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MAP1 (MOAP1) (NM_022151) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG202203
MOAP1 (tGFP-tagged) - Human modulator of apoptosis 1 (MOAP1)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $657.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MAP1
Synonyms MAP-1; PNMA4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG202203 representing NM_022151
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTTTGAGGCTTTTAGAAGACTGGTGCAGGGGGATGGACATGAACCCTCGGAAAGCGCTATTGATTG
CCGGCATCTCCCAGAGCTGCAGTGTGGCAGAAATCGAGGAGGCTCTGCAGGCTGGTTTAGCTCCCTTGGG
GGAGTACAGACTGCTTGGAAGGATGTTCAGGAGGGATGAGAACAGGAAAGTAGCCTTAGTAGGGCTTACT
GCGGAGACTAGTCACGCCCTGGTCCCTAAGGAGATACCGGGAAAAGGGGGTATCTGGAGAGTGATCTTTA
AGCCCCCTGACCCAGATAATACATTTTTAAGCAGATTAAATGAATTTTTAGCGGGAGAGGGCATGACAGT
GGGTGAGTTGAGCAGAGCTCTTGGACATGAAAATGGCTCCTTAGACCCAGAGCAGGGCATGATCCCGGAA
ATGTGGGCCCCTATGTTGGCACAGGCATTAGAGGCTCTTCAGCCTGCCCTGCAATGCTTGAAGTATAAAA
AGCTGAGAGTGTTCTCGGGCAGGGAGTCTCCAGAACCAGGAGAAGAAGAATTTGGACGCTGGATGTTTCA
TACTACTCAGATGATAAAGGCGTGGCAGGTGCCAGATGTAGAGAAGAGAAGGCGATTGCTAGAGAGCCTT
CGAGGCCCAGCACTTGATGTTATTCGTGTCCTCAAGATAAACAATCCTTTAATTACTGTCGATGAATGTC
TGCAGGCTCTTGAGGAGGTATTTGGGGTTACAGATAATCCTAGGGAGTTGCAGGTCAAATATCTAACCAC
TTACCAGAAGGATGAGGAAAAGTTGTCGGCTTATGTACTAAGGCTGGAGCCTTTGTTACAGAAGCTGGTA
CAGAGAGGAGCAATTGAGAGAGATGCTGTGAATCAGGCCCGCCTAGACCAAGTCATTGCTGGGGCAGTCC
ACAAAACAATTCGCAGAGAGCTTAATCTGCCAGAGGATGGCCCAGCCCCTGGTTTCTTGCAGTTATTGGT
ACTAATAAAGGATTATGAGGCAGCTGAGGAGGAGGAGGCCCTTCTCCAGGCAATATTGGAAGGTAATTTC
ACC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG202203 representing NM_022151
Red=Cloning site Green=Tags(s)

MTLRLLEDWCRGMDMNPRKALLIAGISQSCSVAEIEEALQAGLAPLGEYRLLGRMFRRDENRKVALVGLT
AETSHALVPKEIPGKGGIWRVIFKPPDPDNTFLSRLNEFLAGEGMTVGELSRALGHENGSLDPEQGMIPE
MWAPMLAQALEALQPALQCLKYKKLRVFSGRESPEPGEEEFGRWMFHTTQMIKAWQVPDVEKRRRLLESL
RGPALDVIRVLKINNPLITVDECLQALEEVFGVTDNPRELQVKYLTTYQKDEEKLSAYVLRLEPLLQKLV
QRGAIERDAVNQARLDQVIAGAVHKTIRRELNLPEDGPAPGFLQLLVLIKDYEAAEEEEALLQAILEGNF
T

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022151
ORF Size 1053 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022151.5
RefSeq Size 2435 bp
RefSeq ORF 1056 bp
Locus ID 64112
UniProt ID Q96BY2
Cytogenetics 14q32.12
Summary The protein encoded by this gene was identified by its interaction with apoptosis regulator BAX protein. This protein contains a Bcl-2 homology 3 (BH3)-like motif, which is required for the association with BAX. When overexpressed, this gene has been shown to mediate caspase-dependent apoptosis. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_022151" in other vectors (5)
SKU Description Size Price
RC202203 MOAP1 (Myc-DDK-tagged)-Human modulator of apoptosis 1 (MOAP1) 10 ug
$457.00
RC202203L3 Lenti ORF clone of Human modulator of apoptosis 1 (MOAP1), Myc-DDK-tagged 10 ug
$757.00
RC202203L4 Lenti ORF clone of Human modulator of apoptosis 1 (MOAP1), mGFP tagged 10 ug
$757.00
SC112815 MOAP1 (untagged)-Human modulator of apoptosis 1 (MOAP1) 10 ug
$457.00
SC322373 MOAP1 (untagged)-Human modulator of apoptosis 1 (MOAP1) 10 ug
$457.00

Plasmid Map for RG202203

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG202203
Circular map for RG202203

file-alt Citations

miR-25 targets the modulator of apoptosis 1 gene in lung cancer
Wu, Chen, Kong et al
Carcinogenesis (2015)
Cited by 76
AI Summary: OriGene's pCMV6-AC–GFP–MOAP1 vector was used in this study to overexpress the MOAP1 gene, enabling the authors to investigate how miR-25 regulates MOAP1 and affects lung cancer cell proliferation and apoptosis.
Snippet: 3 cells per well in 96-well plates transfected with 200 ng pSIF–GFP–miR-25 and/or 20 ng pCMV6-AC–GFP–MOAP1 vector (Origene, Rockville, MD, USA). For flow cytometry analyses and Western blotting, cells were seeded at 2×10 5 cells per well in ... Cite SaveSaving
Showing 1-1 of 1 papers.
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