCATGATGCTGGAGGAGCAGGTGCGCTCGCTGGAGGCCCACATGTACCCGGAAAA
GCTCAAGGTGATTGCGCAGCAGGTGCAGCTGCAGCAGCAGCAGGAACAGGTGAGGCTGCTGCACCAGGAG
AAGCTGGAGCGGGAACAGCAGCAGCTGCGGACCCAGCTTCTGCCCCCTCCGGCCCCCACCCACCACCCCA
CGGTGATCGTGCCAGCACCGCCTCCTCCTCCCTCCCACCACATCAATGTCGTCACCATGGGCCCCTCCTC
GGTCATCAACTCTGTTTCCACATCCCGGCAAAATCTGGACACCATCGTGCAGGCAATCCAGCACATCGAG
GGCACCCAGGAAAAGCAGGAGCTGGAGGAGGAGCAGCGGCGAGCTGTCATCGTGAAGCCTGTCCGCAGCT
GCCCGGAGGCCCCCACCTCTGACACCGCCTCCGACTCCGAGGCCTCAGACAGTGACGCCATGGACCAGAG
CCGGGAGGAGCCGTCGGGGGACGGGGAGCTTCCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG203773 representing NM_003223
Red=Cloning site Green=Tags(s)

MEYFMVPTQKVPSLQHFRKTEKEVIGGLCSLANIPLTPETQRDQERRIRREIANSNERRRMQSINAGFQS
LKTLIPHTDGEKLSKAAILQQTAEYIFSLEQEKTRLLQQNTQLKRFIQELSGSSPKRRRAEDKDEGIGSP
DIWEDEKAEDLRREMIELRQQLDKERSVRMMLEEQVRSLEAHMYPEKLKVIAQQVQLQQQQEQVRLLHQE
KLEREQQQLRTQLLPPPAPTHHPTVIVPAPPPPPSHHINVVTMGPSSVINSVSTSRQNLDTIVQAIQHIE
GTQEKQELEEEQRRAVIVKPVRSCPEAPTSDTASDSEASDSDAMDQSREEPSGDGELP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003223
ORF Size 1014 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003223.3
RefSeq Size 2149 bp
RefSeq ORF 1017 bp
Locus ID 7023
UniProt ID Q01664
Cytogenetics 16p13.3
Protein Families Druggable Genome, Transcription Factors
Summary Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG (Mermod et al., 1988 PubMed 2833704; Hu et al., 1990 PubMed 2123466).supplied by OMIM, Jul 2009
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"NM_003223" in other vectors (6)
SKU Description Size Price
RC203773 TFAP4 (Myc-DDK-tagged)-Human transcription factor AP-4 (activating enhancer binding protein 4) (TFAP4) 10 ug
$457.00
RC203773L1 Lenti ORF clone of Human transcription factor AP-4 (activating enhancer binding protein 4) (TFAP4), Myc-DDK-tagged 10 ug
$757.00
RC203773L2 Lenti ORF clone of Human transcription factor AP-4 (activating enhancer binding protein 4) (TFAP4), mGFP tagged 10 ug
$757.00
RC203773L3 Lenti ORF clone of Human transcription factor AP-4 (activating enhancer binding protein 4) (TFAP4), Myc-DDK-tagged 10 ug
$757.00
RC203773L4 Lenti ORF clone of Human transcription factor AP-4 (activating enhancer binding protein 4) (TFAP4), mGFP tagged 10 ug
$757.00
SC122647 TFAP4 (untagged)-Human transcription factor AP-4 (activating enhancer binding protein 4) (TFAP4) 10 ug
$457.00

Plasmid Map for RG203773

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG203773
Circular map for RG203773

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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