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VASP (NM_003370) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG203544
VASP (tGFP-tagged) - Human vasodilator-stimulated phosphoprotein (VASP)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
$489.00 $886.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol VASP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003370
ORF Size 1140 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003370.3, NP_003361.1
RefSeq Size 2298 bp
RefSeq ORF 1143 bp
Locus ID 7408
UniProt ID P50552
Cytogenetics 19q13.32
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways Fc gamma R-mediated phagocytosis, Focal adhesion, Leukocyte transendothelial migration
Summary Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. provided by RefSeq, Jul 2008
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"NM_003370" in other vectors (6)
SKU Description Size Price
RC203544 VASP (Myc-DDK-tagged)-Human vasodilator-stimulated phosphoprotein (VASP) 10 ug
$686.00
RC203544L1 Lenti-ORF clone of VASP (Myc-DDK-tagged)-Human vasodilator-stimulated phosphoprotein (VASP) 10 ug
$986.00
RC203544L2 Lenti-ORF clone of VASP (mGFP-tagged)-Human vasodilator-stimulated phosphoprotein (VASP) 10 ug
$986.00
RC203544L3 Lenti-ORF clone of VASP (Myc-DDK-tagged)-Human vasodilator-stimulated phosphoprotein (VASP) 10 ug
$986.00
RC203544L4 Lenti-ORF clone of VASP (mGFP-tagged)-Human vasodilator-stimulated phosphoprotein (VASP) 10 ug
$986.00
SC122656 VASP (untagged)-Human vasodilator-stimulated phosphoprotein (VASP) 10 ug
$686.00

Plasmid Map for RG203544

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Cloning scheme for RG203544
Circular map for RG203544

file-alt Citations

Vasodilator-Stimulated Phosphoprotein Activity Is Required for Coxiella burnetii Growth in Human Macrophages
Colonne, Winchell, Graham et al
PLoS Pathog. (2016) 12 (10), e1005915 DOI: 10.1371/journal.ppat.1005915
AI Summary: OriGene's pCMV6-AC-GFP vectors containing full-length VASP and the S239E mutant cDNA were used to express GFP-tagged VASP proteins, enabling the study of VASP's role in Coxiella burnetii infection of human macrophages.
Snippet: Expression of GFP-VASP and GFP-VASP (S239E) was achieved using full length VASP or S239E mutant cDNA cloned into pCMV6-AC-GFP (OriGene)
FigureFigureFigure
Showing 1-1 of 1 papers.
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