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NRL (NM_006177) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG202237
NRL (tGFP-tagged) - Human neural retina leucine zipper (NRL)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $500.00
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Other products for "NRL or NM_006177"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NRL
Synonyms D14S46E; NRL-MAF; RP27
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006177
ORF Size 711 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006177.5
RefSeq Size 2102 bp
RefSeq ORF 714 bp
Locus ID 4901
UniProt ID P54845
Cytogenetics 14q11.2-q12
Protein Families Druggable Genome, Transcription Factors
Summary This gene encodes a basic motif-leucine zipper transcription factor of the Maf subfamily. The encoded protein is conserved among vertebrates and is a critical intrinsic regulator of photoceptor development and function. Mutations in this gene have been associated with retinitis pigmentosa and retinal degenerative diseases. provided by RefSeq, Jul 2008
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"NM_006177" in other vectors (6)
SKU Description Size Price
RC202237 NRL (Myc-DDK-tagged)-Human neural retina leucine zipper (NRL) 10 ug
$300.00
RC202237L1 Lenti ORF clone of Human neural retina leucine zipper (NRL), Myc-DDK-tagged 10 ug
$600.00
RC202237L2 Lenti ORF clone of Human neural retina leucine zipper (NRL), mGFP tagged 10 ug
$600.00
RC202237L3 Lenti ORF clone of Human neural retina leucine zipper (NRL), Myc-DDK-tagged 10 ug
$600.00
RC202237L4 Lenti ORF clone of Human neural retina leucine zipper (NRL), mGFP tagged 10 ug
$600.00
SC125982 NRL (untagged)-Human neural retina leucine zipper (NRL) 10 ug
$300.00

Plasmid Map for RG202237

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file-download TXT (FASTA format)

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Cloning scheme for RG202237
Circular map for RG202237

file-alt Citations

In vivo efficacy of NRL knockdown with cell-penetrating siRNA in retinal degeneration
Lee, Jang, Chae et al
Sci Rep (2025) 15 (1), 27176 DOI: 10.1038/s41598-025-07299-6
Cited by 2
AI Summary: OriGene’s NRL plasmid (RG202237) was used to transfect cells and generate NRL-expressing cell models, enabling the study to assess the efficacy of NRL knockdown by cp-asiNRL in vitro.
Snippet: re cultured in DMEM (Invitrogen) supplemented with 10% FBS, 100 U/ml penicillin, and 100 µg/ml streptomycin at 30–50% confluence. After 24 h, cells were transfected with 0.5 µg of NRL plasmid (Origene, RG202237) using Lipofectamine 2000 (Invitrogen) in Opti-MEM I Reduced Serum Medium (Gibco). Cells were harvested after 24–48 h for analysis. A549 cells stably expressing NRL were cultur
FigureFigureFigure
Showing 1-1 of 1 papers.
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