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TSSC4 (NM_005706) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG202870
TSSC4 (tGFP-tagged) - Human tumor suppressing subtransferable candidate 4 (TSSC4)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TSSC4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG202870 representing NM_005706
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGAGGCAGGAACAGGTGAGCCGTCCCCCAGCGTGGAGGGCGAACACGGGACGGAGTATGACACGC
TGCCTTCCGACACAGTCTCCCTCAGTGACTCGGACTCTGACCTCAGCTTGCCCGGTGGTGCTGAAGTGGA
AGCACTGTCCCCGATGGGGCTGCCTGGGGAGGAGGATTCAGGTCCTGATGAGCCGCCCTCACCCCCGTCA
GGCCTCCTCCCAGCCACGGTGCAGCCATTCCATCTGAGAGGCATGAGCTCCACCTTCTCCCAGCGCAGCC
GTGACATCTTTGACTGCCTGGAGGGGGCGGCCAGACGGGCTCCATCCTCTGTGGCCCACACCAGCATGAG
TGACAACGGAGGCTTCAAGCGGCCCCTAGCGCCCTCAGGCCGGTCTCCAGTGGAAGGCCTGGGCAGGGCC
CATCGGAGCCCTGCCTCACCAAGGGTGCCTCCGGTCCCCGACTACGTGGCACACCCCGAGCGCTGGACCA
AGTACAGCCTGGAAGATGTGACCGAGGTCAGCGAGCAGAGCAATCAGGCCACCGCCCTGGCCTTCCTGGG
CTCCCAGAGCCTGGCTGCCCCCACTGACTGCGTGTCCTCCTTCAACCAGGATCCCTCCAGCTGTGGGGAG
GGGAGGGTCATCTTCACCAAACCAGTCCGAGGGGTCGAAGCCAGACACGAGAGGAAGAGGGTCCTGGGGA
AGGTGGGAGAGCCAGGCAGGGGCGGCCTTGGGAATCCTGCCACAGACAGGGGCGAGGGCCCTGTGGAGCT
GGCCCATCTGGCCGGGCCCGGGAGCCCAGAGGCTGAGGAGTGGGGCAGCCCCCATGGAGGCCTGCAGGAG
GTGGAGGCACTGTCAGGGTCTGTCCACAGTGGGTCTGTGCCAGGTCTCCCGCCGGTGGAAACTGTTGGCT
TCCATGGCAGCAGGAAGCGGAGTCGAGACCACTTCCGGAACAAGAGCAGCAGCCCCGAGGACCCAGGTGC
TGAGGTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG202870 representing NM_005706
Red=Cloning site Green=Tags(s)

MAEAGTGEPSPSVEGEHGTEYDTLPSDTVSLSDSDSDLSLPGGAEVEALSPMGLPGEEDSGPDEPPSPPS
GLLPATVQPFHLRGMSSTFSQRSRDIFDCLEGAARRAPSSVAHTSMSDNGGFKRPLAPSGRSPVEGLGRA
HRSPASPRVPPVPDYVAHPERWTKYSLEDVTEVSEQSNQATALAFLGSQSLAAPTDCVSSFNQDPSSCGE
GRVIFTKPVRGVEARHERKRVLGKVGEPGRGGLGNPATDRGEGPVELAHLAGPGSPEAEEWGSPHGGLQE
VEALSGSVHSGSVPGLPPVETVGFHGSRKRSRDHFRNKSSSPEDPGAEV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005706
ORF Size 987 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005706.2, NP_005697.2
RefSeq Size 1443 bp
RefSeq ORF 990 bp
Locus ID 10078
UniProt ID Q9Y5U2
Cytogenetics 11p15.5
Protein Families Druggable Genome
Summary This gene is one of several tumor-suppressing subtransferable fragments located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. This gene is located among several imprinted genes; however, this gene, as well as the pan-hematopoietic expression gene (PHEMX), escapes imprinting. This gene may play a role in malignancies and disease that involve this region. Several transcript variants encoding two different isoforms have been found for this gene. provided by RefSeq, Jul 2014
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"NM_005706" in other vectors (6)
SKU Description Size Price
RC202870 TSSC4 (Myc-DDK-tagged)-Human tumor suppressing subtransferable candidate 4 (TSSC4) 10 ug
$300.00
RC202870L1 Lenti ORF clone of Human tumor suppressing subtransferable candidate 4 (TSSC4), Myc-DDK-tagged 10 ug
$600.00
RC202870L2 Lenti ORF clone of Human tumor suppressing subtransferable candidate 4 (TSSC4), mGFP tagged 10 ug
$600.00
RC202870L3 Lenti ORF clone of Human tumor suppressing subtransferable candidate 4 (TSSC4), Myc-DDK-tagged 10 ug
$600.00
RC202870L4 Lenti ORF clone of Human tumor suppressing subtransferable candidate 4 (TSSC4), mGFP tagged 10 ug
$600.00
SC319137 TSSC4 (untagged)-Human tumor suppressing subtransferable candidate 4 (TSSC4) 10 ug
$300.00

Plasmid Map for RG202870

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG202870
Circular map for RG202870

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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