MTGR1 (CBFA2T2) (NM_001039709) Human Tagged ORF Clone
- TrueORF®
SKU
RG202013
CBFA2T2 (tGFP-tagged) - Human core-binding factor, runt domain, alpha subunit 2; translocated to, 2 (CBFA2T2), transcript variant 4
$489.00
$772.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | MTGR1 |
| Synonyms | EHT; MTGR1; p85; ZMYND3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001039709 |
| ORF Size | 1842 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001039709.1, NP_001034798.1 |
| RefSeq Size | 7449 bp |
| RefSeq ORF | 1728 bp |
| Locus ID | 9139 |
| UniProt ID | O43439 |
| Cytogenetics | 20q11.21-q11.22 |
| Protein Families | Transcription Factors |
| Summary | In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001039709" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202013 | CBFA2T2 (Myc-DDK-tagged)-Human core-binding factor, runt domain, alpha subunit 2, translocated to, 2 (CBFA2T2), transcript variant 4 | 10 ug |
$572.00
|
|
| RC202013L3 | Lenti ORF clone of Human core-binding factor, runt domain, alpha subunit 2; translocated to, 2 (CBFA2T2), transcript variant 4, Myc-DDK-tagged | 10 ug |
$872.00
|
|
| RC202013L4 | Lenti ORF clone of Human core-binding factor, runt domain, alpha subunit 2; translocated to, 2 (CBFA2T2), transcript variant 4, mGFP tagged | 10 ug |
$872.00
|
|
| SC310836 | CBFA2T2 (untagged)-Human core-binding factor, runt domain, alpha subunit 2, translocated to, 2 (CBFA2T2), transcript variant 4 | 10 ug |
$590.00
|
|
Citations
Integrative Genomics of Human Cerebellar Development and Group 3/4 Medulloblastoma
Hendrikse
Thesis (2023)
Hendrikse
Thesis (2023)
Snippet: …HDMB03 was kindly provided by Dr. Till Milde (described in ref. 161) MB3W1 was kindly provided by Dr. Matthias Wölfl (described in ref. 162 ). 3xHA-TurboID-NLS_pCDNA3 was a gift from Dr. Alice Ting (Addgene plasmid # 107171). pCMV6-AC-CBFA2T2-GFP was purchased from Origene (RG202013)
Failure of human rhombic lip differentiation underlies medulloblastoma formation
Hendrikse, Haldipur, Saulnier et al
Nature (2022) 609 (7929), 1021-1028 DOI: 10.1038/s41586-022-05215-w
Hendrikse, Haldipur, Saulnier et al
Nature (2022) 609 (7929), 1021-1028 DOI: 10.1038/s41586-022-05215-w
Cited by 169
AI Summary: OriGene’s pCMV6-AC-CBFA2T2-GFP plasmid (RG202013) was used in this study to express CBFA2T2, enabling functional analyses of this gene’s role in medulloblastoma development.
Snippet: 3×HA-TurboID-NLS_pCDNA3 was a gift from A. Ting (Addgene plasmid 107171). pCMV6-AC-CBFA2T2-GFP was purchased from Origene (RG202013). PCR-amplified TurboID and CBFA2T2 coding sequences were introduced into the KpnI/ClaI-linearized pCAG-H2B-mAG-P2A vector using NEBuilder HiFi assembly (New England Biolabs). Non-vector sequences in resultant plasmids pCAG-H2B-mAG-P2A-3×HA
Showing 1-2 of 2 papers.
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