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EIF1B (NM_005875) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG202691
EIF1B (tGFP-tagged) - Human eukaryotic translation initiation factor 1B (EIF1B)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $350.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EIF1B
Synonyms GC20
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG202691 representing NM_005875
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCACTATCCAGAACCTCCAATCTTTCGACCCCTTTGCTGATGCAACTAAGGGTGACGACTTACTCC
CGGCAGGGACTGAGGATTACATTCATATAAGAATCCAGCAACGGAACGGCAGAAAGACACTGACTACTGT
TCAGGGCATTGCAGATGATTATGACAAAAAGAAACTTGTGAAAGCTTTCAAAAAGAAATTTGCCTGTAAT
GGTACTGTGATTGAACATCCTGAATACGGAGAGGTTATTCAGCTTCAAGGTGACCAAAGAAAAAACATCT
GCCAGTTTCTCTTGGAGGTTGGCATTGTAAAGGAGGAACAGCTTAAGGTTCATGGATTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG202691 representing NM_005875
Red=Cloning site Green=Tags(s)

MSTIQNLQSFDPFADATKGDDLLPAGTEDYIHIRIQQRNGRKTLTTVQGIADDYDKKKLVKAFKKKFACN
GTVIEHPEYGEVIQLQGDQRKNICQFLLEVGIVKEEQLKVHGF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005875
ORF Size 339 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005875.3
RefSeq Size 1014 bp
RefSeq ORF 342 bp
Locus ID 10289
UniProt ID O60739
Cytogenetics 3p22.1
Domains SUI1
Summary Probably involved in translation.UniProtKB/Swiss-Prot Function
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"NM_005875" in other vectors (4)
SKU Description Size Price
RC202691 EIF1B (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 1B (EIF1B) 10 ug
$150.00
RC202691L3 Lenti ORF clone of Human eukaryotic translation initiation factor 1B (EIF1B), Myc-DDK-tagged 10 ug
$450.00
RC202691L4 Lenti ORF clone of Human eukaryotic translation initiation factor 1B (EIF1B), mGFP tagged 10 ug
$450.00
SC111145 EIF1B (untagged)-Human eukaryotic translation initiation factor 1B (EIF1B) 10 ug
$150.00

Plasmid Map for RG202691

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file-download TXT (FASTA format)

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Cloning scheme for RG202691
Circular map for RG202691

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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