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EIF1AY (NM_004681) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG202695
EIF1AY (tGFP-tagged) - Human eukaryotic translation initiation factor 1A, Y-linked (EIF1AY)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $489.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EIF1AY
Synonyms eIF-4C
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG202695 representing NM_004681
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCAAGAATAAAGGTAAAGGAGGTAAAAACAGGCGCAGGGGTAAAAATGAGAATGAATCTGAAAAAA
GAGAGTTGGTGTTTAAAGAGGATGGACAAGAGTATGCTCAGGTAATCAAAATGTTGGGAAATGGACGATT
GGAAGCATTGTGTTTTGATGGTGTAAAGAGGTTATGCCATATCAGAGGGAAATTGAGAAAAAAGGTTTGG
ATAAATACATCAGACATTATATTGGTTGGTCTACGGGACTATCAGGATAACAAAGCTGATGTAATTTTAA
AGTACAATGCAGATGAAGCTAGAAGCCTGAAGGCATATGGCGAGCTTCCAGAACATGCTAAAATCAATGA
AACAGACACATTTGGTCCTGGAGATGATGATGAAATCCAGTTTGACGATATTGGAGATGATGATGAAGAC
ATTGATGATATC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG202695 representing NM_004681
Red=Cloning site Green=Tags(s)

MPKNKGKGGKNRRRGKNENESEKRELVFKEDGQEYAQVIKMLGNGRLEALCFDGVKRLCHIRGKLRKKVW
INTSDIILVGLRDYQDNKADVILKYNADEARSLKAYGELPEHAKINETDTFGPGDDDEIQFDDIGDDDED
IDDI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004681
ORF Size 432 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004681.4
RefSeq Size 1399 bp
RefSeq ORF 435 bp
Locus ID 9086
UniProt ID O14602
Cytogenetics Yq11.223
Domains eIF-1a
Summary This gene is located on the non-recombining region of the Y chromosome. It encodes a protein related to eukaryotic translation initiation factor 1A (EIF1A), which may function in stabilizing the binding of the initiator Met-tRNA to 40S ribosomal subunits. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2013
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"NM_004681" in other vectors (4)
SKU Description Size Price
RC202695 EIF1AY (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 1A, Y-linked (EIF1AY) 10 ug
$150.00
RC202695L3 Lenti ORF clone of Human eukaryotic translation initiation factor 1A, Y-linked (EIF1AY), Myc-DDK-tagged 10 ug
$450.00
RC202695L4 Lenti ORF clone of Human eukaryotic translation initiation factor 1A, Y-linked (EIF1AY), mGFP tagged 10 ug
$450.00
SC127766 EIF1AY (untagged)-Human eukaryotic translation initiation factor 1A, Y-linked (EIF1AY) 10 ug
$150.00

Plasmid Map for RG202695

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG202695
Circular map for RG202695

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