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AKAP10 (NM_007202) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG202174
AKAP10 (tGFP-tagged) - Human A kinase (PRKA) anchor protein 10 (AKAP10), nuclear gene encoding mitochondrial protein
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
$489.00 $816.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "AKAP10 or NM_007202"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol AKAP10
Synonyms AKAP-10; D-AKAP-2; D-AKAP2; PRKA10
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG202174 representing NM_007202
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGGGAGCCGGGCCCTCCCCGCGCCAGTCCCCCCGCACCCTCCGTCCCGACCCGGGCCCCGCCATGT
CCTTCTTCCGGCGGAAAGTGAAAGGCAAAGAACAAGAGAAGACCTCAGATGTGAAGTCCATTAAAGCTTC
AATATCCGTACATTCCCCACAAAAAAGCACTAAAAATCATGCCTTGCTGGAGGCTGCAGGACCAAGTCAT
GTTGCAATCAATGCCATTTCTGCCAACATGGACTCCTTTTCAAGTAGCAGGACAGCCACACTTAAGAAGC
AGCCAAGCCACATGGAGGCCGCTCATTTTGGTGACCTGGGCAGATCTTGTCTGGACTACCAGACTCAAGA
GACCAAATCAAGCCTTTCTAAGACCCTTGAACAAGTCTTGCACGACACTATTGTCCTCCCTTACTTCATT
CAATTCATGGAACTTCGGCGAATGGAGCATTTGGTGAAATTTTGGTTAGAGGCTGAAAGTTTTCATTCAA
CAACTTGGTCGCGAATAAGAGCACACAGTCTAAACACAGTGAAGCAGAGCTCACTGGCTGAGCCTGTCTC
TCCATCTAAAAAGCATGAAACTACAGCGTCTTTTTTAACTGATTCTCTTGATAAGAGATTGGAGGATTCT
GGCTCAGCACAGTTGTTTATGACTCATTCAGAAGGAATTGACCTGAATAATAGAACTAACAGCACTCAGA
ATCACTTGCTGCTTTCCCAGGAATGTGACAGTGCCCATTCTCTCCGTCTTGAAATGGCCAGAGCAGGAAC
TCACCAAGTTTCCATGGAAACCCAAGAATCTTCCTCTACACTTACAGTAGCCAGTAGAAATAGTCCCGCT
TCTCCACTAAAAGAATTGTCAGGAAAACTAATGAAAAGTATAGAACAAGATGCAGTGAATACTTTTACCA
AATATATATCTCCAGATGCTGCTAAACCAATACCAATTACAGAAGCAATGAGAAATGACATCATAGCAAG
GATTTGTGGAGAAGATGGACAGGTGGATCCCAACTGTTTCGTTTTGGCACAGTCCATAGTCTTTAGTGCA
ATGGAGCAAGAGCACTTTAGTGAGTTTCTGCGAAGTCACCATTTCTGTAAATACCAGATTGAAGTGCTGA
CCAGTGGAACTGTTTACCTGGCTGACATTCTCTTCTGTGAGTCAGCCCTCTTTTATTTCTCTGAGTACAT
GGAAAAAGAGGATGCAGTGAATATCTTACAATTCTGGTTGGCAGCAGATAACTTCCAGTCTCAGCTTGCT
GCCAAAAAGGGCCAATATGATGGACAGGAGGCACAGAATGATGCCATGATTTTATATGACAAGTACTTCT
CCCTCCAAGCCACACATCCTCTTGGATTTGATGATGTTGTACGATTAGAAATTGAATCCAATATCTGCAG
GGAAGGTGGGCCACTCCCCAACTGTTTCACAACTCCATTACGTCAGGCCTGGACAACCATGGAGAAGGTC
TTTTTGCCTGGCTTTTTGTCCAGCAATCTTTATTATAAATATTTGAATGATCTCATCCATTCGGTTCGAG
GAGATGAATTTCTGGGCGGGAACGTGTCGCTGACTGCTCCTGGCTCTGTTGGCCCTCCTGATGAGTCTCA
CCCAGGGAGTTCTGACAGCTCTGCGTCTCAGTCCAGTGTGAAAAAAGCCAGTATTAAAATACTGAAAAAT
TTTGATGAAGCGATAATTGTGGATGCGGCAAGTCTGGATCCAGAATCTTTATATCAACGGACATATGCCG
GGAAGATGACATTTGGAAGAGTCAGTGACTTGGGGCAATTCATCCGAGAATCTGAGCCTGAACCTGATGT
AAGGAAATCAAAAGGATCCATGTTCTCACAAGCTATGAAGAAATGGGTGCAAGGAAATACTGATGAGGCC
CAGGAAGAGCTAGCTTGGAAGATTGCTAAAATGATAGTCAGTGACATTATGCAGCAGGCTCAGTATGATC
AACCGTTAGAGAAATCTACAAAGTTA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG202174 representing NM_007202
Red=Cloning site Green=Tags(s)

