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RPP25 (NM_017793) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG203538
RPP25 (tGFP-tagged) - Human ribonuclease P/MRP 25kDa subunit (RPP25)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "RPP25 or NM_017793"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RPP25
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG203538 representing NM_017793
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGGCGCGCCC

ATGGAGAACTTCCGTAAGGTGCGCTCCGAAGAGGCGCCAGCGGGGTGCGGGGCCGAGGGAGGCGGCCCGG
GCTCCGGCCCCTTCGCAGACCTGGCGCCGGGCGCGGTGCACATGCGGGTCAAGGAAGGCAGCAAGATCCG
GAACCTGATGGCCTTCGCCACCGCCAGCATGGCGCAGCCAGCCACGCGCGCCATCGTCTTCAGCGGCTGC
GGCCGGGCCACCACCAAAACCGTCACGTGCGCCGAGATCCTCAAGCGCCGCCTGGCGGGCCTGCACCAGG
TCACGCGGCTGCGCTACCGGAGCGTACGCGAGGTGTGGCAGAGCCTCCCGCCTGGGCCCACGCAGGGTCA
GACGCCTGGCGAGCCGGCCGCTAGTCTCAGCGTACTTAAGAACGTGCCCGGCCTCGCCATCCTACTTTCC
AAGGACGCGCTGGATCCGCGACAGCCCGGCTACCAGCCCCCGAATCCCCATCCTGGTCCCTCGTCCCCGC
CAGCCGCGCCAGCGTCCAAGAGGAGCCTAGGGGAACCCGCAGCTGGAGAAGGCTCCGCGAAGCGATCGCA
ACCCGAGCCAGGGGTTGCGGACGAGGATCAGACGGCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG203538 representing NM_017793
Red=Cloning site Green=Tags(s)

MENFRKVRSEEAPAGCGAEGGGPGSGPFADLAPGAVHMRVKEGSKIRNLMAFATASMAQPATRAIVFSGC
GRATTKTVTCAEILKRRLAGLHQVTRLRYRSVREVWQSLPPGPTQGQTPGEPAASLSVLKNVPGLAILLS
KDALDPRQPGYQPPNPHPGPSSPPAAPASKRSLGEPAAGEGSAKRSQPEPGVADEDQTA

TRTRPLE - GFP Tag - V
Restriction Sites AscI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017793
ORF Size 597 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017793.3
RefSeq Size 2333 bp
RefSeq ORF 600 bp
Locus ID 54913
UniProt ID Q9BUL9
Cytogenetics 15q24.2
Summary Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends (PubMed:12003489, PubMed:16723659, PubMed:30454648). Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences (PubMed:28115465).UniProtKB/Swiss-Prot Function
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"NM_017793" in other vectors (4)
SKU Description Size Price
RC203538 RPP25 (Myc-DDK-tagged)-Human ribonuclease P/MRP 25kDa subunit (RPP25) 10 ug
$300.00
RC203538L3 Lenti ORF clone of Human ribonuclease P/MRP 25kDa subunit (RPP25), Myc-DDK-tagged 10 ug
$600.00
RC203538L4 Lenti ORF clone of Human ribonuclease P/MRP 25kDa subunit (RPP25), mGFP tagged 10 ug
$600.00
SC320291 RPP25 (untagged)-Human ribonuclease P/MRP 25kDa subunit (RPP25) 10 ug
$300.00

Plasmid Map for RG203538

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG203538
Circular map for RG203538

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