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DEPP1 (NM_007021) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG202009
C10orf10 (tGFP-tagged) - Human chromosome 10 open reading frame 10 (C10orf10)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $500.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DEPP1
Synonyms C10orf10; DEPP; FIG; Fseg
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG202009 representing NM_007021
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGTCCCGGCTTCTGCTCTCCGTGGCCCATCTGCCCACAATTCGGGAGACCACGGAGGAGATGCTGC
TTGGGGGTCCTGGACAGGAGCCCCCACCCTCTCCTAGCCTGGATGACTACGTGAGGTCTATATCTCGACT
GGCACAGCCCACCTCTGTGCTAGACAAGGCCACGGCCCAGGGCCAACCCAGGCCACCCCACAGGCCAGCC
CAGGCCTGCCGGAAGGGCCGCCCTGCTGTGTCCCTGCGAGACATCACCGCACGTTTCAGTGGCCAGCAGC
CCACACTGCCCATGGCTGATACTGTGGACCCCCTGGACTGGCTTTTTGGGGAGTCCCAGGAAAAGCAGCC
AAGCCAGAGGGACCTGCCAAGGAGGACTGGCCCCTCTGCTGGCCTCTGGGGTCCACATAGACAGATGGAC
AGCAGCAAGCCCATGGGGGCCCCCAGAGGGAGGCTCTGTGAAGCCAGGATGCCTGGGCATTCCCTGGCAA
GACCACCGCAGGATGGGCAGCAGAGCTCTGACCTAAGAAGCTGGACTTTTGGGCAGTCTGCCCAAGCCAT
GGCCTCCCGCCACCGCCCCCGCCCCAGCAGTGTCCTCAGAACACTCTACTCGCACCTCCCGGTGATCCAT
GAACTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG202009 representing NM_007021
Red=Cloning site Green=Tags(s)

MRSRLLLSVAHLPTIRETTEEMLLGGPGQEPPPSPSLDDYVRSISRLAQPTSVLDKATAQGQPRPPHRPA
QACRKGRPAVSLRDITARFSGQQPTLPMADTVDPLDWLFGESQEKQPSQRDLPRRTGPSAGLWGPHRQMD
SSKPMGAPRGRLCEARMPGHSLARPPQDGQQSSDLRSWTFGQSAQAMASRHRPRPSSVLRTLYSHLPVIH
EL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007021
ORF Size 636 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007021.2, NP_008952.1
RefSeq Size 2012 bp
RefSeq ORF 639 bp
Locus ID 11067
UniProt ID Q9NTK1
Cytogenetics 10q11.21
Summary The expression of this gene is induced by fasting as well as by progesterone. The protein encoded by this gene contains a t-synaptosome-associated protein receptor (SNARE) coiled-coil homology domain and a peroxisomal targeting signal. Production of the encoded protein leads to phosphorylation and activation of the transcription factor ELK1. provided by RefSeq, Jul 2008
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"NM_007021" in other vectors (7)
SKU Description Size Price
RC202009 C10orf10 (Myc-DDK-tagged)-Human chromosome 10 open reading frame 10 (C10orf10) 10 ug
$300.00
RC202009L1 Lenti ORF clone of Human chromosome 10 open reading frame 10 (C10orf10), Myc-DDK-tagged 10 ug
$600.00
RC202009L2 Lenti ORF clone of Human chromosome 10 open reading frame 10 (C10orf10), mGFP tagged 10 ug
$600.00
RC202009L3 Lenti ORF clone of Human chromosome 10 open reading frame 10 (C10orf10), Myc-DDK-tagged 10 ug
$600.00
RC202009L4 Lenti ORF clone of Human chromosome 10 open reading frame 10 (C10orf10), mGFP tagged 10 ug
$600.00
SC115744 C10orf10 (untagged)-Human chromosome 10 open reading frame 10 (C10orf10) 10 ug
$300.00
SC322284 C10orf10 (untagged)-Human chromosome 10 open reading frame 10 (C10orf10) 10 ug
$300.00

Plasmid Map for RG202009

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file-download TXT (FASTA format)

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Cloning scheme for RG202009
Circular map for RG202009

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