MRGAGPSPRQSPRTLRPDPGPAMSFFRRKVKGKEQEKTSDVKSIKASISVHSPQKSTKNHALLEAAGPSH
VAINAISANMDSFSSSRTATLKKQPSHMEAAHFGDLGRSCLDYQTQETKSSLSKTLEQVLHDTIVLPYFI
QFMELRRMEHLVKFWLEAESFHSTTWSRIRAHSLNTVKQSSLAEPVSPSKKHETTASFLTDSLDKRLEDS
GSAQLFMTHSEGIDLNNRTNSTQNHLLLSQECDSAHSLRLEMARAGTHQVSMETQESSSTLTVASRNSPA
SPLKELSGKLMKSIEQDAVNTFTKYISPDAAKPIPITEAMRNDIIARICGEDGQVDPNCFVLAQSIVFSA
MEQEHFSEFLRSHHFCKYQIEVLTSGTVYLADILFCESALFYFSEYMEKEDAVNILQFWLAADNFQSQLA
AKKGQYDGQEAQNDAMILYDKYFSLQATHPLGFDDVVRLEIESNICREGGPLPNCFTTPLRQAWTTMEKV
FLPGFLSSNLYYKYLNDLIHSVRGDEFLGGNVSLTAPGSVGPPDESHPGSSDSSASQSSVKKASIKILKN
FDEAIIVDAASLDPESLYQRTYAGKMTFGRVSDLGQFIRESEPEPDVRKSKGSMFSQAMKKWVQGNTDEA
QEELAWKIAKMIVSDIMQQAQYDQPLEKSTKL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007202
ORF Size 1986 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007202.4
RefSeq Size 2929 bp
RefSeq ORF 1989 bp
Locus ID 11216
UniProt ID O43572
Cytogenetics 17p11.2
Domains RGS
Protein Families Druggable Genome
Summary This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins bind to the regulatory subunits of protein kinase A (PKA) and confine the holoenzyme to discrete locations within the cell. The encoded protein is localized to mitochondria and interacts with both the type I and type II regulatory subunits of PKA. Polymorphisms in this gene may be associated with increased risk of arrhythmias and sudden cardiac death. provided by RefSeq, May 2012
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"NM_007202" in other vectors (7)
SKU Description Size Price
RC202174 AKAP10 (Myc-DDK-tagged)-Human A kinase (PRKA) anchor protein 10 (AKAP10), nuclear gene encoding mitochondrial protein 10 ug
$289.00 $616.00
RC202174L1 Lenti ORF clone of Human A kinase (PRKA) anchor protein 10 (AKAP10), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$916.00
RC202174L2 Lenti ORF clone of Human A kinase (PRKA) anchor protein 10 (AKAP10), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$916.00
RC202174L3 Lenti ORF clone of Human A kinase (PRKA) anchor protein 10 (AKAP10), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$916.00
RC202174L4 Lenti ORF clone of Human A kinase (PRKA) anchor protein 10 (AKAP10), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$916.00
SC115681 AKAP10 (untagged)-Human A kinase (PRKA) anchor protein 10 (AKAP10), nuclear gene encoding mitochondrial protein 10 ug
$617.00
SC324657 AKAP10 (untagged)-Human A kinase (PRKA) anchor protein 10 (AKAP10), nuclear gene encoding mitochondrial protein 10 ug
$617.00

Plasmid Map for RG202174

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG202174
Circular map for RG202174

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