alpha 1 Antitrypsin (SERPINA1) (NM_000295) Human Tagged ORF Clone
- TrueORF®
SKU
RG202082
SERPINA1 (tGFP-tagged) - Human serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 1
$489.00
$657.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | alpha 1 Antitrypsin |
| Synonyms | A1A; A1AT; AAT; alpha1AT; nNIF; PI; PI1; PRO2275 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000295 |
| ORF Size | 1254 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000295.2 |
| RefSeq Size | 1607 bp |
| RefSeq ORF | 1257 bp |
| Locus ID | 5265 |
| UniProt ID | P01009, E9KL23 |
| Cytogenetics | 14q32.13 |
| Domains | SERPIN |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein |
| Protein Pathways | Complement and coagulation cascades |
| Summary | The protein encoded by this gene is a serine protease inhibitor belonging to the serpin superfamily whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. This protein is produced in the liver, the bone marrow, by lymphocytic and monocytic cells in lymphoid tissue, and by the Paneth cells of the gut. Defects in this gene are associated with chronic obstructive pulmonary disease, emphysema, and chronic liver disease. Several transcript variants encoding the same protein have been found for this gene. provided by RefSeq, Aug 2020 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000295" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202082 | SERPINA1 (Myc-DDK-tagged)-Human serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 1 | 10 ug |
$457.00
|
|
| RC202082L1 | Lenti ORF clone of Human serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC202082L2 | Lenti ORF clone of Human serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 1, mGFP tagged | 10 ug |
$757.00
|
|
| RC202082L3 | Lenti ORF clone of Human serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC202082L4 | Lenti ORF clone of Human serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 1, mGFP tagged | 10 ug |
$757.00
|
|
| SC119951 | SERPINA1 (untagged)-Human serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 1 | 10 ug |
$457.00
|
|
| SC322312 | SERPINA1 (untagged)-Human serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 1 | 10 ug |
$457.00
|
|
Citations
LIPOSOMAL TECHNOLOGIES TO IMPROVE GENE DELIVERY
Nardo Padron
Thesis (2022)
Nardo Padron
Thesis (2022)
Snippet: r 1 hour at 37 °C. The plate was then washed again, and 100 μL of fresh media from cells were plated, in duplicate, along with a standard curve made by serially diluting purified hAAT (OriGene No. RG202082) in PBS-C from 50 ng/mL to 0.048 ng/mL and incubated for 1 hour at 37 °C. The plate was then washed and 50 μL of mouse anti-hAAT monoclonal IgG2a a
Cyanuric chloride as the basis for compositionally diverse lipids
Nardo, Akers, Cheung et al
RSC Adv (2021) 11 (40), 24752-24761 DOI: 10.1039/d1ra02425f
Nardo, Akers, Cheung et al
RSC Adv (2021) 11 (40), 24752-24761 DOI: 10.1039/d1ra02425f
Cited by 8
AI Summary: OriGene products contributed to this paper by providing the pCMV6-Entry vector (OriGene, Cat# PS100001), which was used for plasmid delivery experiments to assess the gene delivery capabilities of the synthesized lipids.
Showing 1-2 of 2 papers.
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