SAT1 (NM_002970) Human Tagged ORF Clone
- TrueORF®
SKU
RG203275
SAT1 (tGFP-tagged) - Human spermidine/spermine N1-acetyltransferase 1 (SAT1), transcript variant 1
$489.00
$650.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | SAT1 |
| Synonyms | DC21; KFSD; KFSDX; SAT; SSAT; SSAT-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_002970 |
| ORF Size | 513 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002970.3 |
| RefSeq Size | 1060 bp |
| RefSeq ORF | 516 bp |
| Locus ID | 6303 |
| UniProt ID | P21673 |
| Cytogenetics | Xp22.11 |
| Domains | Acetyltransf |
| Protein Families | Druggable Genome |
| Protein Pathways | Arginine and proline metabolism, Metabolic pathways |
| Summary | The protein encoded by this gene belongs to the acetyltransferase family, and is a rate-limiting enzyme in the catabolic pathway of polyamine metabolism. It catalyzes the acetylation of spermidine and spermine, and is involved in the regulation of the intracellular concentration of polyamines and their transport out of cells. Defects in this gene are associated with keratosis follicularis spinulosa decalvans (KFSD). Alternatively spliced transcripts have been found for this gene.provided by RefSeq, Sep 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002970" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC203275 | SAT1 (Myc-DDK-tagged)-Human spermidine/spermine N1-acetyltransferase 1 (SAT1), transcript variant 1 | 10 ug |
$450.00
|
|
| RC203275L1 | Lenti ORF clone of Human spermidine/spermine N1-acetyltransferase 1 (SAT1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC203275L2 | Lenti ORF clone of Human spermidine/spermine N1-acetyltransferase 1 (SAT1), transcript variant 1, mGFP tagged | 10 ug |
$750.00
|
|
| RC203275L3 | Lenti ORF clone of Human spermidine/spermine N1-acetyltransferase 1 (SAT1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC203275L4 | Lenti ORF clone of Human spermidine/spermine N1-acetyltransferase 1 (SAT1), transcript variant 1, mGFP tagged | 10 ug |
$750.00
|
|
| SC118307 | SAT1 (untagged)-Human spermidine/spermine N1-acetyltransferase 1 (SAT1), transcript variant 1 | 10 ug |
$450.00
|
|
| SC320861 | SAT1 (untagged)-Human spermidine/spermine N1-acetyltransferase 1 (SAT1), transcript variant 1 | 10 ug |
$450.00
|
|
Citations
Promotion of homology-directed DNA repair by polyamines
Lee, Su, Huang et al
Nat Commun (2019) 10 (1), 65 DOI: 10.1038/s41467-018-08011-1
Lee, Su, Huang et al
Nat Commun (2019) 10 (1), 65 DOI: 10.1038/s41467-018-08011-1
AI Summary: OriGene products contributed to this study by providing the human SAT1 expression plasmid and the pCMV6-AC-GFP vector used for cloning human ODC1 cDNA, enabling the investigation of polyamine-related gene functions in DNA repair.
Snippet: 2′-deoxyuridine (BrdU), and N-(2-Aminoethyl)−1,3-propanediamine were purchased from Sigma. Plasmids The human ODC1 cDNA (RefSeq NM_002539.2) was cloned into NotI and PmeI sites of pCMV6-AC-GFP (OriGene). The human SAT1 (RefSeq NM_002970.1) expression plasmid was purchased from OriGene. The human RAD51 cDNA (RefSeq NM_002875.4) was cloned into the XbaI and PvuII sites of pCBASceI to replace t
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