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BPGM (NM_199186) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG202105
BPGM (tGFP-tagged) - Human 2,3-bisphosphoglycerate mutase (BPGM), transcript variant 2
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BPGM
Synonyms DPGM; ECYT8
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_199186
ORF Size 777 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_199186.2, NP_954655.1
RefSeq Size 2074 bp
RefSeq ORF 780 bp
Locus ID 669
UniProt ID P07738
Cytogenetics 7q33
Protein Families Druggable Genome
Protein Pathways Glycolysis / Gluconeogenesis, Metabolic pathways
Summary 2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified. provided by RefSeq, Sep 2009
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"NM_199186" in other vectors (4)
SKU Description Size Price
RC202105 BPGM (Myc-DDK-tagged)-Human 2,3-bisphosphoglycerate mutase (BPGM), transcript variant 2 10 ug
$300.00
RC202105L3 Lenti ORF clone of Human 2,3-bisphosphoglycerate mutase (BPGM), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC202105L4 Lenti ORF clone of Human 2,3-bisphosphoglycerate mutase (BPGM), transcript variant 2, mGFP tagged 10 ug
$600.00
SC307893 BPGM (untagged)-Human 2,3-bisphosphoglycerate mutase (BPGM), transcript variant 2 10 ug
$330.00

Plasmid Map for RG202105

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Cloning scheme for RG202105
Circular map for RG202105

file-alt Citations

Genetic Variation, Iron Status, and FGF23 Signaling Converge to Regulate Renal Calcium Buffering in Sickle Cell Disease
Agoro, Xie, Janeczko et al
bioRxiv (2026)
Snippet: … After 24 h, cells were transfected using Fugene (FuGENE, 4K-1000) with 1 µg of a BPGM-encoding, GFP-tagged ORF expression vector (OriGene, RG202105) or empty expression vector pCMV6 (OriGene, PS100001). …
Showing 1-1 of 1 papers.
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